dnaG:Gene: Difference between revisions

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temperature sensitive
temperature sensitive
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PMID:3536848
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[[cgscmutation:7015|CGSC:7015]]
[[cgscmutation:7015|CGSC:7015]]

Revision as of 16:38, 12 October 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaG

Mnemonic

DNA

Synonyms

ECK3056, b3066, JW3038, dnaP, parB, sdgA[1], sdgA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

69.17 minutes 

MG1655: 3209129..3210874
<gbrowseImage> name=NC_000913:3209129..3210874 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3143924..3145669
<gbrowseImage> name=NC_012967:3143924..3145669 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3096277..3098022
<gbrowseImage> name=NC_012759:3096277..3098022 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3209763..3211508
<gbrowseImage> name=AC_000091:3209763..3211508 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3306874..3308619
<gbrowseImage> name=NC_010473:3306874..3308619 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3209132

Edman degradation

PMID:6750604[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdnaG (Keio:JW3038)

deletion

deletion

PMID:16738554[3]

Shigen

dnaG::Tn5KAN-I-SceI (FB21017)

Insertion at nt 798 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21017

does not contain pKD46

dnaG18

CGSC:5755

dnaG3(ts)

temperature sensitive

PMID:3536848[5]

CGSC:7015

dnaG308(ts)

temperature sensitive

CGSC:9400

dnaG399(ts)

temperature sensitive

CGSC:9406


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Notes

The Keio collection[3] lists a deletion of dnaG. The insertion in this strain is a duplication of the dnaG region.[6]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3038

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGCTGGACGAATCCCACGCGT

Primer 2:CCtTTTTTCGCCAGCTCCTGGTT

19F2

Kohara Phage

Genobase

PMID:3038334[8]

tolC210::Tn10

Linked marker

CAG12184 = CGSC7437[9]

est. P1 cotransduction: 30% [10]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[9]

est. P1 cotransduction: 81% [10]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

pGNG1

Plasmid Clone

PMID:2055480[11]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:EG10239

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG10239

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947570

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Smiley, BL et al. (1982) Sequences of the Escherichia coli dnaG primase gene and regulation of its expression. Proc. Natl. Acad. Sci. U.S.A. 79 4550-4 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Norris, V et al. (1986) DNA replication termination in Escherichia coli parB (a dnaG allele), parA, and gyrB mutants affected in DNA distribution. J. Bacteriol. 168 494-504 PubMed
  6. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  11. ↑ Godson, GN (1991) An over-expression plasmid for Escherichia coli primase. Gene 100 59-64 PubMed

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