dnaG:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DnaG

Synonyms

DNA primase[1], B3066[2][1], SdgA[2][1], ParB[2][1], DnaP[2][1], DnaG[2][1] , dnaP, ECK3056, JW3038, parB, sdgA, b3066

Product description

DNA primase[2][3];

Component of primosome[3]

Primase for DNA replication; primer synthesis for leading- and lagging-strand synthesis; binds Zn(II)[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006154

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002694

F

Seeded from EcoCyc (v14.0)

complete

GO:0003896

DNA primase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002694

F

Seeded from EcoCyc (v14.0)

complete

GO:0003896

DNA primase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006295

F

Seeded from EcoCyc (v14.0)

complete

GO:0003896

DNA primase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013173

F

Seeded from EcoCyc (v14.0)

complete

GO:0005658

alpha DNA polymerase:primase complex

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0639

C

Seeded from EcoCyc (v14.0)

complete

GO:0006259

DNA metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006154

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002694

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0235

P

Seeded from EcoCyc (v14.0)

complete

GO:0006269

DNA replication, synthesis of RNA primer

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006295

P

Seeded from EcoCyc (v14.0)

complete

GO:0006269

DNA replication, synthesis of RNA primer

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013173

P

Seeded from EcoCyc (v14.0)

complete

GO:0006269

DNA replication, synthesis of RNA primer

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0639

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002694

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:1511009[5]

IDA: Inferred from Direct Assay

F

complete

GO:0006269

DNA replication, synthesis of RNA primer

PMID:340457[6]

IDA: Inferred from Direct Assay

P

complete

GO:0005737

cytoplasm

PMID:340457[6]

IDA: Inferred from Direct Assay

C

complete

GO:0003896

DNA primase activity

PMID:340457[6]

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of primosome

could be indirect

Protein

rplA

PMID:15690043[7]

Experiment(s):EBI-885817

Protein

nusG

PMID:15690043[7]

Experiment(s):EBI-890837

Protein

rplL

PMID:15690043[7]

Experiment(s):EBI-890837

Protein

rpsG

PMID:15690043[7]

Experiment(s):EBI-890837

Protein

yfiF

PMID:15690043[7]

Experiment(s):EBI-890837

Protein

ygdH

PMID:15690043[7]

Experiment(s):EBI-890837

Protein

rho

PMID:16606699[8]

Experiment(s):EBI-1144696

Protein

flu

PMID:16606699[8]

Experiment(s):EBI-1144696

Protein

aspS

PMID:16606699[8]

Experiment(s):EBI-1144696

Protein

ybcW

PMID:16606699[8]

Experiment(s):EBI-1144696

Protein

ygdH

PMID:19402753[9]

LCMS(ID Probability):99.0

Protein

DnaB

PMID:8917517[10]

ELISA

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAGRIPRVFI NDLLARTDIV DLIDARVKLK KQGKNFHACC PFHNEKTPSF TVNGEKQFYH
CFGCGAHGNA IDFLMNYDKL EFVETVEELA AMHNLEVPFE AGSGPSQIER HQRQTLYQLM
DGLNTFYQQS LQQPVATSAR QYLEKRGLSH EVIARFAIGF APPGWDNVLK RFGGNPENRQ
SLIDAGMLVT NDQGRSYDRF RERVMFPIRD KRGRVIGFGG RVLGNDTPKY LNSPETDIFH
KGRQLYGLYE AQQDNAEPNR LLVVEGYMDV VALAQYGINY AVASLGTSTT ADHIQLLFRA
TNNVICCYDG DRAGRDAAWR ALETALPYMT DGRQLRFMFL PDGEDPDTLV RKEGKEAFEA
RMEQAMPLSA FLFNSLMPQV DLSTPDGRAR LSTLALPLIS QVPGETLRIY LRQELGNKLG
ILDDSQLERL MPKAAESGVS RPVPQLKRTT MRILIGLLVQ NPELATLVPP LENLDENKLP
GLGLFRELVN TCLSQPGLTT GQLLEHYRGT NNAATLEKLS MWDDIADKNI AEQTFTDSLN
HMFDSLLELR QEELIARERT HGLSNEERLE LWTLNQELAK K
Length

581

Mol. Wt

65.566 kDa

pI

5.9 (calculated)

Extinction coefficient

45,840 - 46,715 (calc based on 16 Y, 4 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

259..341

PF01751 Toprim domain

PMID:19920124[11]

Domain

5..102

PF01807 CHC2 zinc finger

PMID:19920124[11]

Domain

126..252

PF08275 DNA primase catalytic core, N-terminal domain

PMID:19920124[11]

Domain

450..575

PF08278 DNA primase DnaG DnaB-binding

PMID:19920124[11]

Domain

368..426

PF10410 DnaB-helicase binding domain of primase

PMID:19920124[11]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dnaG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

  • Purification of DnaG is described in reference[6]
  • Km data

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130962

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947570

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010065

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ABS5

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10239

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10239

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947570

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000233

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0235

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Stamford, NP et al. (1992) Enriched sources of Escherichia coli replication proteins. The dnaG primase is a zinc metalloprotein. Biochim. Biophys. Acta 1132 17-25 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 Rowen, L & Kornberg, A (1978) Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains. J. Biol. Chem. 253 758-64 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ Lu, YB et al. (1996) Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA. Proc. Natl. Acad. Sci. U.S.A. 93 12902-7 PubMed
  11. ↑ 11.0 11.1 11.2 11.3 11.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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