glcB:Gene: Difference between revisions

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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
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[[MG1655]]
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3119659
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Edman degradation
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PMID:7925370<br />
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Revision as of 19:17, 11 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glcB

Mnemonic

Glycolate

Synonyms

ECK2970, b2976, JW2943, glc[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

67.24 minutes 

MG1655: 3121827..3119656
gbrowse_img.php?coord=3119656..3121827&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3050697..3048526
gbrowse_img.php?coord=3048526..3050697&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 3006804..3008975
gbrowse_img.php?coord=3006804..3008975&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 3122461..3120290
gbrowse_img.php?coord=3120290..3122461&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3219572..3217401
gbrowse_img.php?coord=3217401..3219572&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3119659

Edman degradation

PMID:7925370[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglcB (Keio:JW2943)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10272[4]

ΔglcB::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[5]

glcB11

CGSC:12112

ΔglcB749::kan

PMID:16738554[3]

CGSC:101130



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2943

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGTCAAACCATAACCCAGAG

Primer 2:CCATGACTTTCTTTTTCGCGTAA

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[4]

est. P1 cotransduction: 57% [7]

metC162::Tn10

Linked marker

CAG18475 = CGSC7435[4]

est. P1 cotransduction: 30% [7]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG20080

EchoBASE (EcoliWiki Page)

EchoBASE:EB4141

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2976

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3705

EcoGene (EcoliWiki Page)

EcoGene:EG20080

CGSC (EcoliWiki Page)

CGSC:711

ASAP (EcoliWikiPage)

ASAP:ABE-0009767



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Molina, I et al. (1994) Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme. Eur. J. Biochem. 224 541-8 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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