glcB:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glcB

Mnemonic

Glycolate

Synonyms

ECK2970, b2976, JW2943, glc[1], glc

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

67.24 minutes 

MG1655: 3121827..3119656
<gbrowseImage> name=NC_000913:3119656..3121827 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3050697..3048526
<gbrowseImage> name=NC_012967:3048526..3050697 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3006804..3008975
<gbrowseImage> name=NC_012759:3006804..3008975 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3122461..3120290
<gbrowseImage> name=NC_007779:3120290..3122461 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3219572..3217401
<gbrowseImage> name=NC_010473:3217401..3219572 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3119659

Edman degradation

PMID:7925370[2]


<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglcB (Keio:JW2943)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10272[4]

ΔglcB::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[5]

glcB11

CGSC:12112

ΔglcB749::kan

PMID:16738554[3]

CGSC:101130


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2943

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGTCAAACCATAACCCAGAG

Primer 2:CCATGACTTTCTTTTTCGCGTAA

1G7

Kohara Phage

Genobase

PMID:3038334[7]

12F3

Kohara Phage

Genobase

PMID:3038334[7]

2F9

Kohara Phage

Genobase

PMID:3038334[7]

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[4]

est. P1 cotransduction: 57% [8]

metC162::Tn10

Linked marker

CAG18475 = CGSC7435[4]

est. P1 cotransduction: 30% [8]

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG20080

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20080

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002441

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948857

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4141

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009767

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Molina, I et al. (1994) Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme. Eur. J. Biochem. 224 541-8 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories