proA:Gene: Difference between revisions

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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
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[[MG1655]]
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260727
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Edman degradation
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PMID:6089111<br />
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Revision as of 19:04, 11 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

proA

Mnemonic

Proline

Synonyms

ECK0244, b0243, JW0233, pro1, pro(1)[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.62 minutes 

MG1655: 260727..261980
gbrowse_img.php?coord=260727..261980&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 265995..267248
gbrowse_img.php?coord=265995..267248&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 260726..261979
gbrowse_img.php?coord=260726..261979&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 260727..261980
gbrowse_img.php?coord=260727..261980&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 234831..236084
gbrowse_img.php?coord=234831..236084&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

260727

Edman degradation

PMID:6089111[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔproA (Keio:JW0233)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8468[4]

proA

PMID:12519969[5]

ASAP

At position 192 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20067

proA81::Tn10

Insertion at 261,286 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG18447 = CGSC7330[4]

Synonyms:

proA761(del)::kan

insertion

insertion

proA23

CGSC:5573

proA44

CGSC:6453

proA81::Tn10

PMID:9829956[7]

CGSC:7088

proA35

CGSC:8556

proA30

CGSC:11535

proA181

CGSC:12290

proA180

CGSC:12291

proA

CGSC:14522

proA3096::Tn10kan

PMID:2540407[8] PMID:9829956[7]

CGSC:38994

ΔproA761::kan

PMID:16738554[3]

CGSC:102466


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0233

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCCTGGAACAAATGGGCATTGC

Primer 2:CCCGCACGAATGGTGTAATCACC

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[4]

est. P1 cotransduction: 33% [6]
Synonyms:zae-502::Tn10

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[4]

est. P1 cotransduction: % [6]
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10767

EchoBASE (EcoliWiki Page)

EchoBASE:EB0760

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0243

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0390

EcoGene (EcoliWiki Page)

EcoGene:EG10767

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PROA

CGSC (EcoliWiki Page)

CGSC:364

ASAP (EcoliWikiPage)

ASAP:ABE-0000832


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Deutch, AH et al. (1984) Analysis of the Escherichia coli proBA locus by DNA and protein sequencing. Nucleic Acids Res. 12 6337-55 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
  5. ↑ Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  6. ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
  8. ↑ Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed

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