mutH:Gene: Difference between revisions

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Revision as of 21:19, 26 July 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mutH

Mnemonic

Mutator

Synonyms

ECK2827, b2831, JW2799, mutR, prv[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.96 minutes 

MG1655: 2967684..2968373
<gbrowseImage> name=NC_000913:2967684..2968373 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2864282..2864971
<gbrowseImage> name=NC_012967:2864282..2864971 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2854832..2855521
<gbrowseImage> name=NC_012759:2854832..2855521 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2968318..2969007
<gbrowseImage> name=AC_000091:2968318..2969007 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3061554..3062243
<gbrowseImage> name=NC_010473:3061554..3062243 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2967687

Edman degradation

PMID:3031619[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmutH (Keio:JW2799)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10186[4]

mutH::Tn5KAN

PMID:12519969[5]

ASAP

At position 200 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20953

mutH::Tn5KAN

PMID:12519969[5]

ASAP

At position 200 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20954

mutHQ135K

Q135K

(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64)

Strain variation; seeded from UniProt:P06722

mutHG64R

G64R

(in strain: ECOR 50)

Strain variation; seeded from UniProt:P06722

mutHD157N

D157N

In TS28; temperature-sensitive

seeded from UniProt:P06722

mutHQ153R

Q153R

(in strain: ECOR 67)

Strain variation; seeded from UniProt:P06722

mutHR155C

R155C

(in strain: ECOR 35)

Strain variation; seeded from UniProt:P06722

mutHT28L

T28L

In TS7; temperature-sensitive

seeded from UniProt:P06722

mutHW58S

W58S

(in strain: ECOR 67)

Strain variation; seeded from UniProt:P06722

mutHG60C

G60C

(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70)

Strain variation; seeded from UniProt:P06722

mutH34

PMID:1092652[6]

CGSC:12133

mutH471::kan

CGSC:30821

mutH472::mini-Tn10

PMID:1649454[7]

CGSC:38814

ΔmutH756::kan

PMID:16738554[3]

CGSC:101972



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2799

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTCCCAACCTCGCCCACTGCT

Primer 2:CCtTGGATCAGAAAATGACGGGC

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[4]

est. P1 cotransduction: 55% [9]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[4]

est. P1 cotransduction: % [9]
Synonyms:zgd-210::Tn10, zgf-210::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10624

EchoBASE (EcoliWiki Page)

EchoBASE:EB0619

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2831

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3426

EcoGene (EcoliWiki Page)

EcoGene:EG10624

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MUTH

CGSC (EcoliWiki Page)

CGSC:471

ASAP (EcoliWikiPage)

ASAP:ABE-0009287



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Grafstrom, RH & Hoess, RH (1987) Nucleotide sequence of the Escherichia coli mutH gene. Nucleic Acids Res. 15 3073-84 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  6. ↑ Hoess, RH & Herman, RK (1975) Isolation and characterization of mutator strains of Escherichia coli K-12. J. Bacteriol. 122 474-84 PubMed
  7. ↑ Michaels, ML et al. (1991) MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19 3629-32 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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