mutH:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
mutH |
|---|---|
| Mnemonic |
Mutator |
| Synonyms |
ECK2827, b2831, JW2799, mutR, prv[1], prv |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
63.96 minutes |
MG1655: 2967684..2968373 |
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NC_012967: 2864282..2864971 |
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NC_012759: 2854832..2855521 |
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W3110 |
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W3110: 2968318..2969007 |
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DH10B: 3061554..3062243 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2967687 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔmutH (Keio:JW2799) |
deletion |
deletion |
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mutH::Tn5KAN-I-SceI (FB20953) |
Insertion at nt 200 in Minus orientation |
contains pKD46 | |||||
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mutH::Tn5KAN-I-SceI (FB20954) |
Insertion at nt 200 in Minus orientation |
does not contain pKD46 | |||||
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mutHQ135K |
Q135K |
(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64) |
Strain variation; seeded from UniProt:P06722 | ||||
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mutHG64R |
G64R |
(in strain: ECOR 50) |
Strain variation; seeded from UniProt:P06722 | ||||
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mutHD157N |
D157N |
In TS28; temperature-sensitive |
seeded from UniProt:P06722 | ||||
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mutHQ153R |
Q153R |
(in strain: ECOR 67) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHR155C |
R155C |
(in strain: ECOR 35) |
Strain variation; seeded from UniProt:P06722 | ||||
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mutHT28L |
T28L |
In TS7; temperature-sensitive |
seeded from UniProt:P06722 | ||||
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mutHW58S |
W58S |
(in strain: ECOR 67) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHG60C |
G60C |
(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70) |
Strain variation; seeded from UniProt:P06722 | ||||
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mutH34 |
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mutH471::kan |
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mutH472::mini-Tn10 |
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ΔmutH756::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2799 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCCCAACCTCGCCCACTGCT Primer 2:CCtTGGATCAGAAAATGACGGGC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 55% [10] | ||
|
Linked marker |
est. P1 cotransduction: % [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Grafstrom, RH & Hoess, RH (1987) Nucleotide sequence of the Escherichia coli mutH gene. Nucleic Acids Res. 15 3073-84 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Hoess, RH & Herman, RK (1975) Isolation and characterization of mutator strains of Escherichia coli K-12. J. Bacteriol. 122 474-84 PubMed
- ↑ Michaels, ML et al. (1991) MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19 3629-32 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
