PMID:1649454
| Citation |
Michaels, ML, Pham, L, Cruz, C and Miller, JH (1991) MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19:3629-32 |
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| Abstract |
We have cloned chromosomal DNA bordering an insert that inactivates mutM. Sequencing of this clone has revealed that the insertion element is located between the promoter and structural gene for formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase). An overproducing clone of Fapy-DNA glycosylase complements the original mutM strain that had been isolated after EMS mutagenesis. Thus, we conclude that MutM is actually Fapy-DNA glycosylase. mutM has previously been characterized as a mutator strain that leads specifically to G.C----T.A transversions. This in vivo characterization correlates well with the mutagenic potential of one of the lesions Fapy-DNA glycosylase removes, 8-oxo-7,8-dihydro-2'-deoxyguanine (8-OxodG). |
| Links | |
| Keywords |
Bacteriophages/genetics; Base Sequence; Cloning, Molecular; DNA Repair/genetics; DNA Repair/physiology; DNA Transposable Elements/genetics; DNA-Formamidopyrimidine Glycosylase; Deoxyguanosine/analogs & derivatives; Deoxyguanosine/toxicity; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Genetic Complementation Test; Molecular Sequence Data; Mutagenesis, Insertional/genetics; N-Glycosyl Hydrolases/genetics; Plasmids/genetics; Promoter Regions, Genetic/genetics; Recombinant Proteins/biosynthesis |
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