mutH:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MutH

Synonyms

methyl-directed mismatch repair protein[1], B2831[2][1], Prv[2][1], MutR[2][1], MutH[2][1] , ECK2827, JW2799, mutR, prv, b2831

Product description

MutH[2][3];

Component of MutHLS complex, methyl-directed mismatch repair[2][3]

Methyl-directed mismatch repair[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006298

mismatch repair

PMID:6987663[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0004519

endonuclease activity

PMID:2824465[6] PMID:1601880[7]

IDA: Inferred from Direct Assay

F

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004230

F

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

PMID:2665076[8]

IDA: Inferred from Direct Assay

P

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011335

F

Seeded from EcoCyc (v14.0)

complete

GO:0032300

mismatch repair complex

PMID:6987663[5]

IGI: Inferred from Genetic Interaction

UniProtKB:P23367|UniProtKB:P23909

C

With MutL (P23367) and MutS (P23909).

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018140

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00759

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004230

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018140

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00759

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00759

P

Seeded from EcoCyc (v14.0)

complete

GO:0006304

DNA modification

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004230

P

Seeded from EcoCyc (v14.0)

complete

GO:0006304

DNA modification

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018140

P

Seeded from EcoCyc (v14.0)

complete

GO:0043765

T/G mismatch-specific endonuclease activity

PMID:12606120[9]

IDA: Inferred from Direct Assay

F

activated by MutS and MutL

complete

GO:0003677

DNA binding

PMID:12606120[9]

IDA: Inferred from Direct Assay

F

complete

GO:0006298

mismatch repair

PMID:12606120[9]

IDA: Inferred from Direct Assay

P

complete

GO:0000018

PMID:2555716[10]

IMP: Inferred from Mutant Phenotype

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of MutHLS complex, methyl-directed mismatch repair

could be indirect

Protein

rplF

PMID:16606699[11]

Experiment(s):EBI-1144107

Protein

rplB

PMID:16606699[11]

Experiment(s):EBI-1144107

Protein

uvrY

PMID:16606699[11]

Experiment(s):EBI-1144107

Protein

napC

PMID:16606699[11]

Experiment(s):EBI-1144107

Protein

rplQ

PMID:16606699[11]

Experiment(s):EBI-1144107

Protein

slyD

PMID:16606699[11]

Experiment(s):EBI-1144107

Protein

rpsJ

PMID:19402753[12]

LCMS(ID Probability):99.6

Protein

dinJ

PMID:19402753[12]

LCMS(ID Probability):99.6 MALDI(Z-score):26.209733

Protein

MutL

The C-terminal 218 amino acids were sufficient for the two-hybrid interaction with MutH

PMID:9880500[13]

2-Hybrid Screening

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSQPRPLLSP PETEEQLLAQ AQQLSGYTLG ELAALVGLVT PENLKRDKGW IGVLLEIWLG
ASAGSKPEQD FAALGVELKT IPVDSLGRPL ETTFVCVAPL TGNSGVTWET SHVRHKLKRV
LWIPVEGERS IPLAQRRVGS PLLWSPNEEE DRQLREDWEE LMDMIVLGQV ERITARHGEY
LQIRPKAANA KALTEAIGAR GERILTLPRG FYLKKNFTSA LLARHFLIQ
Length

229

Mol. Wt

25.527 kDa

pI

7.0 (calculated)

Extinction coefficient

37,470 - 37,595 (calc based on 3 Y, 6 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P06722

Domain

58..157

PF02976 DNA mismatch repair enzyme MutH

PMID:19920124[14]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mutH taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

MutH structure[15]









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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

Purified protein

MutH E56A[9]

  • No detectable endonuclease activity

Purified protein

MutH D70A[9]

  • No detectable endonuclease activity
  • Slightly reduced DNA binding

Purified protein

MutH E77A[9]

  • No detectable endonuclease activity
  • Slightly reduced DNA binding

Purified protein

MutH K79E[9]

  • No detectable endonuclease activity
  • Slightly reduced DNA binding

Purification protocol

Single-step purification of His6-MutH

PMID:8747662[16]

Purified protein

MutH K116E[9]

  • No detectable endonuclease activity
  • Slightly reduced DNA binding
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130735

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947299

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009287

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P06722

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10624

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10624

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947299

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000617

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0619

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Glickman, BW & Radman, M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc. Natl. Acad. Sci. U.S.A. 77 1063-7 PubMed
  6. ↑ Welsh, KM et al. (1987) Isolation and characterization of the Escherichia coli mutH gene product. J. Biol. Chem. 262 15624-9 PubMed
  7. ↑ Au, KG et al. (1992) Initiation of methyl-directed mismatch repair. J. Biol. Chem. 267 12142-8 PubMed
  8. ↑ Lahue, RS et al. (1989) DNA mismatch correction in a defined system. Science 245 160-4 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 9.6 9.7 Junop, MS et al. (2003) In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination. DNA Repair (Amst.) 2 387-405 PubMed
  10. ↑ Rayssiguier, C et al. (1989) The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature 342 396-401 PubMed
  11. ↑ 11.0 11.1 11.2 11.3 11.4 11.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  12. ↑ 12.0 12.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  13. ↑ Hall, MC & Matson, SW (1999) The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity. J. Biol. Chem. 274 1306-12 PubMed
  14. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
  15. ↑ Ban, C & Yang, W (1998) Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases. EMBO J. 17 1526-34 PubMed
  16. ↑ Feng, G & Winkler, ME (1995) Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12. BioTechniques 19 956-65 PubMed

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