proA:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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****************************************************************************************** -->{{Gene_nomenclature_help}} | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| | {| id="C46a9aba28ba50" class=" tableEdit Gene_nomenclature_table" | ||
|- | |- | ||
!align=left {{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
|| | || | ||
''proA'' | ''proA'' | ||
|- | |- | ||
!align=left {{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
|| | || | ||
Proline | |||
|- | |- | ||
!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK0244, b0243, JW0233, pro1, pro(1)''<ref name='LIB:Riley_2006'/> | ''ECK0244, b0243, JW0233, pro1, pro(1)''<ref name='LIB:Riley_2006'/>, pro'' | ||
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****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a9aba3431ee" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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5.62 minutes | 5.62 minutes | ||
| | | | ||
MG1655: 260727..261980<br /> | MG1655: 260727..261980<br /><gbrowseImage> | ||
name=NC_000913:260727..261980 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10767 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10767 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|259727|262980|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|260727|261980|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10767 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10767 EcoCyc Sequence Dump] | ||
|- | |||
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[[REL606]] | |||
| | |||
| | |||
NC_012967: 265995..267248<br /><gbrowseImage> | |||
name=NC_012967:265995..267248 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|REL606|NC_012967|265995|267248|GBrowse (REL606)}} | |||
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{{GBrowseFastaLink|REL606|NC_012967|265995|267248|FASTA Dump}} | |||
|- | |||
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[[BW2952]] | |||
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NC_012759: 260726..261979<br /><gbrowseImage> | |||
name=NC_012759:260726..261979 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|260726|261979|GBrowse (BW2952)}} | |||
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{{GBrowseFastaLink|BW2952|NC_012759|260726|261979|FASTA Dump}} | |||
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W3110: 260727..261980<br />{{ | W3110: 260727..261980<br /><gbrowseImage> | ||
name=NC_007779:260727..261980 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|W3110|NC_007779|259727|262980|GBrowse(W3110)}} | |||
| | |||
{{GBrowseFastaLink|W3110|NC_007779|260727|261980|FASTA Dump}} | |||
|- | |||
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[[DH10B]] | |||
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| | |||
DH10B: 234831..236084<br /><gbrowseImage> | |||
name=NC_010473:234831..236084 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|232325|238590|GBrowse(DH10B)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|232325|238590|FASTA Dump}} | ||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="D46a9aba4440d7" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|- | |||
| | |||
Coding Start (SO:0000323) | |||
| | |||
[[MG1655]] | |||
| | |||
260727 | |||
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Edman degradation | |||
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PMID:6089111<br /> | |||
| | |||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a9aba4b525d" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|- | |- | ||
| | | | ||
ΔproA (Keio:JW0233) | |||
| | | | ||
deletion | deletion | ||
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deletion | deletion | ||
| | | | ||
Auxotrophies | |||
| | |||
Requires proline for growth | |||
| | |||
PMID:16738554 | |||
| | |||
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | |||
[http://cgsc2.biology.yale.edu/Strain.php?ID=106749 CGSC8468]<ref name='LIB:cgsc'/> | |||
| | |||
|- | |||
| | |||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0000832 proA::Tn5KAN-2] (FB20067) | |||
| | |||
Insertion at nt 192 in Plus orientation | |||
| | | | ||
| | | | ||
Auxotrophies | |||
| | |||
Requires proline for growth | |||
| | |||
PMID:15262929 | |||
| | | | ||
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20067] | |||
| | |||
does not contain [[pKD46]] | |||
|- | |- | ||
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Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
adapted from Nichols ''et al.''<ref name ='LIB:Nichols_linked_Tn'/> | adapted from Nichols ''et al.''<ref name ='LIB:Nichols_linked_Tn'/> | ||
| Line 178: | Line 271: | ||
insertion | insertion | ||
| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
| Line 195: | Line 288: | ||
| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | |||
Requires proline for growth | |||
| | | | ||
PMID:9829956 | |||
| | | | ||
[[cgscmutation:7088|CGSC:7088]] | [[cgscmutation:7088|CGSC:7088]] | ||
| Line 245: | Line 339: | ||
| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | | | ||
Requires proline for growth | |||
| | | | ||
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| | | | ||
Auxotrophies | |||
| | |||
Requires proline for growth | |||
| | | | ||
PMID:2540407 PMID:9829956 | |||
| | | | ||
[[cgscmutation:38994|CGSC:38994]] | [[cgscmutation:38994|CGSC:38994]] | ||
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|- | |- | ||
| | | | ||
'' | ''ΔproA761::kan'' | ||
| | |||
deletion | |||
| | |||
deletion | |||
| | |||
Auxotrophies | |||
| | |||
Requires proline for growth | |||
| | |||
PMID:16738554 | |||
| | |||
[[cgscmutation:102466|CGSC:102466]] | |||
| | |||
|- | |||
| | |||
''proA'' | |||
| | |||
| | | | ||
| | |||
Resistant to | |||
| | |||
Resistant to Nitro(4)pyridine N-oxide | |||
| | |||
PMID:4598010 | |||
| | | | ||
| | |||
|- | |||
| | |||
''proA'' | |||
| | | | ||
| | | | ||
| | | | ||
Resistant to | |||
| | |||
Nitro-4-pyridine N-oxide Resistance | |||
| | |||
PMID:385587 | |||
| | |||
Strain: W3630 | |||
| | | | ||
table 1 | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.11065.Y4e04c17f26673--> | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y4e04c17f26673" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.11065.Y4e04c17f26673&page=11065&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="R46a9aba5a39bf" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | ||
| | | | ||
PMID:16769691 | |||
Status:Clone OK | Status:Clone OK | ||
| Line 390: | Line 543: | ||
Primer 2:CCCGCACGAATGGTGTAATCACC | Primer 2:CCCGCACGAATGGTGTAATCACC | ||
|- | |||
| | |||
[[:Category:Kohara_Phage:8F9|8F9]] | |||
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Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=127 Genobase] | |||
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PMID:3038334 | |||
|- | |||
| | |||
[[:Category:Kohara_Phage:8G4|8G4]] | |||
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Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=128 Genobase] | |||
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PMID:3038334 | |||
|- | |- | ||
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| Line 398: | Line 569: | ||
CAG18436 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38989 CGSC7328]<ref name='LIB:cgsc'/> | CAG18436 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38989 CGSC7328]<ref name='LIB:cgsc'/> | ||
| | | | ||
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:zae-502::Tn10 | |||
|- | |- | ||
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| Line 407: | Line 579: | ||
CAG18439 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39002 CGSC7334]<ref name='LIB:cgsc'/> | CAG18439 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39002 CGSC7334]<ref name='LIB:cgsc'/> | ||
| | | | ||
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC). | |||
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****************************************************************************************** -->{{Gene_accessions_help}} | ****************************************************************************************** -->{{Gene_accessions_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9aba691c43" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[[EcoCyc]] | |||
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EcoCyc:EG10767 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[EcoGene]] | |||
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EcoGene:EG10767 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000758 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=946680 GeneID:946680] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0760 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0000832 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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==References== | ==References== | ||
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__NOTOC__ | __NOTOC__ | ||
===Categories=== | ===Categories=== | ||
[[Category:Gene List:MG1655]] | [[Category:Gene List:MG1655]] | ||
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[[Category:Genes in the Keio knockout collection]] | [[Category:Genes in the Keio knockout collection]] | ||
[[Category:Genes in the ASKA clone collection]] | [[Category:Genes in the ASKA clone collection]] | ||
[[Category:pro genes]] | [[Category:pro genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | ||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:28, 19 September 2013
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
proA |
|---|---|
| Mnemonic |
Proline |
| Synonyms |
ECK0244, b0243, JW0233, pro1, pro(1)[1], pro |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
5.62 minutes |
MG1655: 260727..261980 |
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|
NC_012967: 265995..267248 |
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|
NC_012759: 260726..261979 |
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|
W3110 |
|
W3110: 260727..261980 |
||
|
DH10B: 234831..236084 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
260727 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔproA (Keio:JW0233) |
deletion |
deletion |
Auxotrophies |
Requires proline for growth |
|||
|
proA::Tn5KAN-2 (FB20067) |
Insertion at nt 192 in Plus orientation |
Auxotrophies |
Requires proline for growth |
does not contain pKD46 | |||
|
proA81::Tn10 |
Insertion at 261,286 bp in MG1655 (NC_000913) |
Auxotrophies |
Requires proline for growth |
adapted from Nichols et al.[6] |
Synonyms: | ||
|
proA761(del)::kan |
insertion |
insertion |
Auxotrophies |
Requires proline for growth |
|||
|
proA23 |
Auxotrophies |
Requires proline for growth |
|||||
|
proA44 |
Auxotrophies |
Requires proline for growth |
|||||
|
proA81::Tn10 |
Auxotrophies |
Requires proline for growth |
|||||
|
proA35 |
Auxotrophies |
Requires proline for growth |
|||||
|
proA30 |
Auxotrophies |
Requires proline for growth |
|||||
|
proA181 |
Auxotrophies |
Requires proline for growth |
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|
proA180 |
Auxotrophies |
Requires proline for growth |
|||||
|
proA |
Auxotrophies |
Requires proline for growth |
|||||
|
proA3096::Tn10kan |
Auxotrophies |
Requires proline for growth |
|||||
|
ΔproA761::kan |
deletion |
deletion |
Auxotrophies |
Requires proline for growth |
|||
|
proA |
Resistant to |
Resistant to Nitro(4)pyridine N-oxide |
|||||
|
proA |
Resistant to |
Nitro-4-pyridine N-oxide Resistance |
Strain: W3630 |
table 1 | |||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0233 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCTGGAACAAATGGGCATTGC Primer 2:CCCGCACGAATGGTGTAATCACC | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 33% [6] | ||
|
Linked marker |
est. P1 cotransduction: % [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Deutch, AH et al. (1984) Analysis of the Escherichia coli proBA locus by DNA and protein sequencing. Nucleic Acids Res. 12 6337-55 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 7.0 7.1 Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
- ↑ Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
- ↑ Berg, CM & Rossi, JJ (1974) Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 118 928-34 PubMed
- ↑ Fayerman, JT et al. (1979) ilvU, a locus in Escherichia coli affecting the derepression of isoleucyl-tRNA synthetase and the RPC-5 chromatographic profiles of tRNAIle and tRNAVal. J. Biol. Chem. 254 9429-40 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 12.0 12.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed