ompA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|   id="B46a9bc7cf1ca3"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''ompA''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''ompA''
Outer membrane protein
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK0948, b0957, JW0940, con, tolG, tut''<ref name='LIB:Riley_2006'/>, tut''


|-
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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.12473.B46a9bc7cf1ca3&page=12473&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK0948, b0957, JW0940, con, tolG, tut''<ref name='LIB:Riley_2006'/>
|-
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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='130619'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a9bc7d7658c"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
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MG1655
MG1655
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21.95 minutes&nbsp;
21.95 minutes&nbsp;
|
|
MG1655: 1019276..1018236<br />{{GBrowseFigureByCoord|1017236|1020276|ecoli|MG1655}}
MG1655: 1019276..1018236<br /><gbrowseImage>
name=NC_000913:1018236..1019276
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10669 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10669 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|1017236|1020276|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|1017236|1020276|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:1018236..1019276&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|1018236|1019276|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10669 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10669 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 1037144..1036104<br /><gbrowseImage>
name=NC_012967:1036104..1037144
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|1036104|1037144|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|1036104|1037144|FASTA Dump}}
|-
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[[BW2952]]
|
 
|
NC_012759: 921204..922244<br /><gbrowseImage>
name=NC_012759:921204..922244
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|921204|922244|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|921204|922244|FASTA Dump}}
|-  
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W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 1020475..1019435<br />{{GBrowseFigureByCoord|1018435|1021475|ecoli_W3110|W3110}}  
W3110: 1020475..1019435<br /><gbrowseImage>
name=NC_007779:1019435..1020475
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|1018435|1021475|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|1019435|1020475|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
DH10B: 1073204..1072164<br /><gbrowseImage>
name=NC_010473:1072164..1073204
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:1018435..1021475 GBrowse(W3110)]
{{GBrowseLink|DH10B|NC_010473|1070084|1075284|GBrowse(DH10B)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:1019435..1020475&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|DH10B|NC_010473|1070084|1075284|FASTA Dump}}
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-class='sortbottom'
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Coding Start (SO:0000323)
|
[[MG1655]]
|
1018239
|
Edman degradation
|
PMID:340230<br />PMID:7001461<br />PMID:8740179<br />PMID:9298646<br />PMID:9629924<br />
|
 
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====Notes====
====Notes====
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46a9bc7e6156d"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
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&Delta;ompA (Keio:JW0940)
''ompA(del)'' (Keio:JW0940)
|
|
deletion
deletion
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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
 
[http://cgsc2.biology.yale.edu/Strain.php?ID=107223 CGSC8942]<ref name='LIB:cgsc'/>
|
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003240 ompA::Tn5KAN-2] (FB20072)
|
Insertion at nt 518 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20072]
|
does not contain [[pKD46]]
|-
|
''ompA''::kan
|
deletion
|
 
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|Acetate]]
|
PMID:16095938
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107223 CGSC8942]<ref name='LIB:cgsc'/>
 
|
|
   
   
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|-  
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''&#916;ompA772::kan''
''ompA772(del)::kan''
|
|


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|
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[[cgscmutation:102174|CGSC:102174]]
[[cgscmutation:102174|CGSC:102174]]
|
|
   
   
|-
|
''P678-54ompA2101''
|
|
|
Sensitivity to
|
increased sensitivity to acid, table 3.
|
PMID:1314797
|
Experimental strain: RJR200
|
|-
|
''ompA'' in strain P460
|
|
|
Resistant to
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Resistant to bacteriocin Colicin K
|
PMID:1991710
|
Strain: P460
|
table 3
|-
|
''ompA'' in strain P1636
|
|
|
Resistant to
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Resistant to bacteriocin Colicin L
|
PMID:1991710
|
Strain: P460
|
table 3
|-
|
''ompA'' in strain P460
|
|
|
Resistant to
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Resistant to bacteriophage K3
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PMID:1991710
PMID:6819426
|
Strain: p460
|
Figure 6
|-
|
''ompA1''
|
|
|
Resistant to
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Resistant to novobicin
|
PMID:6819426
|
Strains: ompA1, ompA2 and ompA3
|
table 6
|-
|
''ompA1''
|
|
|
Resistant to
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Resistant to chelator, specifically EDTA
|
PMID:6819426
|
Strains: ompA1, ompA2 and ompA3
|
table 6
|-
|
''ompA'' in strain CC205
|
|
|
Resistant to
|
Resistant to Phage TUII<nowiki>*</nowiki>
|
PMID:6819426
|
Strain: CC205
|
Table 4
|-
|
''ompA'' in strain CC205
|
|
|
Resistant to
|
Resistant to phage K3hl
|
PMID:6819426
|
strain CC205
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Table 4
|-
|
''ompA'' in strain CC205
|
|
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Resistant to
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Resistant to pphage K3
|
PMID:6819426
|
Strain: cc205
|
table 4
|-
|
''ompA'' in strain P460
|
|
|
Resistant to
|
Resistance to Bacteriocin JF246
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PMID:783128
|
Strain P460
|
Table 3
|-
|
''con''-''tsx'' in  P1731
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|
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Resistant to
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Resistance to Bacteriophage T6
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PMID:786287
|
|
Double Mutation to the ''con'' & ''tsx'' genes cause resistance to the phage T6, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. 
|-
|
''con''-''tsx'' in  P1731
|
|
|
Resistant to
|
Resistance to Colicin K
|
PMID:786287
|


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Double Mutation to the ''con'' & ''tsx'' genes cause resistance to the Colicin K, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. 
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.12473.I46a9bc7e6156d&page=12473&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || ||
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{{Gene_alleles_help}}
 
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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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JW0940
JW0940
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 238: Line 579:


Primer 2:CCAGCCTGCGGCTGAGTTACAA  
Primer 2:CCAGCCTGCGGCTGAGTTACAA  
|-
|-
|
[[:Category:Kohara_Phage:E2E5|E2E5]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=222 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:9B10|9B10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=223 Genobase]
|
PMID:3038334
|-  
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[[ycbS:Gene|zcb-3059::Tn10]]
[[ycbS:Gene|zcb-3059::Tn10]]
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|
|
est. P1 cotransduction: 57% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 57% <ref name='LIB:Nichols_linked_Tn'/>  
|-
|-  
|
|
[[yccE:Gene|zcc-282::Tn10]]
[[yccE:Gene|zcc-282::Tn10]]
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|
|
est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/>  
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===Notes===
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z46a9bc7f95ebb"  class=" tableEdit Gene_accessions_table"   
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10669 EcoCyc:EG10669]
EcoCyc:EG10669
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0663 EchoBASE:EB0663]
EcoGene:EG10669
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b0957 EcoliGenExpDB:b0957]
RegulonDB:ECK120000662
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C1093 ECOL199310CYC:C1093]
[http://www.ncbi.nlm.nih.gov/gene?term=945571 GeneID:945571]
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Escherichia coli str. K-12 substr. MG1655
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EchoBASE]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10669 EcoGene:EG10669]
EchoBASE:EB0663
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[ASAP]]
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[http://BioCyc.org/ECOO157/new-image?object=OMPA ECOO157CYC:OMPA]
ASAP:ABE-0003240
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/437 CGSC:437]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003240&formSubmitReturn=1 ASAP:ABE-0003240]
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===Categories===
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[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:omp genes]]
[[Category:omp genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:32, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ompA

Mnemonic

Outer membrane protein

Synonyms

ECK0948, b0957, JW0940, con, tolG, tut[1], tut

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

21.95 minutes 

MG1655: 1019276..1018236
<gbrowseImage> name=NC_000913:1018236..1019276 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1037144..1036104
<gbrowseImage> name=NC_012967:1036104..1037144 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 921204..922244
<gbrowseImage> name=NC_012759:921204..922244 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1020475..1019435
<gbrowseImage> name=NC_007779:1019435..1020475 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1073204..1072164
<gbrowseImage> name=NC_010473:1072164..1073204 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1018239

Edman degradation

PMID:340230[2]
PMID:7001461[3]
PMID:8740179[4]
PMID:9298646[5]
PMID:9629924[6]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ompA(del) (Keio:JW0940)

deletion

deletion

PMID:16738554[7]

Shigen

CGSC8942[8]

ompA::Tn5KAN-2 (FB20072)

Insertion at nt 518 in Minus orientation

PMID:15262929[9]

E. coli Genome Project:FB20072

does not contain pKD46

ompA::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[10]

ompA252

CGSC:9906

ompA256

CGSC:9910

ompA772(del)::kan

PMID:16738554[7]

CGSC:102174

P678-54ompA2101

Sensitivity to

increased sensitivity to acid, table 3.

PMID:1314797[11]

Experimental strain: RJR200

ompA in strain P460

Resistant to

Resistant to bacteriocin Colicin K

PMID:1991710[12]

Strain: P460

table 3

ompA in strain P1636

Resistant to

Resistant to bacteriocin Colicin L

PMID:1991710[12]

Strain: P460

table 3

ompA in strain P460

Resistant to

Resistant to bacteriophage K3

PMID:1991710[12]

PMID:6819426[13]

Strain: p460

Figure 6

ompA1

Resistant to

Resistant to novobicin

PMID:6819426[13]

Strains: ompA1, ompA2 and ompA3

table 6

ompA1

Resistant to

Resistant to chelator, specifically EDTA

PMID:6819426[13]

Strains: ompA1, ompA2 and ompA3

table 6

ompA in strain CC205

Resistant to

Resistant to Phage TUII*

PMID:6819426[13]

Strain: CC205

Table 4

ompA in strain CC205

Resistant to

Resistant to phage K3hl

PMID:6819426[13]

strain CC205

Table 4

ompA in strain CC205

Resistant to

Resistant to pphage K3

PMID:6819426[13]

Strain: cc205

table 4

ompA in strain P460

Resistant to

Resistance to Bacteriocin JF246

PMID:783128[14]

Strain P460

Table 3

con-tsx in P1731

Resistant to

Resistance to Bacteriophage T6

PMID:786287[15]

Double Mutation to the con & tsx genes cause resistance to the phage T6, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell.

con-tsx in P1731

Resistant to

Resistance to Colicin K

PMID:786287[15]

Double Mutation to the con & tsx genes cause resistance to the Colicin K, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0940

Plasmid clone

Shigen

PMID:16769691[16]

Status:Clone OK

Primer 1:GCCAAAAAGACAGCTATCGCGAT

Primer 2:CCAGCCTGCGGCTGAGTTACAA

E2E5

Kohara Phage

Genobase

PMID:3038334[17]

9B10

Kohara Phage

Genobase

PMID:3038334[17]

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[8]

est. P1 cotransduction: 57% [18]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[8]

est. P1 cotransduction: 14% [18]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10669

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10669

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000662

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945571

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0663

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003240

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Schweizer, M et al. (1978) Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur. J. Biochem. 82 211-7 PubMed
  3. ↑ Chen, R et al. (1980) Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 77 4592-6 PubMed
  4. ↑ Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
  5. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  6. ↑ Molloy, MP et al. (1998) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19 837-44 PubMed
  7. ↑ 7.0 7.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  8. ↑ 8.0 8.1 8.2 CGSC: The Coli Genetics Stock Center
  9. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  10. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  11. ↑ Rowbury, RJ et al. (1992) The PhoE porin and transmission of the chemical stimulus for induction of acid resistance (acid habituation) in Escherichia coli. J. Appl. Bacteriol. 72 233-43 PubMed
  12. ↑ 12.0 12.1 12.2 Santangelo, JD et al. (1991) Metronidazole activation and isolation of Clostridium acetobutylicum electron transport genes. J. Bacteriol. 173 1088-95 PubMed
  13. ↑ 13.0 13.1 13.2 13.3 13.4 13.5 Manoil, C & Rosenbusch, JP (1982) Conjugation-deficient mutants of Escherichia coli distinguish classes of functions of the outer membrane OmpA protein. Mol. Gen. Genet. 187 148-56 PubMed
  14. ↑ Manning, PA & Reeves, P (1976) Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants. J. Bacteriol. 127 1070-9 PubMed
  15. ↑ 15.0 15.1 Manning, PA & Reeves, P (1976) Outer membrane of Escherichia coli K-12: TSX mutants (resistant to bacteriophage T6 and colicin K) lack an outer membrane protein. Biochem. Biophys. Res. Commun. 71 466-71 PubMed
  16. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  17. ↑ 17.0 17.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  18. ↑ 18.0 18.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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