ompA:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
OmpA |
|---|---|
| Synonyms |
outer membrane protein A (3a;II*;G;d)[1], B0957[2][1], Tut[2][1], Con[2][1], TolG[2][1], OmpA[2][1], con, ECK0948, JW0940, tolG, tut, b0957 |
| Product description |
outer membrane protein 3a (II*;G;d)[2][3] Outer membrane protein A; Outer membrane porin A; T-even phage receptor; weak porin; homodimer, abundant cell surface protein[4] Discovered to influence biofilm formation[5] OmpA inhibits cellulose production through the CpxRA stress response system, and this reduction in cellulose increases biofilm formation on hydrophobic surfaces[6]. Deletion of ompA increases cellulose which decreases biofilm formation on hydrophobic surfaces[6]. |
| EC number (for enzymes) |
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| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000746 |
conjugation |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005198 |
structural molecule activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005515 |
protein binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0005515 |
protein binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009279 |
cell outer membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0006811 |
ion transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0019867 |
outer membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0009279 |
cell outer membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015288 |
porin activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0009279 |
cell outer membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0009279 |
cell outer membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006810 |
transport |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0009279 |
cell outer membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009279 |
cell outer membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046718 |
entry of virus into host cell |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0000746 |
conjugation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0009279 |
cell outer membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009597 |
detection of virus |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0006811 |
ion transport |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0019867 |
outer membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0009279 |
cell outer membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009279 |
cell outer membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0009597 |
detection of virus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005515 |
protein binding |
IDA: Inferred from Direct Assay |
F |
with TolB |
complete | |||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009279 |
cell outer membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046718 |
entry of virus into host cell |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046930 |
pore complex |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006974 |
response to DNA damage stimulus |
IEP: Inferred from Expression Pattern |
P |
complete | ||||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
nuoC |
MALDI(Z-score):25.885148 | ||
|
Protein |
rplI |
LCMS(ID Probability):99.6 | ||
|
Protein |
clpB |
LCMS(ID Probability):99.3 | ||
|
Protein |
pflB |
MALDI(Z-score):21.041644 | ||
|
Protein |
tig |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplW |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsN |
LCMS(ID Probability):99.6 | ||
|
Protein |
Hfq |
Hfq stimulates ompA mRNA degradation in a growth rate-dependent manner |
PMID 9826663 |
Hfq interferes with ribosome binding, exposing mRNA to endonuclease cleavage |
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes | |
|---|---|---|---|---|---|
|
outer membrane |
From EcoCyc[3] |
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|
Outer Membrane |
PMID:340230[18], PMID:7001461[19], PMID:8740179[20], PMID:9298646[21], PMID:9629924[22]. |
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|
Outer Membrane |
PMID:9298646 |
| |||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MKKTAIAIAV ALAGFATVAQ AAPKDNTWYT GAKLGWSQYH DTGFINNNGP THENQLGAGA FGGYQVNPYV GFEMGYDWLG RMPYKGSVEN GAYKAQGVQL TAKLGYPITD DLDIYTRLGG MVWRADTKSN VYGKNHDTGV SPVFAGGVEY AITPEIATRL EYQWTNNIGD AHTIGTRPDN GMLSLGVSYR FGQGEAAPVV APAPAPAPEV QTKHFTLKSD VLFNFNKATL KPEGQAALDQ LYSQLSNLDP KDGSVVVLGY TDRIGSDAYN QGLSERRAQS VVDYLISKGI PADKISARGM GESNPVTGNT CDNVKQRAAL IDCLAPDRRV EIEVKGIKDV VTQPQA |
| Length |
346 |
| Mol. Wt |
37.2 kDa |
| pI |
6.4 (calculated) |
| Extinction coefficient |
52,830 - 53,080 (calc based on 17 Y, 5 W, and 2 C residues) |
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Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Barrios, AF et al. (2006) Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility. Biotechnol. Bioeng. 93 188-200 PubMed
- ↑ 6.0 6.1 Ma, Q & Wood, TK (2009) OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two-component system. Environ. Microbiol. 11 2735-46 PubMed
- ↑ 7.0 7.1 Clavel, T et al. (1998) TolB protein of Escherichia coli K-12 interacts with the outer membrane peptidoglycan-associated proteins Pal, Lpp and OmpA. Mol. Microbiol. 29 359-67 PubMed
- ↑ 8.0 8.1 8.2 8.3 8.4 Bulieris, PV et al. (2003) Folding and insertion of the outer membrane protein OmpA is assisted by the chaperone Skp and by lipopolysaccharide. J. Biol. Chem. 278 9092-9 PubMed
- ↑ 9.0 9.1 9.2 Arora, A et al. (2000) Refolded outer membrane protein A of Escherichia coli forms ion channels with two conductance states in planar lipid bilayers. J. Biol. Chem. 275 1594-600 PubMed
- ↑ Klose, M et al. (1988) The influence of amino substitutions within the mature part of an Escherichia coli outer membrane protein (OmpA) on assembly of the polypeptide into its membrane. J. Biol. Chem. 263 13297-302 PubMed
- ↑ 11.0 11.1 Henning, U et al. (1978) Mutants (ompA) affecting a major outer membrane protein of Escherichia coli K12. Eur. J. Biochem. 92 491-8 PubMed
- ↑ Ried, G & Henning, U (1987) A unique amino acid substitution in the outer membrane protein OmpA causes conjugation deficiency in Escherichia coli K-12. FEBS Lett. 223 387-90 PubMed
- ↑ 13.0 13.1 Klose, M et al. (1988) Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptide. J. Biol. Chem. 263 13291-6 PubMed
- ↑ Arutyunov, D et al. (2010) F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation. J. Bacteriol. 192 1730-4 PubMed
- ↑ Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed
- ↑ 16.0 16.1 16.2 16.3 16.4 16.5 16.6 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Zhang, A et al. (2002) The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs. Mol. Cell 9 11-22 PubMed
- ↑ Schweizer, M et al. (1978) Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur. J. Biochem. 82 211-7 PubMed
- ↑ Chen, R et al. (1980) Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 77 4592-6 PubMed
- ↑ Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Molloy, MP et al. (1998) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19 837-44 PubMed
- ↑ 23.0 23.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000746 ! conjugation
- GO:0005198 ! structural molecule activity
- GO:0005515 ! protein binding
- GO:0005886 ! plasma membrane
- GO:0016020 ! membrane
- GO:0006810 ! transport
- GO:0009279 ! cell outer membrane
- GO:0006811 ! ion transport
- GO:0019867 ! outer membrane
- GO:0015288 ! porin activity
- GO:0016021 ! integral component of membrane
- GO:0046718 ! viral entry into host cell
- GO:0009597 ! detection of virus
- GO:0046930 ! pore complex
- GO:0006974 ! cellular response to DNA damage stimulus
- Proteins
