ompA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ompA |
|---|---|
| Mnemonic |
Outer membrane protein |
| Synonyms |
ECK0948, b0957, JW0940, con, tolG, tut[1], tut |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
21.95 minutes |
MG1655: 1019276..1018236 |
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NC_012967: 1037144..1036104 |
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NC_012759: 921204..922244 |
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W3110 |
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W3110: 1020475..1019435 |
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DH10B: 1073204..1072164 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1018239 |
Edman degradation |
PMID:340230[2] |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ompA(del) (Keio:JW0940) |
deletion |
deletion |
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ompA::Tn5KAN-2 (FB20072) |
Insertion at nt 518 in Minus orientation |
does not contain pKD46 | |||||
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ompA::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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ompA252 |
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ompA256 |
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ompA772(del)::kan |
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P678-54ompA2101 |
Sensitivity to |
increased sensitivity to acid, table 3. |
Experimental strain: RJR200 |
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ompA in strain P460 |
Resistant to |
Resistant to bacteriocin Colicin K |
Strain: P460 |
table 3 | |||
|
ompA in strain P1636 |
Resistant to |
Resistant to bacteriocin Colicin L |
Strain: P460 |
table 3 | |||
|
ompA in strain P460 |
Resistant to |
Resistant to bacteriophage K3 |
Strain: p460 |
Figure 6 | |||
|
ompA1 |
Resistant to |
Resistant to novobicin |
Strains: ompA1, ompA2 and ompA3 |
table 6 | |||
|
ompA1 |
Resistant to |
Resistant to chelator, specifically EDTA |
Strains: ompA1, ompA2 and ompA3 |
table 6 | |||
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ompA in strain CC205 |
Resistant to |
Resistant to Phage TUII* |
Strain: CC205 |
Table 4 | |||
|
ompA in strain CC205 |
Resistant to |
Resistant to phage K3hl |
strain CC205 |
Table 4 | |||
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ompA in strain CC205 |
Resistant to |
Resistant to pphage K3 |
Strain: cc205 |
table 4 | |||
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ompA in strain P460 |
Resistant to |
Resistance to Bacteriocin JF246 |
Strain P460 |
Table 3 | |||
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con-tsx in P1731 |
Resistant to |
Resistance to Bacteriophage T6 |
Double Mutation to the con & tsx genes cause resistance to the phage T6, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. | ||||
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con-tsx in P1731 |
Resistant to |
Resistance to Colicin K |
Double Mutation to the con & tsx genes cause resistance to the Colicin K, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0940 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAAAGACAGCTATCGCGAT Primer 2:CCAGCCTGCGGCTGAGTTACAA | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 57% [18] | ||
|
Linked marker |
est. P1 cotransduction: 14% [18] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Schweizer, M et al. (1978) Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur. J. Biochem. 82 211-7 PubMed
- ↑ Chen, R et al. (1980) Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 77 4592-6 PubMed
- ↑ Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Molloy, MP et al. (1998) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19 837-44 PubMed
- ↑ 7.0 7.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 8.0 8.1 8.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Rowbury, RJ et al. (1992) The PhoE porin and transmission of the chemical stimulus for induction of acid resistance (acid habituation) in Escherichia coli. J. Appl. Bacteriol. 72 233-43 PubMed
- ↑ 12.0 12.1 12.2 Santangelo, JD et al. (1991) Metronidazole activation and isolation of Clostridium acetobutylicum electron transport genes. J. Bacteriol. 173 1088-95 PubMed
- ↑ 13.0 13.1 13.2 13.3 13.4 13.5 Manoil, C & Rosenbusch, JP (1982) Conjugation-deficient mutants of Escherichia coli distinguish classes of functions of the outer membrane OmpA protein. Mol. Gen. Genet. 187 148-56 PubMed
- ↑ Manning, PA & Reeves, P (1976) Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants. J. Bacteriol. 127 1070-9 PubMed
- ↑ 15.0 15.1 Manning, PA & Reeves, P (1976) Outer membrane of Escherichia coli K-12: TSX mutants (resistant to bacteriophage T6 and colicin K) lack an outer membrane protein. Biochem. Biophys. Res. Commun. 71 466-71 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 17.0 17.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 18.0 18.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
