mutH:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{|{{ | {| id="N46a9e75321308" class=" tableEdit Gene_nomenclature_table" | ||
|- | |||
!align=left {{table_heading_style}} |Standard name | |||
|| | |||
''mutH'' | |||
|- | |- | ||
!{{table_heading_style}} | | !align=left {{table_heading_style}} |Mnemonic | ||
| | || | ||
Mutator | |||
|- | |- | ||
!{{table_heading_style}} | | !align=left {{table_heading_style}} |Synonyms | ||
| | || | ||
''ECK2827, b2831, JW2799, mutR, prv''<ref name='LIB:Riley_2006'/>, prv'' | |||
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===Notes=== | ===Notes=== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J46a9e753a0cc0" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
|- | |- | ||
| | | | ||
MG1655 | MG1655 | ||
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63.96 minutes | 63.96 minutes | ||
| | | | ||
MG1655: 2967684..2968373<br /> | MG1655: 2967684..2968373<br /><gbrowseImage> | ||
name=NC_000913:2967684..2968373 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10624 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10624 EcoCyc] | ||
{{GBrowseLink|MG1655|NC_000913|2966684|2969373|GBrowse(MG1655)}} | |||
| | | | ||
{{GBrowseFastaLink|MG1655|NC_000913|2967684|2968373|FASTA Dump}} | |||
*[http://biocyc.org/ECOLI/sequence?object=EG10624 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10624 EcoCyc Sequence Dump] | ||
|- | |- | ||
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[[REL606]] | |||
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NC_012967: 2864282..2864971<br /><gbrowseImage> | |||
name=NC_012967:2864282..2864971 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink|REL606|NC_012967|2864282|2864971|GBrowse (REL606)}} | |||
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{{GBrowseFastaLink|REL606|NC_012967|2864282|2864971|FASTA Dump}} | |||
|- | |||
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[[BW2952]] | |||
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NC_012759: 2854832..2855521<br /><gbrowseImage> | |||
name=NC_012759:2854832..2855521 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|2854832|2855521|GBrowse (BW2952)}} | |||
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{{GBrowseFastaLink|BW2952|NC_012759|2854832|2855521|FASTA Dump}} | |||
|- | |||
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W3110 | W3110 | ||
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W3110: 2968318..2969007<br /> | W3110: 2968318..2969007<br /><gbrowseImage> | ||
name=NC_007779:2968318..2969007 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink|W3110|NC_007779|2967318|2970007|GBrowse(W3110)}} | |||
| | | | ||
{{GBrowseFastaLink|W3110|NC_007779|2968318|2969007|FASTA Dump}} | |||
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[[DH10B]] | |||
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DH10B: 3061554..3062243<br /><gbrowseImage> | |||
name=NC_010473:3061554..3062243 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|DH10B|NC_010473|3060176|3063621|GBrowse(DH10B)}} | |||
| | |||
{{GBrowseFastaLink|DH10B|NC_010473|3060176|3063621|FASTA Dump}} | |||
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===Notes=== | ===Notes=== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y46a9e7545c9d6" class=" tableEdit Gene_sequence_features_table" | ||
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!Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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Coding Start (SO:0000323) | |||
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[[MG1655]] | |||
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2967687 | |||
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Edman degradation | |||
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PMID:3031619<br /> | |||
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<protect><!--Sequence_notes--></protect> | <protect><!--Sequence_notes--></protect> | ||
====Notes==== | ====Notes==== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F46a9e754a3b82" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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PMID:16738554 | |||
| | | | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108467 CGSC10186]<ref name='LIB:cgsc'/> | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108467 CGSC10186]<ref name='LIB:cgsc'/> | ||
| | | | ||
|- | |||
| | |||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009287 mutH::Tn5KAN-I-SceI] (FB20953) | |||
| | |||
Insertion at nt 200 in Minus orientation | |||
| | |||
| | |||
| | |||
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PMID:15262929 | |||
| | |||
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20953] | |||
| | |||
contains [[pKD46]] | |||
|- | |||
| | |||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009287 mutH::Tn5KAN-I-SceI] (FB20954) | |||
| | |||
Insertion at nt 200 in Minus orientation | |||
| | |||
| | |||
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PMID:15262929 | |||
| | |||
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20954] | |||
| | |||
does not contain [[pKD46]] | |||
|- | |||
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mutHQ135K | |||
| | |||
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Q135K | |||
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| | |||
(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64) | |||
| | |||
| | |||
| | |||
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
| | |||
mutHG64R | |||
| | |||
| | |||
G64R | |||
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(in strain: ECOR 50) | |||
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| | |||
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
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mutHD157N | |||
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D157N | |||
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| | |||
In TS28; temperature-sensitive | |||
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| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
| | |||
mutHQ153R | |||
| | |||
| | |||
Q153R | |||
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| | |||
(in strain: ECOR 67) | |||
| | |||
| | |||
| | |||
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
| | |||
mutHR155C | |||
| | |||
| | |||
R155C | |||
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(in strain: ECOR 35) | |||
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| | |||
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
| | |||
mutHT28L | |||
| | |||
| | |||
T28L | |||
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| | |||
In TS7; temperature-sensitive | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
| | |||
mutHW58S | |||
| | |||
| | |||
W58S | |||
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| | |||
(in strain: ECOR 67) | |||
| | |||
| | |||
| | |||
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |||
| | |||
mutHG60C | |||
| | |||
| | |||
G60C | |||
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| | |||
(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70) | |||
| | |||
| | |||
| | |||
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722] | |||
|- | |- | ||
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PMID:1092652 | |||
| | | | ||
[[cgscmutation:12133|CGSC:12133]] | [[cgscmutation:12133|CGSC:12133]] | ||
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PMID:1649454 | |||
| | | | ||
[[cgscmutation:38814|CGSC:38814]] | [[cgscmutation:38814|CGSC:38814]] | ||
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PMID:16738554 | |||
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[[cgscmutation:101972|CGSC:101972]] | [[cgscmutation:101972|CGSC:101972]] | ||
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<protect><!--Alleles_notes--></protect> | <protect><!--Alleles_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P4e04bfc706e1b" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a9e7555f2f1" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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JW2799 | JW2799 | ||
| Line 248: | Line 517: | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | ||
| | | | ||
PMID:16769691 | |||
Status:Clone OK | Status:Clone OK | ||
| Line 255: | Line 524: | ||
Primer 2:CCtTGGATCAGAAAATGACGGGC | Primer 2:CCtTGGATCAGAAAATGACGGGC | ||
|- | |- | ||
| | |||
[[:Category:Kohara_Phage:5A10|5A10]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=461 Genobase] | |||
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PMID:3038334 | |||
|- | |||
| | |||
[[:Category:Kohara_Phage:8H3|8H3]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=462 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |||
| | | | ||
[[recD:Gene|recD1901::Tn10]] | [[recD:Gene|recD1901::Tn10]] | ||
| Line 264: | Line 551: | ||
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est. P1 cotransduction: 55% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 55% <ref name='LIB:Nichols_linked_Tn'/> | ||
|- | |- | ||
| | | | ||
[[speA:Gene|speA210::Tn10]] | [[speA:Gene|speA210::Tn10]] | ||
| Line 272: | Line 559: | ||
CAG12168 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40110 CGSC7431]<ref name='LIB:cgsc'/> | CAG12168 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40110 CGSC7431]<ref name='LIB:cgsc'/> | ||
| | | | ||
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:zgd-210::Tn10, zgf-210::Tn10 | |||
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<protect><!--Gene_resources_notes--></protect> | <protect><!--Gene_resources_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9e7560353c" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[[EcoCyc]] | |||
| | | | ||
EcoCyc:EG10624 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG10624 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120000617 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=947299 GeneID:947299] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0619 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0009287 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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===Categories=== | ===Categories=== | ||
[[Category:Gene List:MG1655]] | [[Category:Gene List:MG1655]] | ||
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[[Category:Genes in the Keio knockout collection]] | [[Category:Genes in the Keio knockout collection]] | ||
[[Category:Genes in the ASKA clone collection]] | [[Category:Genes in the ASKA clone collection]] | ||
[[Category:mut genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | |||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:44, 19 September 2013
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
mutH |
|---|---|
| Mnemonic |
Mutator |
| Synonyms |
ECK2827, b2831, JW2799, mutR, prv[1], prv |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
63.96 minutes |
MG1655: 2967684..2968373 |
||
|
NC_012967: 2864282..2864971 |
||||
|
NC_012759: 2854832..2855521 |
||||
|
W3110 |
|
W3110: 2968318..2969007 |
||
|
DH10B: 3061554..3062243 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2967687 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔmutH (Keio:JW2799) |
deletion |
deletion |
|||||
|
mutH::Tn5KAN-I-SceI (FB20953) |
Insertion at nt 200 in Minus orientation |
contains pKD46 | |||||
|
mutH::Tn5KAN-I-SceI (FB20954) |
Insertion at nt 200 in Minus orientation |
does not contain pKD46 | |||||
|
mutHQ135K |
Q135K |
(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHG64R |
G64R |
(in strain: ECOR 50) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHD157N |
D157N |
In TS28; temperature-sensitive |
seeded from UniProt:P06722 | ||||
|
mutHQ153R |
Q153R |
(in strain: ECOR 67) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHR155C |
R155C |
(in strain: ECOR 35) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHT28L |
T28L |
In TS7; temperature-sensitive |
seeded from UniProt:P06722 | ||||
|
mutHW58S |
W58S |
(in strain: ECOR 67) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutHG60C |
G60C |
(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70) |
Strain variation; seeded from UniProt:P06722 | ||||
|
mutH34 |
|||||||
|
mutH471::kan |
|||||||
|
mutH472::mini-Tn10 |
|||||||
|
ΔmutH756::kan |
| ||||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2799 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCCCAACCTCGCCCACTGCT Primer 2:CCtTGGATCAGAAAATGACGGGC | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 55% [10] | ||
|
Linked marker |
est. P1 cotransduction: % [10] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Grafstrom, RH & Hoess, RH (1987) Nucleotide sequence of the Escherichia coli mutH gene. Nucleic Acids Res. 15 3073-84 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Hoess, RH & Herman, RK (1975) Isolation and characterization of mutator strains of Escherichia coli K-12. J. Bacteriol. 122 474-84 PubMed
- ↑ Michaels, ML et al. (1991) MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19 3629-32 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).