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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
|}
[[Category:Gene List:MG1655]][[Category:Gene List:W3110]][[Category:Genes in the Keio knockout collection]][[Category:Genes in the ASKA clone collection]]
</noinclude><protect>
</noinclude><protect>
==Nomenclature==
==Nomenclature==
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|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
|
||
''mutH''  
''mutH''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Mutator
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK2827, b2831, JW2799, mutR, prv''<ref name='LIB:Riley_2006'/>, prv''


|-
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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16229.N46a9e75321308&page=16229&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK2827, b2831, JW2799, mutR, prv''<ref name='LIB:Riley_2006'/>
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===Notes===
===Notes===
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<protect>
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J46a9e753a0cc0"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
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MG1655
MG1655
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63.96 minutes&nbsp;
63.96 minutes&nbsp;
|
|
MG1655: 2967684..2968373<br />{{GBrowseFigureByCoord|2966684|2969373|ecoli|MG1655}}
MG1655: 2967684..2968373<br /><gbrowseImage>
name=NC_000913:2967684..2968373
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10624 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10624 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:2966684..2969373 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|2966684|2969373|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:2967684..2968373&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|2967684|2968373|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10624 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10624 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 2864282..2864971<br /><gbrowseImage>
name=NC_012967:2864282..2864971
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|2864282|2864971|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2864282|2864971|FASTA Dump}}
|-
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[[BW2952]]
|
 
|
NC_012759: 2854832..2855521<br /><gbrowseImage>
name=NC_012759:2854832..2855521
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|2854832|2855521|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2854832|2855521|FASTA Dump}}
|-  
|
|
W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 2968318..2969007<br />{{GBrowseFigureByCoord|2967318|2970007|ecoli_W3110|W3110}}
W3110: 2968318..2969007<br /><gbrowseImage>
name=NC_007779:2968318..2969007
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:2967318..2970007 GBrowse(W3110)]
{{GBrowseLink|W3110|NC_007779|2967318|2970007|GBrowse(W3110)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:2968318..2969007&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|W3110|NC_007779|2968318|2969007|FASTA Dump}}
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[[DH10B]]
|
 
|
DH10B: 3061554..3062243<br /><gbrowseImage>
name=NC_010473:3061554..3062243
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|3060176|3063621|GBrowse(DH10B)}}
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{{GBrowseFastaLink|DH10B|NC_010473|3060176|3063621|FASTA Dump}}
 
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
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[[MG1655]]
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2967687
|
Edman degradation
|
PMID:3031619<br />
|
 
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="F46a9e754a3b82"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
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&Delta;mutH (Keio:JW2799)
&Delta;mutH (Keio:JW2799)
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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108467 CGSC10186]<ref name='LIB:cgsc'/>
|
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009287 mutH::Tn5KAN-I-SceI] (FB20953)
|
Insertion at nt 200 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20953]
|
contains [[pKD46]]
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009287 mutH::Tn5KAN-I-SceI] (FB20954)
|
Insertion at nt 200 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20954]
|
does not contain [[pKD46]]
|-
|
mutHQ135K
|
 
|
Q135K
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|
(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64)
|
 
|
 
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
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mutHG64R
|
 
|
G64R
|
 
|
(in strain: ECOR 50)
|
 
|
 
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
mutHD157N
|
 
|
D157N
|
 
|
In TS28; temperature-sensitive
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
mutHQ153R
|
 
|
Q153R
|
 
|
(in strain: ECOR 67)
|
 
|
 
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
mutHR155C
|
 
|
R155C
|
 
|
(in strain: ECOR 35)
|
 
|
 
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
mutHT28L
|
 
|
T28L
|
 
|
In TS7; temperature-sensitive
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
mutHW58S
|
 
|
W58S
|
 
|
(in strain: ECOR 67)
|
 
|
 
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
mutHG60C
|
 
|
G60C
|
 
|
(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70)
|
 
|
 
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P06722 UniProt:P06722]
|-
|
''mutH34''
|
 
|
 
|
 
|
 
|
PMID:1092652
|
[[cgscmutation:12133|CGSC:12133]]
|
|-
|
''mutH471::kan''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:30821|CGSC:30821]]
|
|-
|
''mutH472::mini-Tn10''
|
 
|
 
|
 
|
 
|
PMID:1649454
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]  
[[cgscmutation:38814|CGSC:38814]]
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''&#916;mutH756::kan''
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PMID:16738554
|
[[cgscmutation:101972|CGSC:101972]]
|
 
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===Notes===
===Notes===


==Genetic Interactions==
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|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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|- {{table_heading_style}}
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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JW2799
JW2799
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 160: Line 524:


Primer 2:CCtTGGATCAGAAAATGACGGGC  
Primer 2:CCtTGGATCAGAAAATGACGGGC  
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[[:Category:Kohara_Phage:5A10|5A10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=461 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:8H3|8H3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=462 Genobase]
|
PMID:3038334
|-
|
[[recD:Gene|recD1901::Tn10]]
|
Linked marker
|
CAG12135 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40106 CGSC7429]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 55% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[speA:Gene|speA210::Tn10]]
|
Linked marker
|
CAG12168 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40110 CGSC7431]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zgd-210::Tn10, zgf-210::Tn10
 
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===Notes===
===Notes===
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10624 EcoCyc:EG10624] 
EcoCyc:EG10624
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0619 EchoBASE:EB0619] 
EcoGene:EG10624
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b2831 EcoliGenExpDB:b2831] 
RegulonDB:ECK120000617
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C3426 ECOL199310CYC:C3426]
[http://www.ncbi.nlm.nih.gov/gene?term=947299 GeneID:947299]
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Escherichia coli str. K-12 substr. MG1655
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EchoBASE]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10624 EcoGene:EG10624] 
EchoBASE:EB0619
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[ASAP]]
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[http://BioCyc.org/ECOO157/new-image?object=MUTH ECOO157CYC:MUTH] 
ASAP:ABE-0009287
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/471 CGSC:471] 
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===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:mut genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:44, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mutH

Mnemonic

Mutator

Synonyms

ECK2827, b2831, JW2799, mutR, prv[1], prv

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.96 minutes 

MG1655: 2967684..2968373
<gbrowseImage> name=NC_000913:2967684..2968373 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2864282..2864971
<gbrowseImage> name=NC_012967:2864282..2864971 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2854832..2855521
<gbrowseImage> name=NC_012759:2854832..2855521 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2968318..2969007
<gbrowseImage> name=NC_007779:2968318..2969007 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3061554..3062243
<gbrowseImage> name=NC_010473:3061554..3062243 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2967687

Edman degradation

PMID:3031619[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmutH (Keio:JW2799)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10186[4]

mutH::Tn5KAN-I-SceI (FB20953)

Insertion at nt 200 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20953

contains pKD46

mutH::Tn5KAN-I-SceI (FB20954)

Insertion at nt 200 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20954

does not contain pKD46

mutHQ135K

Q135K

(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64)

Strain variation; seeded from UniProt:P06722

mutHG64R

G64R

(in strain: ECOR 50)

Strain variation; seeded from UniProt:P06722

mutHD157N

D157N

In TS28; temperature-sensitive

seeded from UniProt:P06722

mutHQ153R

Q153R

(in strain: ECOR 67)

Strain variation; seeded from UniProt:P06722

mutHR155C

R155C

(in strain: ECOR 35)

Strain variation; seeded from UniProt:P06722

mutHT28L

T28L

In TS7; temperature-sensitive

seeded from UniProt:P06722

mutHW58S

W58S

(in strain: ECOR 67)

Strain variation; seeded from UniProt:P06722

mutHG60C

G60C

(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70)

Strain variation; seeded from UniProt:P06722

mutH34

PMID:1092652[6]

CGSC:12133

mutH471::kan

CGSC:30821

mutH472::mini-Tn10

PMID:1649454[7]

CGSC:38814

ΔmutH756::kan

PMID:16738554[3]

CGSC:101972


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2799

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTCCCAACCTCGCCCACTGCT

Primer 2:CCtTGGATCAGAAAATGACGGGC

5A10

Kohara Phage

Genobase

PMID:3038334[9]

8H3

Kohara Phage

Genobase

PMID:3038334[9]

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[4]

est. P1 cotransduction: 55% [10]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[4]

est. P1 cotransduction: % [10]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10624

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10624

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000617

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947299

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0619

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009287

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Grafstrom, RH & Hoess, RH (1987) Nucleotide sequence of the Escherichia coli mutH gene. Nucleic Acids Res. 15 3073-84 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Hoess, RH & Herman, RK (1975) Isolation and characterization of mutator strains of Escherichia coli K-12. J. Bacteriol. 122 474-84 PubMed
  7. ↑ Michaels, ML et al. (1991) MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19 3629-32 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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