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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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W3110: 3728315..3729088<br /><gbrowseImage>
W3110: 3728315..3729088<br /><gbrowseImage>
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{{GBrowseLink|W3110|AC_000091|3727315|3730088|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|3727315|3730088|GBrowse(W3110)}}  
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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&Delta;pstB (Keio:JW3703)
''pstB(del)'' (Keio:JW3703)
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deletion
deletion
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PMID:16738554
PMID:16738554
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108988 CGSC10707]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=108988 CGSC10707]<ref name='LIB:cgsc'/>  
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&Delta;pstB::kan
''pstB(del)''::kan
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deletion
deletion
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&Delta;pstB::kan
''pstB(del)''::kan
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deletion
deletion
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''&#916;pstB756::kan''
''pstB756(del)''::kan
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Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.  
Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.  
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''pstB756(del)::FRT''
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Sensitivity to
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Increase sensitivity to UV.
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PMID:23224554
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Experimental Strain: SMR12050
Parental strain: SMR4562
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See Table S1 & S7
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CAG45114 ''pstB756(del)''::FRTKanFRT
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Deletion
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Sigma<sup>E</sup> activity
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Decrease in Sigma<sup>E</sup> activity
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PMID:23224554
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Parental Strain: CAG45114
Experimental Strain: SMR15301
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See table S11 for experimental data. 


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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:EG10783
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10783 ECOCYC:EG10783]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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Escherichia coli str. K-12 substr. MG1655  
[[EcoGene]]
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EcoGene:EG10783
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10783 EcoGene:EG10783]
RegulonDB:ECK120000774
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0776
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0012179
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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!|Name!!URL!!Comments
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Latest revision as of 20:49, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pstB

Mnemonic

P-specific transport

Synonyms

ECK3718, b3725, JW3703, phoT[1], phoT

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.18 minutes 

MG1655: 3906389..3905616
<gbrowseImage> name=NC_000913:3905616..3906389 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3868733..3867960
<gbrowseImage> name=NC_012967:3867960..3868733 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3793949..3794722
<gbrowseImage> name=NC_012759:3793949..3794722 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3728315..3729088
<gbrowseImage> name=NC_007779:3728315..3729088 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4003973..4003200
<gbrowseImage> name=NC_010473:4003200..4003973 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

pstB(del) (Keio:JW3703)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10707[3]

pstB::Tn5KAN-I-SceI (FB21418)

Insertion at nt 405 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB21418

contains pKD46

pstB::Tn5KAN-I-SceI (FB21419)

Insertion at nt 405 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB21419

does not contain pKD46

pstBG48I

G48I

Loss of phosphate transport

seeded from UniProt:P0AAH0

pstBK49Q

K49Q

Loss of phosphate transport

seeded from UniProt:P0AAH0

pstB(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[5]

pstB(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[5]

pstB608(del-ins)::kan

PMID:10829079[6]

CGSC:65933

pstB629(del-ins,::FRT)

PMID:10829079[6]

CGSC:65936

pstB756(del)::kan

PMID:16738554[2]

CGSC:102506

ptsB in strain 10B5

Resistant to

Resistant to Arsenate

PMID:765745[7]

Strain: 10B5

pstB in strain UR1

Resistant to

Resistant to Arsenate

PMID:4570598[8]

Strain: UR1

pstB756(del)::FRT

Mutagenesis rate

Decreased stress-induced mutagenesis (SIM) phenotype.

PMID:23224554[9]

Parental Strain: SMR4562

Experimental Strain: SMR12232

Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.

pstB756(del)::FRT

Sensitivity to

Increase sensitivity to UV.

PMID:23224554[9]

Experimental Strain: SMR12050

Parental strain: SMR4562

See Table S1 & S7

CAG45114 pstB756(del)::FRTKanFRT

Deletion

SigmaE activity

Decrease in SigmaE activity

PMID:23224554[9]

Parental Strain: CAG45114 Experimental Strain: SMR15301

See table S11 for experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3703

Plasmid clone

Shigen

PMID:16769691[10]

Status:Clone OK

Primer 1:GCCAGTATGGTTGAAACTGCCCC

Primer 2:CCACCGTAACGACCGGTGATGTA

7A1

Kohara Phage

Genobase

PMID:3038334[11]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[3]

est. P1 cotransduction: 29% [12]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[3]

est. P1 cotransduction: 40% [12]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10783

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10783

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000774

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948240

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0776

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012179

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ 5.0 5.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. ↑ 6.0 6.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
  7. ↑ Sprague, GF Jr et al. (1975) A mutant of Escherichia coli auxotrophic for organic phosphates: evidence for two defects in inorganic phosphate transport. Mol. Gen. Genet. 143 71-7 PubMed
  8. ↑ Willsky, GR et al. (1973) Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J. Bacteriol. 113 529-39 PubMed
  9. ↑ 9.0 9.1 9.2 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  10. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  11. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  12. ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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