ompA:Gene: Difference between revisions
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|{{Sub}}[[#Sequence Features|Sequence Features]] | |{{Sub}}[[#Sequence Features|Sequence Features]] | ||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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****************************************************************************************** -->{{Gene_nomenclature_help}} | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| | {| id="B46a9bc7cf1ca3" class=" tableEdit Gene_nomenclature_table" | ||
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!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK0948, b0957, JW0940, con, tolG, tut''<ref name='LIB:Riley_2006'/> | ''ECK0948, b0957, JW0940, con, tolG, tut''<ref name='LIB:Riley_2006'/>, tut'' | ||
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****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46a9bc7d7658c" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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21.95 minutes | 21.95 minutes | ||
| | | | ||
MG1655: 1019276..1018236<br /> | MG1655: 1019276..1018236<br /><gbrowseImage> | ||
name=NC_000913:1018236..1019276 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10669 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10669 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|1017236|1020276|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|1018236|1019276|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10669 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10669 EcoCyc Sequence Dump] | ||
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NC_012967: 1037144..1036104<br /> | NC_012967: 1037144..1036104<br /><gbrowseImage> | ||
name=NC_012967:1036104..1037144 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink|REL606|NC_012967|1036104|1037144|GBrowse (REL606)}} | {{GBrowseLink|REL606|NC_012967|1036104|1037144|GBrowse (REL606)}} | ||
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NC_012759: 921204..922244<br /> | NC_012759: 921204..922244<br /><gbrowseImage> | ||
name=NC_012759:921204..922244 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink|BW2952| | {{GBrowseLink|BW2952|NC_012759|921204|922244|GBrowse (BW2952)}} | ||
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{{GBrowseFastaLink|BW2952| | {{GBrowseFastaLink|BW2952|NC_012759|921204|922244|FASTA Dump}} | ||
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W3110: 1020475..1019435<br /> | W3110: 1020475..1019435<br /><gbrowseImage> | ||
name=NC_007779:1019435..1020475 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|W3110|NC_007779|1018435|1021475|GBrowse(W3110)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|W3110|NC_007779|1019435|1020475|FASTA Dump}} | ||
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DH10B: 1073204..1072164<br /> | DH10B: 1073204..1072164<br /><gbrowseImage> | ||
name=NC_010473:1072164..1073204 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|1070084|1075284|GBrowse(DH10B)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|1070084|1075284|FASTA Dump}} | ||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9bc7e20c70" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46a9bc7e6156d" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|- | |- | ||
| | | | ||
''ompA(del)'' (Keio:JW0940) | |||
| | | | ||
deletion | deletion | ||
| Line 198: | Line 224: | ||
PMID:16738554 | PMID:16738554 | ||
| | | | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | ||
[http://cgsc2.biology.yale.edu/Strain.php?ID=107223 CGSC8942]<ref name='LIB:cgsc'/> | |||
| | | | ||
|- | |- | ||
| | | | ||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003240 ompA] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003240 ompA::Tn5KAN-2] (FB20072) | ||
| | | | ||
Insertion at nt 518 in Minus orientation | |||
| | | | ||
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| | | | ||
PMID: | PMID:15262929 | ||
| | | | ||
[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20072] | ||
| | | | ||
does not contain [[pKD46]] | |||
|- | |- | ||
| | | | ||
''ompA''::kan | |||
| | | | ||
deletion | deletion | ||
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|- | |- | ||
| | | | ||
'' | ''ompA772(del)::kan'' | ||
| | | | ||
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| | | | ||
|- | |||
| | |||
''P678-54ompA2101'' | |||
| | |||
| | |||
| | |||
Sensitivity to | |||
| | |||
increased sensitivity to acid, table 3. | |||
| | |||
PMID:1314797 | |||
| | |||
Experimental strain: RJR200 | |||
| | |||
|- | |||
| | |||
''ompA'' in strain P460 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to bacteriocin Colicin K | |||
| | |||
PMID:1991710 | |||
| | |||
Strain: P460 | |||
| | |||
table 3 | |||
|- | |||
| | |||
''ompA'' in strain P1636 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to bacteriocin Colicin L | |||
| | |||
PMID:1991710 | |||
| | |||
Strain: P460 | |||
| | |||
table 3 | |||
|- | |||
| | |||
''ompA'' in strain P460 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to bacteriophage K3 | |||
| | |||
PMID:1991710 | |||
PMID:6819426 | |||
| | |||
Strain: p460 | |||
| | |||
Figure 6 | |||
|- | |||
| | |||
''ompA1'' | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to novobicin | |||
| | |||
PMID:6819426 | |||
| | |||
Strains: ompA1, ompA2 and ompA3 | |||
| | |||
table 6 | |||
|- | |||
| | |||
''ompA1'' | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to chelator, specifically EDTA | |||
| | |||
PMID:6819426 | |||
| | |||
Strains: ompA1, ompA2 and ompA3 | |||
| | |||
table 6 | |||
|- | |||
| | |||
''ompA'' in strain CC205 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to Phage TUII<nowiki>*</nowiki> | |||
| | |||
PMID:6819426 | |||
| | |||
Strain: CC205 | |||
| | |||
Table 4 | |||
|- | |||
| | |||
''ompA'' in strain CC205 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to phage K3hl | |||
| | |||
PMID:6819426 | |||
| | |||
strain CC205 | |||
| | |||
Table 4 | |||
|- | |||
| | |||
''ompA'' in strain CC205 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistant to pphage K3 | |||
| | |||
PMID:6819426 | |||
| | |||
Strain: cc205 | |||
| | |||
table 4 | |||
|- | |||
| | |||
''ompA'' in strain P460 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistance to Bacteriocin JF246 | |||
| | |||
PMID:783128 | |||
| | |||
Strain P460 | |||
| | |||
Table 3 | |||
|- | |||
| | |||
''con''-''tsx'' in P1731 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistance to Bacteriophage T6 | |||
| | |||
PMID:786287 | |||
| | |||
| | |||
Double Mutation to the ''con'' & ''tsx'' genes cause resistance to the phage T6, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. | |||
|- | |||
| | |||
''con''-''tsx'' in P1731 | |||
| | |||
| | |||
| | |||
Resistant to | |||
| | |||
Resistance to Colicin K | |||
| | |||
PMID:786287 | |||
| | |||
| | |||
Double Mutation to the ''con'' & ''tsx'' genes cause resistance to the Colicin K, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.12473.Q4e04c07a8264c&page=12473&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="V46a9bc7f0bd83" class=" tableEdit Gene_resource_table" | ||
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Primer 2:CCAGCCTGCGGCTGAGTTACAA | Primer 2:CCAGCCTGCGGCTGAGTTACAA | ||
|- | |||
| | |||
[[:Category:Kohara_Phage:E2E5|E2E5]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=222 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |||
| | |||
[[:Category:Kohara_Phage:9B10|9B10]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=223 Genobase] | |||
| | |||
PMID:3038334 | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9bc7f95ebb" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[ | [[EcoCyc]] | ||
[ | |||
| | | | ||
EcoCyc:EG10669 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG10669 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120000662 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=945571 GeneID:945571] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0663 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0003240 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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!|Name!!URL!!Comments | !|Name!!URL!!Comments | ||
Latest revision as of 20:32, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ompA |
|---|---|
| Mnemonic |
Outer membrane protein |
| Synonyms |
ECK0948, b0957, JW0940, con, tolG, tut[1], tut |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
21.95 minutes |
MG1655: 1019276..1018236 |
||
|
NC_012967: 1037144..1036104 |
||||
|
NC_012759: 921204..922244 |
||||
|
W3110 |
|
W3110: 1020475..1019435 |
||
|
DH10B: 1073204..1072164 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1018239 |
Edman degradation |
PMID:340230[2] |
| |
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ompA(del) (Keio:JW0940) |
deletion |
deletion |
|||||
|
ompA::Tn5KAN-2 (FB20072) |
Insertion at nt 518 in Minus orientation |
does not contain pKD46 | |||||
|
ompA::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
||||
|
ompA252 |
|||||||
|
ompA256 |
|||||||
|
ompA772(del)::kan |
|||||||
|
P678-54ompA2101 |
Sensitivity to |
increased sensitivity to acid, table 3. |
Experimental strain: RJR200 |
||||
|
ompA in strain P460 |
Resistant to |
Resistant to bacteriocin Colicin K |
Strain: P460 |
table 3 | |||
|
ompA in strain P1636 |
Resistant to |
Resistant to bacteriocin Colicin L |
Strain: P460 |
table 3 | |||
|
ompA in strain P460 |
Resistant to |
Resistant to bacteriophage K3 |
Strain: p460 |
Figure 6 | |||
|
ompA1 |
Resistant to |
Resistant to novobicin |
Strains: ompA1, ompA2 and ompA3 |
table 6 | |||
|
ompA1 |
Resistant to |
Resistant to chelator, specifically EDTA |
Strains: ompA1, ompA2 and ompA3 |
table 6 | |||
|
ompA in strain CC205 |
Resistant to |
Resistant to Phage TUII* |
Strain: CC205 |
Table 4 | |||
|
ompA in strain CC205 |
Resistant to |
Resistant to phage K3hl |
strain CC205 |
Table 4 | |||
|
ompA in strain CC205 |
Resistant to |
Resistant to pphage K3 |
Strain: cc205 |
table 4 | |||
|
ompA in strain P460 |
Resistant to |
Resistance to Bacteriocin JF246 |
Strain P460 |
Table 3 | |||
|
con-tsx in P1731 |
Resistant to |
Resistance to Bacteriophage T6 |
Double Mutation to the con & tsx genes cause resistance to the phage T6, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. | ||||
|
con-tsx in P1731 |
Resistant to |
Resistance to Colicin K |
Double Mutation to the con & tsx genes cause resistance to the Colicin K, possibly due to the reduced production of a protein (tsx-protein) thought to be involved phage binding to the cell. | ||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0940 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAAAGACAGCTATCGCGAT Primer 2:CCAGCCTGCGGCTGAGTTACAA | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 57% [18] | ||
|
Linked marker |
est. P1 cotransduction: 14% [18] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Schweizer, M et al. (1978) Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur. J. Biochem. 82 211-7 PubMed
- ↑ Chen, R et al. (1980) Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 77 4592-6 PubMed
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- ↑ 18.0 18.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).