recB:Gene: Difference between revisions
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|{{Sub}}[[#Sequence Features|Sequence Features]] | |{{Sub}}[[#Sequence Features|Sequence Features]] | ||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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****************************************************************************************** -->{{Gene_nomenclature_help}} | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| | {| id="F46a9e70c5599e" class=" tableEdit Gene_nomenclature_table" | ||
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!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK2816, b2820, JW2788, rorA, ior''<ref name='LIB:Riley_2006'/> | ''ECK2816, b2820, JW2788, rorA, ior''<ref name='LIB:Riley_2006'/>, ior'' | ||
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****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="L46a9e70cd514e" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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63.59 minutes | 63.59 minutes | ||
| | | | ||
MG1655: 2954025..2950483<br /> | MG1655: 2954025..2950483<br /><gbrowseImage> | ||
name=NC_000913:2950483..2954025 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10824 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10824 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|2949483|2955025|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|2950483|2954025|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10824 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10824 EcoCyc Sequence Dump] | ||
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NC_012967: 2850622..2847080<br /> | NC_012967: 2850622..2847080<br /><gbrowseImage> | ||
name=NC_012967:2847080..2850622 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink|REL606|NC_012967|2847080|2850622|GBrowse (REL606)}} | {{GBrowseLink|REL606|NC_012967|2847080|2850622|GBrowse (REL606)}} | ||
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NC_012759: 2837631..2841173<br /> | NC_012759: 2837631..2841173<br /><gbrowseImage> | ||
name=NC_012759:2837631..2841173 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink|BW2952| | {{GBrowseLink|BW2952|NC_012759|2837631|2841173|GBrowse (BW2952)}} | ||
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{{GBrowseFastaLink|BW2952| | {{GBrowseFastaLink|BW2952|NC_012759|2837631|2841173|FASTA Dump}} | ||
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W3110: 2954659..2951117<br /> | W3110: 2954659..2951117<br /><gbrowseImage> | ||
name=NC_007779:2951117..2954659 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|W3110|NC_007779|2950117|2955659|GBrowse(W3110)}} | ||
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{{GBrowseFastaLink| | {{GBrowseFastaLink|W3110|NC_007779|2951117|2954659|FASTA Dump}} | ||
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DH10B: 3047895..3044353<br /> | DH10B: 3047895..3044353<br /><gbrowseImage> | ||
name=NC_010473:3044353..3047895 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|3037269|3054979|GBrowse(DH10B)}} | ||
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{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|3037269|3054979|FASTA Dump}} | ||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y46a9e70d912fc" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|- | |||
| | |||
Coding Start (SO:0000323) | |||
| | |||
[[MG1655]] | |||
| | |||
2950486 | |||
| | |||
Edman degradation | |||
| | |||
PMID:1618858<br /> | |||
| | |||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="L46a9e70dd82cb" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|- | |- | ||
| | | | ||
''recB(del)'' (Keio:JW2788) | |||
| | | | ||
deletion | deletion | ||
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PMID:16738554 | PMID:16738554 | ||
| | | | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108463 CGSC10182]<ref name='LIB:cgsc'/> | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=108463 CGSC10182]<ref name='LIB:cgsc'/> | ||
| | | | ||
|- | |- | ||
| | | | ||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009249 recB::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009249 recB::Tn5KAN-I-SceI] (FB20945) | ||
| | | | ||
Insertion at nt 2423 in Minus orientation | |||
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PMID: | PMID:15262929 | ||
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[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20945] | ||
| | | | ||
contains [[pKD46]] | |||
|- | |- | ||
| | | | ||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009249 recB::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009249 recB::Tn5KAN-I-SceI] (FB20946) | ||
| | | | ||
Insertion at nt 2423 in Minus orientation | |||
| | | | ||
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PMID: | PMID:15262929 | ||
| | | | ||
[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20946] | ||
| | | | ||
does not contain [[pKD46]] | |||
|- | |- | ||
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'' | ''recB745(del)''::kan | ||
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|- | |||
| | |||
recB in [[MDS42]] | |||
| | |||
deletion | |||
| | |||
| | |||
Sensitivity to | |||
| | |||
increases sensitivity to bicyclomycin | |||
| | |||
PMID:21183718 | |||
| | |||
| | |||
fig. 2 | |||
|- | |||
| | |||
recB | |||
| | |||
deletion | |||
| | |||
| | |||
Sensitivity to | |||
| | |||
decreases sensitivity to [[Bicyclomycin]] | |||
| | |||
PMID:21357484 | |||
| | |||
| | |||
fig 2 | |||
|- | |||
| | |||
''nrdA101 recB(del)'' | |||
| | |||
''recB'' function is deactivated | |||
| | |||
| | |||
Rate of DNA replication | |||
| | |||
When exposed to extreme temperatures the rate of transcription is slightly under restrictive conditions | |||
| | |||
PMID:21441507 | |||
| | |||
| | |||
See Figure 2 | |||
|- | |||
| | |||
''recB268''::Tn''10'' | |||
| | |||
Tn''10'' was inserted into the ''recB'' gene at the allele 268. | |||
| | |||
| | |||
Growth Phenotype | |||
| | |||
the rate of growth was significantly decreased. | |||
| | |||
PMID:9973363 | |||
| | |||
| | |||
See figure 1. The strain background is Hfr3000; called '3000HfrH' in the paper. | |||
|- | |||
| | |||
SMR6039 ''recB(del)''::Kan | |||
| | |||
Deletion | |||
| | |||
| | |||
SOS response | |||
| | |||
Decrease in spontaneous SOS-induction defective | |||
| | |||
PMID:23224554 | |||
| | |||
Parent Strain: SMR6039 | |||
Experimental Strain: SMR16024 | |||
| | |||
The mutation caused a defect in Spontaneous SOS-Induction. See table S7 for a summary of experimental results. | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P4e04c225d976d" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a9e70e938b5" class=" tableEdit Gene_resource_table" | ||
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Primer 2:CCgGCCTCCTCCAGGGTCATACC | Primer 2:CCgGCCTCCTCCAGGGTCATACC | ||
|- | |||
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[[:Category:Kohara_Phage:10B6|10B6]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=459 Genobase] | |||
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PMID:3038334 | |||
|- | |||
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[[:Category:Kohara_Phage:3G11|3G11]] | |||
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Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=460 Genobase] | |||
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PMID:3038334 | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J46a9e70f37d7f" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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[ | [[EcoCyc]] | ||
[ | |||
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EcoCyc:EG10824 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EcoGene]] | |||
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EcoGene:EG10824 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000815 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=947286 GeneID:947286] | ||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EchoBASE]] | |||
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EchoBASE:EB0817 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[ASAP]] | |||
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ASAP:ABE-0009249 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="L46a7035138a9d" class=" tableEdit Links_table" | ||
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!|Name!!URL!!Comments | !|Name!!URL!!Comments | ||
Latest revision as of 20:44, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
recB |
|---|---|
| Mnemonic |
Recombination |
| Synonyms |
ECK2816, b2820, JW2788, rorA, ior[1], ior |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
63.59 minutes |
MG1655: 2954025..2950483 |
||
|
NC_012967: 2850622..2847080 |
||||
|
NC_012759: 2837631..2841173 |
||||
|
W3110 |
|
W3110: 2954659..2951117 |
||
|
DH10B: 3047895..3044353 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2950486 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
recB(del) (Keio:JW2788) |
deletion |
deletion |
|||||
|
recB::Tn5KAN-I-SceI (FB20945) |
Insertion at nt 2423 in Minus orientation |
contains pKD46 | |||||
|
recB::Tn5KAN-I-SceI (FB20946) |
Insertion at nt 2423 in Minus orientation |
does not contain pKD46 | |||||
|
recB21 |
|||||||
|
recB270(ts) |
temperature sensitive |
||||||
|
recB1067(D1067A) |
|||||||
|
recB1080(D1080A) |
|||||||
|
recB1082(K1082Q) |
|||||||
|
recB60 |
|||||||
|
recB745(del)::kan |
|||||||
|
recB in MDS42 |
deletion |
Sensitivity to |
increases sensitivity to bicyclomycin |
fig. 2 | |||
|
recB |
deletion |
Sensitivity to |
decreases sensitivity to Bicyclomycin |
fig 2 | |||
|
nrdA101 recB(del) |
recB function is deactivated |
Rate of DNA replication |
When exposed to extreme temperatures the rate of transcription is slightly under restrictive conditions |
See Figure 2 | |||
|
recB268::Tn10 |
Tn10 was inserted into the recB gene at the allele 268. |
Growth Phenotype |
the rate of growth was significantly decreased. |
See figure 1. The strain background is Hfr3000; called '3000HfrH' in the paper. | |||
|
SMR6039 recB(del)::Kan |
Deletion |
SOS response |
Decrease in spontaneous SOS-induction defective |
Parent Strain: SMR6039 Experimental Strain: SMR16024 |
The mutation caused a defect in Spontaneous SOS-Induction. See table S7 for a summary of experimental results. | ||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2788 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGTGATGTCGCCGAGACACT Primer 2:CCgGCCTCCTCCAGGGTCATACC | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 52% [15] | ||
|
Linked marker |
est. P1 cotransduction: 99% [15] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Masterson, C et al. (1992) Reconstitution of the activities of the RecBCD holoenzyme of Escherichia coli from the purified subunits. J. Biol. Chem. 267 13564-72 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 Yu, M et al. (1998) Identification of the nuclease active site in the multifunctional RecBCD enzyme by creation of a chimeric enzyme. J. Mol. Biol. 283 797-808 PubMed
- ↑ Jockovich, ME & Myers, RS (2001) Nuclease activity is essential for RecBCD recombination in Escherichia coli. Mol. Microbiol. 41 949-62 PubMed
- ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
- ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
- ↑ Salguero, I et al. (2011) RecA-dependent replication in the nrdA101(Ts) mutant of Escherichia coli under restrictive conditions. J. Bacteriol. 193 2851-60 PubMed
- ↑ Dermić, D & Trgovcević, Z (1999) Specific effects of a recB mutation on the HfrH strain of Escherichia coli. J. Bacteriol. 181 1334-7 PubMed
- ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 14.0 14.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 15.0 15.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).