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Standard Name	yjbH
Gene Synonym(s)	ECK4021, b4029, JW3989^[1], JW3989
Product Desc.	conserved protein, putative lipoprotein and/or outer membrane porin^[2]; predicted porin^[3] Extracellular polysaccharide production^[4]
Product Synonyms(s)	predicted porin^[1], B4029^[2]^[1], YjbH^[2]^[1] , ECK4021, JW3989, b4029
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yjbF^[2], yjbEFGH^[3]
Regulation/Activity
Quick Links
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Notes

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Overexpression of the yjbEFGH operon alters colony morphology and leads to production of a novel extracellular polysaccharide (Ferrieres, 2007). yjbH and gfcD are paralogs. YjbH has both type II (18 aa) and (somewhat better) type I (21 aa) signal peptide predictions by LipoP. YjbH is not likely to be a lipoprotein because the lipobox Cys is poorly conserved.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yjbH
Mnemonic	Systematic nomenclature
Synonyms	ECK4021, b4029, JW3989^[1], JW3989
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	91.29 minutes, 91.29 minutes	MG1655: 4235657..4237753 <gbrowseImage> name=NC_000913:4235657..4237753 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4216566..4218662 <gbrowseImage> name=NC_012967:4216566..4218662 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4125337..4127433 <gbrowseImage> name=NC_012759:4125337..4127433 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4241224..4243320 <gbrowseImage> name=NC_007779:4241224..4243320 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4335353..4337449 <gbrowseImage> name=NC_010473:4335353..4337449 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔyjbH (Keio:JW3989)	deletion	deletion	PMID:16738554	Shigen CGSC10870^[5]
ΔyjbH725::kan			PMID:16738554	CGSC:105458
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3989	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAAAAAGACATCTGCTTAG Primer 2:CCGCGGAAATTGACGCTACGGTC
7A4	Kohara Phage	Genobase	PMID:3038334
thiC39::Tn10	Linked marker	CAG18500 = CGSC7472^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:thi-39::Tn10
malF3089::Tn10	Linked marker	CAG12164 = CGSC7476^[5]	est. P1 cotransduction: 85% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11926	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11926	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001854	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948527	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1870	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013176	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YjbH
Synonyms	predicted porin^[1], B4029^[2]^[1], YjbH^[2]^[1] , ECK4021, JW3989, b4029
Product description	conserved protein, putative lipoprotein and/or outer membrane porin^[2]; predicted porin^[3] Extracellular polysaccharide production^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplA	PMID:19402753	LCMS(ID Probability):99.6
Protein	clpB	PMID:19402753	LCMS(ID Probability):99.6
Protein	adhE	PMID:19402753	LCMS(ID Probability):99.6
Protein	yjgF	PMID:19402753	MALDI(Z-score):17.252487
Protein	yejK	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplI	PMID:19402753	LCMS(ID Probability):99.6
Protein	uspG	PMID:19402753	LCMS(ID Probability):99.6
Protein	mreB	PMID:19402753	LCMS(ID Probability):99.6
Protein	argG	PMID:19402753	LCMS(ID Probability):99.4
Protein	allC	PMID:19402753	LCMS(ID Probability):98.5
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKKRHLLSLL ALGISTACYG ETYPAPIGPS QSDFGGVGLL QTPTARMARE GELSLNYRDN DQYRYYSASV QLFPWLETTL RYTDVRTRQY SSVEAFSGDQ TYKDKAFDLK LRLWEESYWL PQVAVGARDI GGTGLFDAEY LVASKAWGPF DFTLGLGWGY LGTSGNVKNP LCSASDKYCY RDNSYKQAGS IDGSQMFHGP ASLFGGVEYQ TPWQPLRLKL EYEGNNYQQD FAGKLEQKSK FNVGAIYRVT DWADVNLSYE RGNTFMFGVT LRTNFNDLRP SYNDNARPQY QPQPQDAILQ HSVVANQLTL LKYNAGLADP QIQAKGDTLY VTGEQVKYRD SREGIIRANR IVMNDLPDGI KTIRITENRL NMPQVTTETD VASLKNHLAG EPLGHETTLA QKRVEPVVPQ STEQGWYIDK SRFDFHIDPV LNQSVGGPEN FYMYQLGVMG TADLWLTDHL LTTGSLFANL ANNYDKFNYT NPPQDSHLPR VRTHVREYVQ NDVYVNNLQA NYFQHLGNGF YGQVYGGYLE TMFGGAGAEV LYRPLDSNWA FGLDANYVKQ RDWRSAKDMM KFTDYSVKTG HLTAYWTPSF AQDVLVKASV GQYLAGDKGG TLEIAKRFDS GVVVGGYATI TNVSKEEYGE GDFTKGVYVS VPLDLFSSGP TRSRAAIGWT PLTRDGGQQL GRKFQLYDMT SDRSVNFR
Length	698
Mol. Wt	78.491 kDa
pI	6.5 (calculated)
Extinction coefficient	138,550 - 138,925 (calc based on 45 Y, 13 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-17	UniProt Manual:Signal Peptides	UniProt:P32689
Domain	29..693	PF06082 Bacterial putative lipoprotein (DUF940)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yjbH taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131855	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948527	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013176	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P32689	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11926	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11926	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948527	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001854	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1870	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yjbF
yjbEFGH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4235637..4235677 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4029 (EcoliWiki Page)	NCBI GEO profiles for yjbH
microarray	GenExpDB:b4029 (EcoliWiki Page)	Summary of data for yjbH from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yjbH Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11926	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1870	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4029
EcoGene	EcoGene:EG11926	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001854	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013176	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YJBH	From SHIGELLACYC
E. coli O157	YJBH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF06082 Bacterial putative lipoprotein (DUF940)
EcoCyc	EcoCyc:EG11926	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11926	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001854	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1870	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013176	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

yjbH:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yjbH Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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