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On One Page

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Product(s) |

Expression|

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Standard Name	yajQ
Gene Synonym(s)	ECK0420, b0426, JW5058^[1], JW5058
Product Desc.	nucleotide binding protein^[2]^[3] Activator of phage Phi6 L segment transcription; Phi6 host factor; binds tRNA, ATP and GTP; low abundance protein^[4]
Product Synonyms(s)	predicted nucleotide binding protein^[1], B0426^[2]^[1], YajQ^[2]^[1] , ECK0420, JW5058, b0426
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yajQ^[2]
Regulation/Activity
Quick Links
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Notes

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YajQ indirectly activates phage Phi6 L segment transcription indirectly by binding to the Phi6 core structural protein P1 (Qiao, 2008). The verified N-terminal sequence observed was PSAF, but the conceptual translation is (M)PSF, lacking the alanine residue, possibly explained by experimental error (Saveanu, 2002). NMR solution structure determined for E. coli YajQ (Saveanu, 2002). otherPDB (H. influenzae): 1IN0. Binds TrxA (Kumar, 2004).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yajQ
Mnemonic	Systematic nomenclature
Synonyms	ECK0420, b0426, JW5058^[1], JW5058
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	9.57 minutes	MG1655: 443907..444398 <gbrowseImage> name=NC_000913:443907..444398 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 413105..413596 <gbrowseImage> name=NC_012967:413105..413596 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 346666..347157 <gbrowseImage> name=NC_012759:346666..347157 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 443907..444398 <gbrowseImage> name=NC_007779:443907..444398 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 383238..383729 <gbrowseImage> name=NC_010473:383238..383729 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	443910	Edman degradation	PMID:12381839
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

yajQ(del) (Keio:JW5058)

deletion

PMID:16738554

Shigen

CGSC8295^[5]

yajQ737(del)::kan

PMID:16738554

CGSC:99639

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5058	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCATCTTTCGATATTGTCTC Primer 2:CCATCGCGGAAGTTTTTGAACTG
19F6	Kohara Phage	Genobase	PMID:3038334
2H5	Kohara Phage	Genobase	PMID:3038334
tsx-247::Tn10	Linked marker	CAG12148 = CGSC7337^[5]	est. P1 cotransduction: 65% ^[6]
zaj-3054::Tn10	Linked marker	CAG12017 = CGSC7339^[5]	est. P1 cotransduction: 58% ^[6] Synonyms:zba-3054::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6240	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13613	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002851	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945063	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3379	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001478	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YajQ
Synonyms	predicted nucleotide binding protein^[1], B0426^[2]^[1], YajQ^[2]^[1] , ECK0420, JW5058, b0426
Product description	nucleotide binding protein^[2]^[3] Activator of phage Phi6 L segment transcription; Phi6 host factor; binds tRNA, ATP and GTP; low abundance protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	PMID:12381839	IDA: Inferred from Direct Assay		F		complete
	GO:0000049	tRNA binding	PMID:12381839	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dnaN	PMID:15690043	Experiment(s):EBI-885832
Protein	napD	PMID:15690043	Experiment(s):EBI-892385, EBI-892823
Protein	napD	PMID:19402753	LCMS(ID Probability):99.6
Protein	dnaN	PMID:19402753	MALDI(Z-score):35.939369
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKGEEKMPSF DIVSEVDLQE ARNAVDNASR EVESRFDFRN VEASFELNDA SKTIKVLSES DFQVNQLLDI LRAKLLKRGI EGSSLDVPEN IVHSGKTWFV EAKLKQGIES ATQKKIVKMI KDSKLKVQAQ IQGDEIRVTG KSRDDLQAVM AMVRGGDLGQ PFQFKNFRD
Length	169
Mol. Wt	19.046 kDa
pI	5.7 (calculated)
Extinction coefficient	5,500 (calc based on 0 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..162	PF04461 Protein of unknown function (DUF520)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yajQ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111132	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945063	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001478	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A8E7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6240	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13613	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945063	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002851	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3379	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	3.19E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	128.245+/-0.651	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.27908+/-0.02749	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.49389002	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	14080	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4633	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8949	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yajQ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:443887..443927 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0426 (EcoliWiki Page)	NCBI GEO profiles for yajQ
microarray	GenExpDB:b0426 (EcoliWiki Page)	Summary of data for yajQ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yajQ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6240	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3379	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0426
EcoGene	EcoGene:EG13613	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002851	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001478	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000034691 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YAJQ	From SHIGELLACYC
E. coli O157	YAJQ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04461 Protein of unknown function (DUF520)
Superfamily (EcoliWiki Page)	SUPERFAMILY:89963
Superfamily (EcoliWiki Page)	SUPERFAMILY:89963
EcoCyc	EcoCyc:G6240	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13613	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002851	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3379	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001478	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

yajQ:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yajQ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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