yahE:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

yahE

Gene Synonym(s)

ECK0317, b0319, JW0311[1], JW0311

Product Desc.

predicted protein[2][3]

Function unknown[4]

Product Synonyms(s)

predicted protein[1], B0319[2][1], YahE[2][1] , ECK0317, JW0311, b0319

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yahD[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yahE

Mnemonic

Systematic nomenclature

Synonyms

ECK0317, b0319, JW0311[1], JW0311

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

7.22 minutes 

MG1655: 335149..336012
<gbrowseImage> name=NC_000913:335149..336012 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 308574..309437
<gbrowseImage> name=NC_012967:308574..309437 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 335149..336012
<gbrowseImage> name=NC_007779:335149..336012 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 310699..311562
<gbrowseImage> name=NC_010473:310699..311562 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yahE(del) (Keio:JW0311)

deletion

deletion

PMID:16738554

Shigen

CGSC8511[5]

yahE::Tn5KAN-I-SceI (FB20140)

Insertion at nt 289 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20140

does not contain pKD46

yahE::Tn5KAN-I-SceI (FB20141)

Insertion at nt 289 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20141

contains pKD46

yahE761(del)::kan

PMID:16738554

CGSC:103568


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0311

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTGGGCGCTAACTGCGGATGC

Primer 2:CCACTGACATAATTCCCTCCGTA

6E6

Kohara Phage

Genobase

PMID:3038334

1F6

Kohara Phage

Genobase

PMID:3038334

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[5]

est. P1 cotransduction: 1% [6]

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[5]

est. P1 cotransduction: 29% [6]
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6184

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13589

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002803

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947134

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3359

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001097

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YahE

Synonyms

predicted protein[1], B0319[2][1], YahE[2][1] , ECK0317, JW0311, b0319

Product description

predicted protein[2][3]

Function unknown[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1136211

Protein

nadE

PMID:16606699

Experiment(s):EBI-1136211

Protein

groL

PMID:16606699

Experiment(s):EBI-1136211

Protein

gfcD

PMID:16606699

Experiment(s):EBI-1136211

Protein

arnA

PMID:16606699

Experiment(s):EBI-1136211

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MWALTADADF LAQRGQGQVE QVFARAVNIA LPARQQLLTL LCEEYDNAPN SCRLALTHFD
DLFRHGDKVQ FDDQGITVGQ HLHIEMSRCR RWLSPTLQMT AVNFHLIAWL QWHDIIHQHL
GENETLFNYR GDNPFYQALN KELHIKRRAV IQAVNDKQNI ASAVASMMGL GIGLTPSADD
YLTGLALILF IPGHPAEKYK EEFYLGLQRG KNNTTLLSAI TLEAALQQRC RENIHRFIHN
IIYDIPGNAT QAIEKIKHIG SSSGCDMLYG MADGCALSQT YGGNYVS
Length

287

Mol. Wt

32.266 kDa

pI

6.7 (calculated)

Extinction coefficient

36,900 - 37,650 (calc based on 10 Y, 4 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

167..277

PF11392 Protein of unknown function (DUF2877)

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yahE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128304

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947134

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001097

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77297

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6184

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13589

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947134

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002803

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3359

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yahD

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:335129..335169 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0319 (EcoliWiki Page)

NCBI GEO profiles for yahE

microarray

GenExpDB:b0319 (EcoliWiki Page)

Summary of data for yahE from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to yahE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6184

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3359

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0319

EcoGene

EcoGene:EG13589

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002803

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001097

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

E. coli O157

YAHE

From ECOO157CYC

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

Pfam (EcoliWiki Page)

PF11392 Protein of unknown function (DUF2877)


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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