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Gene |

Product(s) |

Expression|

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Standard Name	uspG
Gene Synonym(s)	ECK0601, b0607, JW0600, ybdQ, up12, yzzU^[1], UP12, yzzU
Product Desc.	universal stress protein UP12^[2]^[3]; Component of universal stress protein UP12^[3] Stress protein, induced in stationary phase; native GroEL substrate; heat shock; mutant sensitive to CCCP; dimeric^[4]
Product Synonyms(s)	universal stress protein UP12^[1], YzzU^[2]^[1], UspG^[2]^[1], YbdQ^[2]^[1], B0607^[2]^[1], UP12^[2]^[1] , ECK0601, JW0600, UP12, ybdQ, yzzU, b0607
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): uspG^[2]
Regulation/Activity
Quick Links
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Notes

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An isoform of UspG is adenylated/phosphorylated; UspG has intrinsic autophosphorylation and autoadenylation activity.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	uspG
Mnemonic	Universal stress protein
Synonyms	ECK0601, b0607, JW0600, ybdQ, up12, yzzU^[1], UP12, yzzU
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	13.81 minutes	MG1655: 641090..640662 <gbrowseImage> name=NC_000913:640662..641090 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 623490..623918 <gbrowseImage> name=NC_012967:623490..623918 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 543422..543850 <gbrowseImage> name=NC_012759:543422..543850 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 641090..640662 <gbrowseImage> name=NC_007779:640662..641090 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 580422..579994 <gbrowseImage> name=NC_010473:579994..580422 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 693682..693254 <gbrowseImage> name=NC_010473:693254..693682 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	640662	Edman degradation	PMID:8740179 PMID:9298646 PMID:9600841
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔuspG (Keio:JW0600)	deletion	deletion	PMID:16738554	Shigen CGSC8715^[5]
ΔuspG746::kan			PMID:16738554	CGSC:103338
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0600	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTATAAGACAATCATTATGCC Primer 2:CCACGCACAACCAGCACCGGCAG
4A5	Kohara Phage	Genobase	PMID:3038334
3G5	Kohara Phage	Genobase	PMID:3038334
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: 85% ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
crcA280::Tn10	Linked marker	CAG12077 = CGSC7347^[5]	est. P1 cotransduction: 62% ^[6] Synonyms:zbe-280::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6334	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12683	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002941	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945229	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2547	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002095	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	UspG
Synonyms	universal stress protein UP12^[1], YzzU^[2]^[1], UspG^[2]^[1], YbdQ^[2]^[1], B0607^[2]^[1], UP12^[2]^[1] , ECK0601, JW0600, UP12, ybdQ, yzzU, b0607
Product description	universal stress protein UP12^[2]^[3]; Component of universal stress protein UP12^[3] Stress protein, induced in stationary phase; native GroEL substrate; heat shock; mutant sensitive to CCCP; dimeric^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	PMID:16460009^[7]	IDA: Inferred from Direct Assay		C		complete
	GO:0046777	protein amino acid autophosphorylation	PMID:16460009^[7]	IDA: Inferred from Direct Assay		P		complete
	GO:0005515	protein binding	PMID:16460009^[7]	IPI: Inferred from Physical Interaction	EcoliWiki:uspG	F	UspG is homodimeric (Figure 8).	complete
	GO:0004017	adenylate kinase activity	PMID:16460009^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0018117	protein amino acid adenylylation	PMID:16460009^[7]	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of universal stress protein UP12	could be indirect
Protein	dnaJ		PMID:15690043^[8]	Experiment(s):EBI-878894
Protein	dnaK		PMID:15690043^[8]	Experiment(s):EBI-878894
Protein	groL		PMID:15690043^[8]	Experiment(s):EBI-878894
Protein	hldD		PMID:15690043^[8]	Experiment(s):EBI-878894
Protein	secA		PMID:15690043^[8]	Experiment(s):EBI-878894
Protein	tufA		PMID:15690043^[8]	Experiment(s):EBI-878894, EBI-894882
Protein	ycaO		PMID:15690043^[8]	Experiment(s):EBI-878894
Protein	putA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	ahpF		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	sdhA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	xylA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	dnaJ		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	groL		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	rpoC		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	glpD		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	dnaK		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	dadA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	atpA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	dnaE		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	lacZ		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	carA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	ppsA		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	mukB		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	rpoB		PMID:16606699^[9]	Experiment(s):EBI-1137384
Protein	rplL		PMID:15690043^[8]	Experiment(s):EBI-882058
Protein	rplW		PMID:15690043^[8]	Experiment(s):EBI-882058
Protein	secB		PMID:15690043^[8]	Experiment(s):EBI-882058
Protein	artJ		PMID:16606699^[9]	Experiment(s):EBI-1138204
Protein	rfaD		PMID:19402753^[10]	LCMS(ID Probability):94.6 MALDI(Z-score):39.255784
Protein	ycaO		PMID:19402753^[10]	MALDI(Z-score):21.221468
Protein	secA		PMID:19402753^[10]	MALDI(Z-score):18.686390
Protein	groS		PMID:19402753^[10]	MALDI(Z-score):28.178722
Protein	dnaJ		PMID:19402753^[10]	MALDI(Z-score):36.982560
Protein	rplL		PMID:19402753^[10]	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Cytoplasm		PMID:8740179, PMID:9298646, PMID:9600841, PMID:12071968, PMID:16460009	EchoLocation:uspG
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MYKTIIMPVD VFEMELSDKA VRHAEFLAQD DGVIHLLHVL PGSASLSLHR FAADVRRFEE HLQHEAQERL QTMVSHFTID PSRIKQHVRF GSVRDEVNEL AEELGADVVV IGSRNPSIST HLLGSNASSV IRHANLPVLV VR
Length	142
Mol. Wt	15.934 kDa
pI	6.5 (calculated)
Extinction coefficient	1,490 (calc based on 1 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Modification Site	128	phosphorylation site at S128	probability greater than 75%	PMID:17938405^[11]
Domain	1..142	PF00582 Universal stress protein family		PMID:19920124^[12]
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<motif_map/>

Structure
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Structures

<beststructure> gene=uspG taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128590	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945229	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002095	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39177	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6334	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12683	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945229	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002941	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2547	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	6.13E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	80.329+/-0.578	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	980	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	789	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	637	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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Transcription unit(s)
uspG
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:641070..641110 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

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Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0607 (EcoliWiki Page)	NCBI GEO profiles for uspG
microarray	GenExpDB:b0607 (EcoliWiki Page)	Summary of data for uspG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (641013..641371) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ14; Well:F9^[13]
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Notes

Accessions Related to uspG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6334	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2547	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0607
EcoGene	EcoGene:EG12683	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002941	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002095	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YBDQ	From SHIGELLACYC
E. coli O157	YBDQ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:52402
Pfam (EcoliWiki Page)	PF00582 Universal stress protein family
EcoCyc	EcoCyc:G6334	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12683	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002941	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2547	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002095	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^7.0 ^7.1 ^7.2 ^7.3 ^7.4 Weber, A & Jung, K (2006) Biochemical properties of UspG, a universal stress protein of Escherichia coli. Biochemistry 45 1620-8 PubMed
↑ ^8.00 ^8.01 ^8.02 ^8.03 ^8.04 ^8.05 ^8.06 ^8.07 ^8.08 ^8.09 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
↑ ^9.00 ^9.01 ^9.02 ^9.03 ^9.04 ^9.05 ^9.06 ^9.07 ^9.08 ^9.09 ^9.10 ^9.11 ^9.12 ^9.13 ^9.14 ^9.15 ^9.16 ^9.17 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ ^10.0 ^10.1 ^10.2 ^10.3 ^10.4 ^10.5 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
↑ Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

uspG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to uspG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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