uspF:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uspF

Mnemonic

Universal stress protein

Synonyms

ECK1371, b1376, JW1370, ynaF, yzzL, up03[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

30.89 minutes 

MG1655: 1433643..1433209
<gbrowseImage> name=NC_000913:1433209..1433643 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1325268..1325702
<gbrowseImage> name=NC_012759:1325268..1325702 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1437333..1436899
<gbrowseImage> name=NC_007779:1436899..1437333 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1524238..1523804
<gbrowseImage> name=NC_010473:1523804..1524238 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1433209

Edman degradation

PMID:8740179[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔuspF (Keio:JW1370)

deletion

deletion

PMID:16738554[4]

Shigen

ΔyzzL::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938[5]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1370

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAACAGAACGATTCTTGTCCC

Primer 2:CCGCGCACAACCAGCACGGAGCA

4E7

Kohara Phage

Genobase

PMID:3038334[7]

1E7

Kohara Phage

Genobase

PMID:3038334[7]

recT3061::Tn10

Linked marker

CAG12081 = CGSC7375[8]

est. P1 cotransduction: 43% [9]
Synonyms:zcj-3061::Tn10, zda-3061::Tn10

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[8]

est. P1 cotransduction: 99% [9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6699

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12674

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003294

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945948

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2539

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004605

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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