tufA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
tufA |
|---|---|
| Mnemonic |
Translation unstable factor |
| Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
74.75 minutes |
MG1655: 3469351..3468167 |
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NC_012967: 3399234..3398050 |
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NC_012759: 3355324..3356508 |
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W3110 |
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W3110: 4169087..4170271 |
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DH10B: 3567096..3565912 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3468170 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔtufA (Keio:JW3301) |
deletion |
deletion |
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tufAE349A |
E349A |
No change in binding GDP but higher binding constant for EF-Ts |
seeded from UniProt:P0A6N1 | ||||
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tufAA376T,V |
A376T,V |
Kirromycin resistant |
seeded from UniProt:P0A6N1 | ||||
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tufAQ115A |
Q115A |
Weaker binding for GDP and for EF- Ts |
seeded from UniProt:P0A6N1 | ||||
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tufAQ125K |
Q125K |
Kirromycin resistant |
seeded from UniProt:P0A6N1 | ||||
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tufAK137R,Q,E,I |
K137R,Q,E,I |
Reduces affinity for GDP |
seeded from UniProt:P0A6N1 | ||||
|
tufAD139N |
D139N |
Reduces affinity for GDP; increases affinity for XDP |
seeded from UniProt:P0A6N1 | ||||
|
tufAG223D |
G223D |
Inhibits codon-induced conformational changes leading to GTPase activation on the ribosome |
seeded from UniProt:P0A6N1 | ||||
|
tufAR231C |
R231C |
Pulvomycin resistant |
seeded from UniProt:P0A6N1 | ||||
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tufAG317D |
G317D |
Kirromycin resistant |
seeded from UniProt:P0A6N1 | ||||
|
tufAH20A |
H20A |
No change in binding GDP and 3-fold reduction in binding EF-Ts |
seeded from UniProt:P0A6N1 | ||||
|
tufAV21G |
V21G |
Lowers GTPase activity 5 to 10- fold |
seeded from UniProt:P0A6N1 | ||||
|
tufAP83T |
P83T |
Loss of GTPase activity and creation of an autophosphorylation site |
seeded from UniProt:P0A6N1 | ||||
|
tufAR334C |
R334C |
Pulvomycin resistant |
seeded from UniProt:P0A6N1 | ||||
|
tufAT335A |
T335A |
Pulvomycin resistant |
seeded from UniProt:P0A6N1 | ||||
|
tufAS394G |
S394G |
(in tufA) |
Strain variation; seeded from UniProt:P0A6N1 | ||||
|
tufA1 |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3301 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCTAAAGAAAAATTTGAACG Primer 2:CCGCCCAGAACTTTAGCAACAAC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 19% [10] | ||
|
Linked marker |
est. P1 cotransduction: 24% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Jones, MD et al. (1980) The complete amino-acid sequence of elongation factor Tu from Escherichia coli. Eur. J. Biochem. 108 507-26 PubMed
- ↑ Laursen, RA et al. (1981) The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence. J. Biol. Chem. 256 8102-9 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
