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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	trpE
Gene Synonym(s)	ECK1258, b1264, JW1256, anth, tryD, tryp-4, tryp-^[1]^[2]
Product Desc.	anthranilate synthase component I^[2]^[3]; Component of anthranilate synthase^[2]^[3] Anthranilate synthase component I^[4]
Product Synonyms(s)	component I of anthranilate synthase^[1], B1264^[2]^[1], TryD^[2]^[1], TrpE^[2]^[1], α subunit^[2]^[1] , anth, ECK1258, JW1256, tryD, tryp-4, b1264
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): trpLEDCBA^[2], OP00094, trp
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Induced during biofilm formation.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	trpE
Mnemonic	Tryptophan
Synonyms	ECK1258, b1264, JW1256, anth, tryD, tryp-4, tryp-^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	28.44 minutes	MG1655: 1320970..1319408 <gbrowseImage> name=NC_000913:1319408..1320970 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1320540..1318978 <gbrowseImage> name=NC_012967:1318978..1320540 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1210257..1211819 <gbrowseImage> name=NC_012759:1210257..1211819 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1324660..1323098 <gbrowseImage> name=NC_007779:1323098..1324660 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1410366..1408804 <gbrowseImage> name=NC_010473:1408804..1410366 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1319408	Edman degradation	PMID:4598537
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
trpE(del) (Keio:JW1256)	deletion	deletion			PMID:16738554	Shigen CGSC9133^[5]
trpEC13S		C13S		(in strain: TRPA2/COLVB)			Strain variation; seeded from UniProt:P00895
trpE81						CGSC:4847
trpE38						CGSC:4955
trpE3(Oc)			Auxotrophies	requires anthranilate, indole, or tryptophan for growth	Smith and Yanofsky (1962)^[6]	CGSC:5086	ochre (UAA) mutation
trpE9851(Oc)						CGSC:5337	ochre (UAA) mutation
trpE61						CGSC:5690
trpE68						CGSC:7045
trpE65(Oc)						CGSC:8489	ochre (UAA) mutation
trpE9829(Am)					PMID:326769 PMID:4945859	CGSC:8787	amber (UAG) mutation
trpE63(del)						CGSC:9689
trpE10220					PMID:5339605	CGSC:65105
trpE719(fbR)				feedback resistant	PMID:4869215	CGSC:67294
trpE9997						CGSC:80584
trpE772(del)::kan					PMID:16738554	CGSC:103230
treP(del)	deletion	deletion	Motility	Deletion mutation increase motility compared to wild-type strain, figure 6.	PMID:17511876
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1256	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAAACACAAAAACCGACTCT Primer 2:CCGAAAGTCTCCTGTGCATGATG
4F1	Kohara Phage	Genobase	PMID:3038334
13F9	Kohara Phage	Genobase	PMID:3038334
trpC83::Tn10	Linked marker	CAG18455 = CGSC7371^[5]	est. P1 cotransduction: 94% ^[7] Synonyms:trpB83::Tn10
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 10% ^[7] Synonyms:zci-233::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11028	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11028	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001017	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945846	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1021	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004244	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TrpE
Synonyms	component I of anthranilate synthase^[1], B1264^[2]^[1], TryD^[2]^[1], TrpE^[2]^[1], α subunit^[2]^[1] , anth, ECK1258, JW1256, tryD, tryp-4, b1264
Product description	anthranilate synthase component I^[2]^[3]; Component of anthranilate synthase^[2]^[3] Anthranilate synthase component I^[4]
EC number (for enzymes)	4.1.3.27^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000162	tryptophan biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0822	P	Seeded from EcoCyc (v14.0)	complete
	GO:0004049	anthranilate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005257	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004049	anthranilate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.3.27	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004049	anthranilate synthase activity	PMID:4886289	IDA: Inferred from Direct Assay		F	Table 1	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	TrpD	PMID:4886290	zone centrifugation
Protein	acnB	PMID:16606699	Experiment(s):EBI-1139361
Protein	ompL	PMID:16606699	Experiment(s):EBI-1139361
Protein	insI1	PMID:16606699	Experiment(s):EBI-1139361, EBI-1139361, EBI-1139361
Protein	fusA	PMID:16606699	Experiment(s):EBI-1139361
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1139361
Protein	htpG	PMID:16606699	Experiment(s):EBI-1139361
Protein	lacZ	PMID:16606699	Experiment(s):EBI-1139361
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1139361
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1139361
Protein	mukB	PMID:16606699	Experiment(s):EBI-1139361
Protein	rpsA	PMID:16606699	Experiment(s):EBI-1139361
Protein	rplD	PMID:16606699	Experiment(s):EBI-1139361
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1139361
Protein	nadE	PMID:16606699	Experiment(s):EBI-1139361
Protein	groL	PMID:16606699	Experiment(s):EBI-1139361
Protein	rplF	PMID:16606699	Experiment(s):EBI-1139361
Protein	aceE	PMID:16606699	Experiment(s):EBI-1139361
Protein	rplB	PMID:16606699	Experiment(s):EBI-1139361
Protein	nuoE	PMID:16606699	Experiment(s):EBI-1139361
Protein	Subunits of anthranilate synthase
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQTQKPTLEL LTCEGAYRDN PTALFHQLCG DRPATLLLES ADIDSKDDLK SLLLVDSALR ITALGDTVTI QALSGNGEAL LALLDNALPA GVESEQSPNC RVLRFPPVSP LLDEDARLCS LSVFDAFRLL QNLLNVPKEE REAMFFGGLF SYDLVAGFED LPQLSAENNC PDFCFYLAET LMVIDHQKKS TRIQASLFAP NEEEKQRLTA RLNELRQQLT EAAPPLPVVS VPHMRCECNQ SDEEFGGVVR LLQKAIRAGE IFQVVPSRRF SLPCPSPLAA YYVLKKSNPS PYMFFMQDND FTLFGASPES SLKYDATSRQ IEIYPIAGTR PRGRRADGSL DRDLDSRIEL EMRTDHKELS EHLMLVDLAR NDLARICTPG SRYVADLTKV DRYSYVMHLV SRVVGELRHD LDALHAYRAC MNMGTLSGAP KVRAMQLIAE AEGRRRGSYG GAVGYFTAHG DLDTCIVIRS ALVENGIATV QAGAGVVLDS VPQSEADETR NKARAVLRAI ATAHHAQETF
Length	520
Mol. Wt	57.495 kDa
pI	5.3 (calculated)
Extinction coefficient	20,860 - 22,360 (calc based on 14 Y, 0 W, and 12 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	17..193	PF04715 Anthranilate synthase component I, N terminal region	PMID:19920124
Domain	241..502	PF00425 chorismate binding enzyme	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=trpE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129225	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945846	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004244	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00895	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11028	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11028	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945846	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001017	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1021	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	121	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1153	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	319	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
trpLEDCBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1320950..1320990 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1264 (EcoliWiki Page)	NCBI GEO profiles for trpE
microarray	GenExpDB:b1264 (EcoliWiki Page)	Summary of data for trpE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to trpE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11028	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1021	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1264
EcoGene	EcoGene:EG11028	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001017	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004244	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Saccharomyces cerevisiae	YER090W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC14429 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000033298 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	TRPE	From SHIGELLACYC
E. coli O157	TRPE_1	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04715 Anthranilate synthase component I, N terminal region
Pfam (EcoliWiki Page)	PF00425 chorismate binding enzyme
Panther (EcoliWiki Page)	PTHR11236:SF9
Superfamily (EcoliWiki Page)	SUPERFAMILY:56322
EcoCyc	EcoCyc:EG11028	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11028	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001017	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1021	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004244	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Smith, O.H. and C. Yanofsky (1962) Enzymes involved in the Biosynthesis of Tryptophan. Methods Enzymol. 5:794-806.
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

trpE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to trpE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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