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Standard Name	tolQ
Gene Synonym(s)	ECK0726, b0737, JW0727, fii, tolP^[1], tolP
Product Desc.	TolQ^[2]^[3]; Component of Tol-Pal Cell Envelope Complex^[2]; The Tol-Pal Cell Envelope Complex^[3]; The Colicin A Import System^[3]; Colicin S4 Transport System^[3] Tolerance to group A colicins and ssDNA filamentous phage; cell envelope integrity; inner membrane protein^[4]
Product Synonyms(s)	membrane spanning protein in TolA-TolQ-TolR complex^[1], B0737^[2]^[1], Fii^[2]^[1], TolP^[2]^[1], TolQ^[2]^[1] , ECK0726, fii, JW0727, tolP, b0737
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ybgC-tolQRA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tolQ
Mnemonic	Tolerance
Synonyms	ECK0726, b0737, JW0727, fii, tolP^[1], tolP
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	16.69 minutes	MG1655: 774376..775068 <gbrowseImage> name=NC_000913:774376..775068 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 756035..756727 <gbrowseImage> name=NC_012967:756035..756727 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 677136..677828 <gbrowseImage> name=NC_012759:677136..677828 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 775575..776267 <gbrowseImage> name=NC_007779:775575..776267 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 826968..827660 <gbrowseImage> name=NC_010473:826968..827660 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	774376	Edman degradation	PMID:8300535
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
tolQ(del) (Keio:JW0727)	deletion	deletion			PMID:16738554	Shigen CGSC8793^[5]
tolQ7	ATCCAG -> ATTTAG^[6]	Ile-Gln to Ile-UAG stop codon	Resistant to	resistant to phage f1	PMID:3001021	CGSC:92683	fii-1 in TPS13 Table 2
tolQ9					PMID:3001021	CGSC:92695	'fii-6 in TPS94
tolQ786(del)::kan					PMID:16738554	CGSC:103168
tolQ(fii-5)	GGT -> GAT ^[6]	Gly codon to Asp codon	Resistant to	resistant to phage f1	PMID:3001021	CGSC:92683	fii-5 in TPS66 Table 2
tolQ(fii-5)	GGT -> GAT ^[6]	Gly codon to Asp codon	Resistant to	resistant to colicins E1 and E2	PMID:3001021	CGSC:92683	fii-5 in TPS66 Table 3
tolQ7	ATCCAG -> ATTTAG^[6]	Ile-Gln to Ile-UAG stop codon	Resistant to	resistant to colicins E1 and E2	PMID:3001021	CGSC:92683	fii-1 in TPS13 Table 3
tolQ(fii-5)	GGT -> GAT ^[6]	Gly codon to Asp codon	Resistant to	still able to plaque phage P1	PMID:3001021	CGSC:92683	fii-5 in TPS66 Table 2
tolQ7	ATCCAG -> ATTTAG^[6]	Ile-Gln to Ile-UAG stop codon	Resistant to	doesn't plaque phage P1, but can act as recipient for P1 transduction	PMID:3001021	CGSC:92683	fii-1 in TPS13 Table 2
tolQ overexpression				null-FtsN-like division phenotype	PMID:23818486
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0727	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACTGACATGAATATCCTTGA Primer 2:CCCCCCTTGTTGCTCTCGCTAAC
18F11	Kohara Phage	Genobase	PMID:3038334
10G5	Kohara Phage	Genobase	PMID:3038334
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[5]	est. P1 cotransduction: 1% ^[7] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
nadA57::Tn10	Linked marker	CAG12147 = CGSC7351^[5]	est. P1 cotransduction: 84% ^[7]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11010	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11010	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000999	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948900	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1003	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002511	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TolQ
Synonyms	membrane spanning protein in TolA-TolQ-TolR complex^[1], B0737^[2]^[1], Fii^[2]^[1], TolP^[2]^[1], TolQ^[2]^[1] , ECK0726, fii, JW0727, tolP, b0737
Product description	TolQ^[2]^[3]; Component of Tol-Pal Cell Envelope Complex^[2]; The Tol-Pal Cell Envelope Complex^[3]; The Colicin A Import System^[3]; Colicin S4 Transport System^[3] Tolerance to group A colicins and ssDNA filamentous phage; cell envelope integrity; inner membrane protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:10419942	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:7744737	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	PMID:8331075	IDA: Inferred from Direct Assay		C		complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	PMID:8331075	IDA: Inferred from Direct Assay		C		complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0017038	protein import	PMID:2651401	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002898	P	Seeded from EcoCyc (v14.0)	complete
	GO:0043213	bacteriocin transport	PMID:2651401	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:7744737	IDA: Inferred from Direct Assay		F	with TolA	complete
	GO:0008565	protein transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002898	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:10419942	IDA: Inferred from Direct Assay		F	with TolR	complete
	GO:0015031	protein transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0653	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0043213	bacteriocin transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0080	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of Tol-Pal Cell Envelope Complex	could be indirect
Protein	Subunits of The Tol-Pal Cell Envelope Complex	could be indirect
Protein	Subunits of The Colicin A Import System	could be indirect
Protein	Subunits of Colicin S4 Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 3 predicted transmembrane domains	Daley et al. (2005) ^[8]
Inner Membrane		PMID:8300535, PMID:8331075	EchoLocation:tolQ
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTDMNILDLF LKASLLVKLI MLILIGFSIA SWAIIIQRTR ILNAAAREAE AFEDKFWSGI ELSRLYQESQ GKRDNLTGSE QIFYSGFKEF VRLHRANSHA PEAVVEGASR AMRISMNREL ENLETHIPFL GTVGSISPYI GLFGTVWGIM HAFIALGAVK QATLQMVAPG IAEALIATAI GLFAAIPAVM AYNRLNQRVN KLELNYDNFM EEFTAILHRQ AFTVSESNKG
Length	230
Mol. Wt	25.598 kDa
pI	7.1 (calculated)
Extinction coefficient	23,950 (calc based on 5 Y, 3 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	73..211	PF01618 MotA/TolQ/ExbB proton channel family		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tolQ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128712	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948900	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002511	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABU9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11010	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11010	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948900	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000999	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1003	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	4.05E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1667	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	392	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	639	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ybgC-tolQRA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:774356..774396 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0737 (EcoliWiki Page)	NCBI GEO profiles for tolQ
microarray	GenExpDB:b0737 (EcoliWiki Page)	Summary of data for tolQ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to tolQ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11010	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1003	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0737
EcoGene	EcoGene:EG11010	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000999	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002511	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000022132 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	TOLQ	From SHIGELLACYC
E. coli O157	TOLQ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01618 MotA/TolQ/ExbB proton channel family
EcoCyc	EcoCyc:EG11010	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11010	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000999	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1003	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002511	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 Sun, TP & Webster, RE (1987) Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli. J. Bacteriol. 169 2667-74 PubMed
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

tolQ:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tolQ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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