tolQ:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
tolQ |
|---|---|
| Mnemonic |
Tolerance |
| Synonyms |
ECK0726, b0737, JW0727, fii, tolP[1], tolP |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
16.69 minutes |
MG1655: 774376..775068 |
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NC_012967: 756035..756727 |
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NC_012759: 677136..677828 |
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W3110 |
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W3110: 775575..776267 |
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DH10B: 826968..827660 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
774376 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
tolQ(del) (Keio:JW0727) |
deletion |
deletion |
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tolQ7 |
ATCCAG -> ATTTAG[5] |
Ile-Gln to Ile-UAG stop codon |
Resistant to |
resistant to phage f1 |
fii-1 in TPS13 Table 2 | ||
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tolQ9 |
'fii-6 in TPS94 | ||||||
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tolQ786(del)::kan |
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tolQ(fii-5) |
GGT -> GAT [5] |
Gly codon to Asp codon |
Resistant to |
resistant to phage f1 |
fii-5 in TPS66 Table 2 | ||
|
tolQ(fii-5) |
GGT -> GAT [5] |
Gly codon to Asp codon |
Resistant to |
resistant to colicins E1 and E2 |
fii-5 in TPS66 Table 3 | ||
|
tolQ7 |
ATCCAG -> ATTTAG[5] |
Ile-Gln to Ile-UAG stop codon |
Resistant to |
resistant to colicins E1 and E2 |
fii-1 in TPS13 Table 3 | ||
|
tolQ(fii-5) |
GGT -> GAT [5] |
Gly codon to Asp codon |
Resistant to |
still able to plaque phage P1 |
fii-5 in TPS66 Table 2 | ||
|
tolQ7 |
ATCCAG -> ATTTAG[5] |
Ile-Gln to Ile-UAG stop codon |
Resistant to |
doesn't plaque phage P1, but can act as recipient for P1 transduction |
fii-1 in TPS13 Table 2 | ||
|
tolQ overexpression |
null-FtsN-like division phenotype |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0727 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACTGACATGAATATCCTTGA Primer 2:CCCCCCTTGTTGCTCTCGCTAAC | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 1% [10] | ||
|
Linked marker |
est. P1 cotransduction: 84% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Vianney, A et al. (1994) Membrane topology and mutational analysis of the TolQ protein of Escherichia coli required for the uptake of macromolecules and cell envelope integrity. J. Bacteriol. 176 822-9 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Sun, TP & Webster, RE (1987) Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli. J. Bacteriol. 169 2667-74 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 Sun, TP & Webster, RE (1986) fii, a bacterial locus required for filamentous phage infection and its relation to colicin-tolerant tolA and tolB. J. Bacteriol. 165 107-15 PubMed
- ↑ Teleha, MA et al. (2013) Overexpression of the Escherichia coli TolQ protein leads to a null-FtsN-like division phenotype. Microbiologyopen 2 618-32 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
