{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	tesA
Gene Synonym(s)	ECK0488, b0494, JW0483, apeA, pldC^[1], pldC
Product Desc.	Acyl-CoA thioesterase I; also protease I; also lysophospholipase L1; monomeric^[2]
Product Synonyms(s)	multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1^[1], B0494^[3]^[1], PldC^[3]^[1], ApeA^[3]^[1], TesA^[3]^[1], long chain fatty acyl thioesterase I^[3]^[1], lysophospholipase L₁^[3]^[1], lecithinase B^[3]^[1], phospholipase B^[3]^[1], thioesterase I^[3]^[1], acyl-CoA thioesterase I^[3]^[1] , apeA, ECK0488, JW0483, pldC, b0494
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ybbO^[3], tesA^[3]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tesA
Mnemonic	Thioesterase
Synonyms	ECK0488, b0494, JW0483, apeA, pldC^[1], pldC
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	11.17 minutes	MG1655: 518989..518363 <gbrowseImage> name=NC_000913:518363..518989 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 491933..491307 <gbrowseImage> name=NC_012967:491307..491933 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 421122..421748 <gbrowseImage> name=NC_012759:421122..421748 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 518989..518363 <gbrowseImage> name=NC_007779:518363..518989 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 458320..457694 <gbrowseImage> name=NC_010473:457694..458320 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	518366	Edman degradation	PMID:1864840 PMID:8098033 PMID:8432696
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔtesA (Keio:JW0483)	deletion	deletion		PMID:16738554	Shigen CGSC8632^[4]
tesA::Tn5KAN-2 (FB20187)	Insertion at nt 557 in Minus orientation			PMID:15262929	E. coli Genome Project:FB20187	does not contain pKD46
tesAD180A		D180A	Reduces activity by over 70%			seeded from UniProt:P0ADA1
tesAH183A		H183A	Reduces activity by over 99%			seeded from UniProt:P0ADA1
tesAG70A		G70A	Reduces activity by over 75%			seeded from UniProt:P0ADA1
tesAS36A		S36A	Reduces activity by over 99%			seeded from UniProt:P0ADA1
tesAN99A		N99A	Reduces activity by over 60%			seeded from UniProt:P0ADA1
tesAL135P		L135P	Lowers activity towards substrates with long acyl chains			seeded from UniProt:P0ADA1
ΔtesA777::kan				PMID:16738554	CGSC:103126
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0483	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGAACTTCAACAATGTTTT Primer 2:CCTGAGTCATGATTTACTAAAGG
7C12	Kohara Phage	Genobase	PMID:3038334
8E4	Kohara Phage	Genobase	PMID:3038334
gsk-3055::Tn10	Linked marker	CAG12154 = CGSC7341^[4]	est. P1 cotransduction: 54% ^[5] Synonyms:zbb-30-55::TnlO, zba-3055::Tn10
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[4]	est. P1 cotransduction: 26% ^[5] Synonyms:purE79::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11542	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11542	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001496	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945127	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1504	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001713	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TesA
Synonyms	multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1^[1], B0494^[3]^[1], PldC^[3]^[1], ApeA^[3]^[1], TesA^[3]^[1], long chain fatty acyl thioesterase I^[3]^[1], lysophospholipase L₁^[3]^[1], lecithinase B^[3]^[1], phospholipase B^[3]^[1], thioesterase I^[3]^[1], acyl-CoA thioesterase I^[3]^[1] , apeA, ECK0488, JW0483, pldC, b0494
Product description	Acyl-CoA thioesterase I; also protease I; also lysophospholipase L1; monomeric^[2]
EC number (for enzymes)	3.1.2.-^[1] 3.1.1.5^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004622	lysophospholipase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.1.5	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	PMID:8432696			P	check annotation--it may be a peptidase	Missing: evidence
	GO:0006629	lipid metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001087	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006629	lipid metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008265	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008233	peptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0645	F	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[6]
Periplasm	PMID:8098033	EchoLocation:tesA
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMNFNNVFRW HLPFLFLVLL TFRAAAADTL LILGDSLSAG YRMSASAAWP ALLNDKWQSK TSVVNASISG DTSQQGLARL PALLKQHQPR WVLVELGGND GLRGFQPQQT EQTLRQILQD VKAANAEPLL MQIRLPANYG RRYNEAFSAI YPKLAKEFDV PLLPFFMEEV YLKPQWMQDD GIHPNRDAQP FIADWMAKQL QPLVNHDS
Length	208
Mol. Wt	23.622 kDa
pI	7.6 (calculated)
Extinction coefficient	40,450 (calc based on 5 Y, 6 W, and 0 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-26	UniProt Manual:Signal Peptides	UniProt:P0ADA1
Domain	30..196	PF00657 GDSL-like Lipase/Acylhydrolase		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tesA taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128478	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945127	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001713	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ADA1	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11542	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11542	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945127	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001496	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1504	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.86E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	400	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	289	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	247	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ybbO
tesA
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:518969..519009 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0494 (EcoliWiki Page)	NCBI GEO profiles for tesA
microarray	GenExpDB:b0494 (EcoliWiki Page)	Summary of data for tesA from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to tesA Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11542	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1504	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0494
EcoGene	EcoGene:EG11542	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001496	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001713	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000016728 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	TESA	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00657 GDSL-like Lipase/Acylhydrolase
Superfamily (EcoliWiki Page)	SUPERFAMILY:52266
EcoCyc	EcoCyc:EG11542	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11542	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001496	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1504	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001713	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 ^1.20 ^1.21 ^1.22 ^1.23 ^1.24 ^1.25 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 ^3.12 ^3.13 ^3.14 ^3.15 ^3.16 ^3.17 ^3.18 ^3.19 ^3.20 ^3.21 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

tesA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tesA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search