tesA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
tesA |
|---|---|
| Mnemonic |
Thioesterase |
| Synonyms |
ECK0488, b0494, JW0483, apeA, pldC[1], pldC |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
11.17 minutes |
MG1655: 518989..518363 |
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NC_012967: 491933..491307 |
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NC_012759: 421122..421748 |
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W3110 |
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W3110: 518989..518363 |
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DH10B: 458320..457694 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
518366 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔtesA (Keio:JW0483) |
deletion |
deletion |
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tesA::Tn5KAN-2 (FB20187) |
Insertion at nt 557 in Minus orientation |
does not contain pKD46 | |||||
|
tesAD180A |
D180A |
Reduces activity by over 70% |
seeded from UniProt:P0ADA1 | ||||
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tesAH183A |
H183A |
Reduces activity by over 99% |
seeded from UniProt:P0ADA1 | ||||
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tesAG70A |
G70A |
Reduces activity by over 75% |
seeded from UniProt:P0ADA1 | ||||
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tesAS36A |
S36A |
Reduces activity by over 99% |
seeded from UniProt:P0ADA1 | ||||
|
tesAN99A |
N99A |
Reduces activity by over 60% |
seeded from UniProt:P0ADA1 | ||||
|
tesAL135P |
L135P |
Lowers activity towards substrates with long acyl chains |
seeded from UniProt:P0ADA1 | ||||
|
ΔtesA777::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0483 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATGAACTTCAACAATGTTTT Primer 2:CCTGAGTCATGATTTACTAAAGG | |
|
Kohara Phage |
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|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 54% [10] | ||
|
Linked marker |
est. P1 cotransduction: 26% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Karasawa, K et al. (1991) Lysophospholipase L1 from Escherichia coli K-12 overproducer. J. Biochem. 109 288-93 PubMed
- ↑ Cho, H & Cronan, JE Jr (1993) Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme. J. Biol. Chem. 268 9238-45 PubMed
- ↑ Ichihara, S et al. (1993) Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants. J. Bacteriol. 175 1032-7 PubMed
- ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
