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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	sspA
Gene Synonym(s)	ECK3218, b3229, JW3198, pog, ssp^[1], ssp
Product Desc.	stringent starvation protein A^[2]^[3] Activator of phage P1 late genes, stress response protein; glutathione S-transferase homolog^[4]
Product Synonyms(s)	stringent starvation protein A^[1], B3229^[2]^[1], Pog^[2]^[1], Ssp^[2]^[1], SspA^[2]^[1] , ECK3218, JW3198, pog, ssp, b3229
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): sspAB^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	sspA
Mnemonic	Stringent starvation protein
Synonyms	ECK3218, b3229, JW3198, pog, ssp^[1], ssp
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	72.74 minutes	MG1655: 3375442..3374804 <gbrowseImage> name=NC_000913:3374804..3375442 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3305318..3304680 <gbrowseImage> name=NC_012967:3304680..3305318 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3261952..3262590 <gbrowseImage> name=NC_012759:3261952..3262590 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3377275..3376637 <gbrowseImage> name=NC_007779:3376637..3377275 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3473187..3472549 <gbrowseImage> name=NC_010473:3472549..3473187 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3374807	Edman degradation	PMID:3003520 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
sspA(del) (Keio:JW3198)	deletion	deletion			PMID:16738554	Shigen CGSC10427^[5]
sspA::Tn5KAN-I-SceI (FB21076)	Insertion at nt 482 in Minus orientation				PMID:15262929	E. coli Genome Project:FB21076	contains pKD46
sspA757(del)::kan					PMID:16738554	CGSC:103028
sspA(del)::Nm^r	Deletion of sspA gene		Resistant to	resistance to Neomycin developed.	PMID:1721886		Was analyzed by southern-blot, see figure 4 for full analyze.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3198	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCTGTCGCTGCCAACAAACG Primer 2:CCACTCCGaCCCAGACGCATTTCACGTTCT
7C8	Kohara Phage	Genobase	PMID:3038334
1E3	Kohara Phage	Genobase	PMID:3038334
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[5]	est. P1 cotransduction: 18% ^[6] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10977	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10977	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000966	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944744	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0970	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010594	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SspA
Synonyms	stringent starvation protein A^[1], B3229^[2]^[1], Pog^[2]^[1], Ssp^[2]^[1], SspA^[2]^[1] , ECK3218, JW3198, pog, ssp, b3229
Product description	stringent starvation protein A^[2]^[3] Activator of phage P1 late genes, stress response protein; glutathione S-transferase homolog^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006950	response to stress	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpoB	PMID:15690043	Experiment(s):EBI-884735
Protein	rpoC	PMID:15690043	Experiment(s):EBI-884735
Protein	dnaK	PMID:15690043	Experiment(s):EBI-891549
Protein	hrsA	PMID:15690043	Experiment(s):EBI-891549
Protein	lysU	PMID:15690043	Experiment(s):EBI-891549
Protein	groL	PMID:15690043	Experiment(s):EBI-891549
Protein	pflB	PMID:15690043	Experiment(s):EBI-891549
Protein	rplI	PMID:15690043	Experiment(s):EBI-891549
Protein	rpoA	PMID:15690043	Experiment(s):EBI-891549
Protein	serS	PMID:15690043	Experiment(s):EBI-891549
Protein	thiG	PMID:15690043	Experiment(s):EBI-891549
Protein	tyrP	PMID:15690043	Experiment(s):EBI-891549
Protein	ybaT	PMID:15690043	Experiment(s):EBI-891549
Protein	groL	PMID:19402753	LCMS(ID Probability):99.6
Protein	serS	PMID:19402753	LCMS(ID Probability):99.0
Protein	thiG	PMID:19402753	LCMS(ID Probability):99.0
Protein	ybaT	PMID:19402753	LCMS(ID Probability):99.0
Protein	mngA	PMID:19402753	LCMS(ID Probability):99.0
Protein	lysU	PMID:19402753	LCMS(ID Probability):99.0
Protein	tyrP	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAVAANKRSV MTLFSGPTDI YSHQVRIVLA EKGVSFEIEH VEKDNPPQDL IDLNPNQSVP TLVDRELTLW ESRIIMEYLD ERFPHPPLMP VYPVARGESR LYMHRIEKDW YTLMNTIING SASEADAARK QLREELLAIA PVFGQKPYFL SDEFSLVDCY LAPLLWRLPQ LGIEFSGPGA KELKGYMTRV FERDSFLASL TEAEREMRLG RS
Length	212
Mol. Wt	24.305 kDa
pI	5.1 (calculated)
Extinction coefficient	28,420 - 28,545 (calc based on 8 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0ACA3
Domain	101..194	PF00043 Glutathione S-transferase, C-terminal domain		PMID:19920124
Domain	9..81	PF02798 Glutathione S-transferase, N-terminal domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=sspA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131119	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944744	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010594	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACA3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10977	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10977	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944744	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000966	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0970	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.96E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	240.998+/-1.244	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.60774+/-0.08443	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.304858934	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 EMG2	480	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: M119	PMID: 9298646
Protein	E. coli K-12 MG1655	12135	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2668	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4611	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
sspAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3375422..3375462 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3229 (EcoliWiki Page)	NCBI GEO profiles for sspA
microarray	GenExpDB:b3229 (EcoliWiki Page)	Summary of data for sspA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3374976..3375461) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ05; Well:G1^[7]
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Notes

Accessions Related to sspA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10977	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0970	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3229
EcoGene	EcoGene:EG10977	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000966	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010594	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000009071 (score: 1.000; bootstrap: 53%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G78380 (score: 1.000; bootstrap: 79%) AT1G17180 (score: 0.734) AT1G78370 (score: 0.707) AT1G78360 (score: 0.675) AT1G78340 (score: 0.667) AT1G17170 (score: 0.653) AT1G78320 (score: 0.631) AT1G17190 (score: 0.610) AT1G53680 (score: 0.528) AT3G43800 (score: 0.463) AT1G10360 (score: 0.220)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000005211 (score: 1.000; bootstrap: 100%) ENSBTAP00000017435 (score: 0.378)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00039326 (score: 1.000; bootstrap: 52%) WBGene00023684 (score: 0.402)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00001790 (score: 1.000; bootstrap: 53%) WBGene00001791 (score: 0.519) WBGene00021817 (score: 0.437)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000015576 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040718-365 (score: 1.000; bootstrap: 70%) ZDB-CDNA-040718-400 (score: 1.000; bootstrap: 71%) ZDB-CDNA-041114-67 (score: 0.714) ZDB-GENE-041114-67 (score: 0.714)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0035587 (score: 1.000; bootstrap: 46%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA18310-PA (score: 1.000; bootstrap: 51%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000032508 (score: 1.000; bootstrap: 67%)	From Inparanoid:20070104
Homo sapiens	ENSP00000278062 (score: 1.000; bootstrap: 100%) ENSP00000334683 (score: 0.767) ENSP00000345023 (score: 0.610)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000020110 (score: 1.000; bootstrap: 100%) ENSMMUP00000020113 (score: 0.592)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000006704 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1341859 (score: 1.000; bootstrap: 60%)	From Inparanoid:20070104
Oryza gramene	O24595 (score: 1.000; bootstrap: 69%) Q9SES7 (score: 0.672) Q8RW02 (score: 0.664) Q8RW03 (score: 0.662) Q8RW04 (score: 0.659) Q9FQC0 (score: 0.623) Q9ZP61 (score: 0.618) O65032 (score: 0.611) Q9FUE5 (score: 0.547) Q69RL1 (score: 0.539) Q60DP1 (score: 0.407) Q7Y1E0 (score: 0.071)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000005155 (score: 1.000; bootstrap: 100%) ENSPTRP00000026521 (score: 0.765) ENSPTRP00000005157 (score: 0.605)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000017186 (score: 1.000; bootstrap: 100%) ENSRNOP00000016851 (score: 0.499)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000154150 (score: 1.000; bootstrap: 68%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00024925001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000041366 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	SSPA	From SHIGELLACYC
E. coli O157	SSPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00043 Glutathione S-transferase, C-terminal domain
Pfam (EcoliWiki Page)	PF02798 Glutathione S-transferase, N-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:47616
Superfamily (EcoliWiki Page)	SUPERFAMILY:52833
Panther (EcoliWiki Page)	PTHR11260:SF61
EcoCyc	EcoCyc:EG10977	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10977	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000966	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0970	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010594	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

sspA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to sspA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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