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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	smg
Gene Synonym(s)	ECK3271, b3284, JW3245^[1], JW3245
Product Desc.	conserved protein^[2]^[3] Function unknown, after smf gene^[4]
Product Synonyms(s)	conserved protein^[1], B3284^[2]^[1], Smg^[2]^[1] , ECK3271, JW3245, b3284
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): smf-smg^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	smg
Mnemonic	fms backwards, g>f
Synonyms	ECK3271, b3284, JW3245^[1], JW3245
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	73.93 minutes, 73.93 minutes	MG1655: 3430486..3430013 <gbrowseImage> name=NC_000913:3430013..3430486 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3360369..3359896 <gbrowseImage> name=NC_012967:3359896..3360369 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3317170..3317643 <gbrowseImage> name=NC_012759:3317170..3317643 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4207952..4208425 <gbrowseImage> name=NC_007779:4207952..4208425 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3528231..3527758 <gbrowseImage> name=NC_010473:3527758..3528231 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
Δsmg (Keio:JW3245)	deletion	deletion	PMID:16738554	Shigen CGSC10450^[5]
smg::Tn5KAN-I-SceI (FB21096)	Insertion at nt 305 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21096	does not contain pKD46
smg::Tn5KAN-I-SceI (FB21097)	Insertion at nt 393 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21097	contains pKD46
smg-3082::Tn10	Insertion at 3,430,197 bp in MG1655 (NC_000913)		adapted from Nichols et al.^[6]	CAG12071 = CGSC7444^[5]	Synonyms: zhb-3082::Tn10, zhd-3082::Tn10
smg-3082::Tn10			PMID:2540407 PMID:9829956	CGSC:40238
smg-3170::Tn10kan			PMID:2540407	CGSC:40242
Δsmg-723::kan			PMID:16738554	CGSC:102972
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3245	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTCGACGTACTAATGTATTT Primer 2:CCATGCAGCATACCTTCATTCAC
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[5]	est. P1 cotransduction: 58% ^[6] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 1% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11605	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11605	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001553	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947781	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1562	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010770	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Smg
Synonyms	conserved protein^[1], B3284^[2]^[1], Smg^[2]^[1] , ECK3271, JW3245, b3284
Product description	conserved protein^[2]^[3] Function unknown, after smf gene^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	secA	PMID:16606699	Experiment(s):EBI-1145355
Protein	dnaJ	PMID:16606699	Experiment(s):EBI-1145355
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1145355
Protein	rpoC	PMID:16606699	Experiment(s):EBI-1145355
Protein	lon	PMID:16606699	Experiment(s):EBI-1145355
Protein	rplB	PMID:16606699	Experiment(s):EBI-1145355
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1145355
Protein	rpoB	PMID:16606699	Experiment(s):EBI-1145355
Protein	ydbA	PMID:16606699	Experiment(s):EBI-1145355
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFDVLMYLFE TYIHTEAELR VDQDKLEQDL TDAGFEREDI YNALLWLEKL ADYQEGLAEP MQLASDPLSM RIYTPEECER LDASCRGFLL FLEQIQVLNL ETREMVIERV LALDNAEFEL DDLKWVILMV LFNIPGCENA YQQMEELLFE VNEGMLH
Length	157
Mol. Wt	18.51 kDa
pI	3.9 (calculated)
Extinction coefficient	19,940 - 20,315 (calc based on 6 Y, 2 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..157	PF04361 Protein of unknown function (DUF494)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=smg taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131165	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947781	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010770	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A828	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11605	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11605	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947781	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001553	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1562	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	216	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	230	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	252	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
smf-smg
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3430466..3430506 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3284 (EcoliWiki Page)	NCBI GEO profiles for smg
microarray	GenExpDB:b3284 (EcoliWiki Page)	Summary of data for smg from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to smg Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11605	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1562	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3284
EcoGene	EcoGene:EG11605	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001553	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010770	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000029423 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	SMG	From SHIGELLACYC
E. coli O157	SMG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04361 Protein of unknown function (DUF494)
EcoCyc	EcoCyc:EG11605	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11605	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001553	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1562	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010770	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

smg:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to smg Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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