sbcD:On One Page
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;}
h2 .editsection { display:none;}</css>
<protect>
Standard Name |
sbcD |
---|---|
Gene Synonym(s) |
ECK0392, b0398, JW0388, yajA[1], yajA |
Product Desc. |
ATP-dependent dsDNA exonuclease[2][3]; Component of SbcCD ATP-dependent dsDNA exonuclease[3] DNA hairpin dsDNA 3'-exonuclease SbcCD, Mn(2+), ATP-dependent; ATP-independent 5' ssDNA endonuclease; cosuppressor with sbcB of recB recC mutations; heterodimeric[4] |
Product Synonyms(s) |
exonuclease, dsDNA, ATP-dependent[1], B0398[2][1], YajA[2][1], SbcD[2][1] , ECK0392, JW0388, yajA, b0398 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
edit table |
</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
SbcCD can cleave dsDNA 5' ends sealed by a hairpin or by covalent linkage to a protein. SbcCD limits recombination between short inverted repeats, but is also reported to be required for palindrome-stimulated deletion. SbcCD protein forms a large 1.2 megadalton complex.[4]
Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;}
h2 .editsection { display:none;}</css>
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
sbcD |
---|---|
Mnemonic |
Suppression of recBC |
Synonyms |
ECK0392, b0398, JW0388, yajA[1], yajA |
edit table |
</protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
8.94 minutes |
MG1655: 416176..414974 |
||
NC_012967: 385375..384173 |
||||
NC_012759: 317733..318935 |
||||
W3110 |
|
W3110: 416176..414974 |
||
DH10B: 355507..354305 |
||||
edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
414974 |
Edman degradation |
PMID:9242643 |
| |
edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔsbcD (Keio:JW0388) |
deletion |
deletion |
PMID:16738554 |
||||
ΔsbcD762::kan |
PMID:16738554 |
| |||||
edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0388 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCCGCATCCTTCACACCTCAGA Primer 2:CCTGCTTCGTGTTCTCCGGCGAG | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 47% [6] | ||
Linked marker |
est. P1 cotransduction: 56% [6] | ||
edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001082 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB1086 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0001383 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
edit table |
Product(s)
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;}
h2 .editsection { display:none;}</css>
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
SbcD |
---|---|
Synonyms |
exonuclease, dsDNA, ATP-dependent[1], B0398[2][1], YajA[2][1], SbcD[2][1] , ECK0392, JW0388, yajA, b0398 |
Product description |
ATP-dependent dsDNA exonuclease[2][3]; Component of SbcCD ATP-dependent dsDNA exonuclease[3] DNA hairpin dsDNA 3'-exonuclease SbcCD, Mn(2+), ATP-dependent; ATP-independent 5' ssDNA endonuclease; cosuppressor with sbcB of recB recC mutations; heterodimeric[4] |
EC number (for enzymes) |
|
edit table |
<protect></protect>
Notes
Function
<protect>
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0004518 |
nuclease activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0540 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004519 |
endonuclease activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0255 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004527 |
exonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004593 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004527 |
exonuclease activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0269 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006259 |
DNA metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004593 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0235 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0233 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016787 |
hydrolase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004843 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016787 |
hydrolase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0378 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of SbcCD ATP-dependent dsDNA exonuclease |
could be indirect |
||
Protein |
nadE |
PMID:16606699 |
Experiment(s):EBI-1136455 | |
Protein |
phoB |
PMID:16606699 |
Experiment(s):EBI-1136455 | |
Protein |
ydhK |
PMID:16606699 |
Experiment(s):EBI-1136455 | |
edit table |
</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
edit table |
<protect></protect>
Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MRILHTSDWH LGQNFYSKSR EAEHQAFLDW LLETAQTHQV DAIIVAGDVF DTGSPPSYAR TLYNRFVVNL QQTGCHLVVL AGNHDSVATL NESRDIMAFL NTTVVASAGH APQILPRRDG TPGAVLCPIP FLRPRDIITS QAGLNGIEKQ QHLLAAITDY YQQHYADACK LRGDQPLPII ATGHLTTVGA SKSDAVRDIY IGTLDAFPAQ NFPPADYIAL GHIHRAQIIG GMEHVRYCGS PIPLSFDECG KSKYVHLVTF SNGKLESVEN LNVPVTQPMA VLKGDLASIT AQLEQWRDVS QEPPVWLDIE ITTDEYLHDI QRKIQALTES LPVEVLLVRR SREQRERVLA SQQRETLSEL SVEEVFNRRL ALEELDESQQ QRLQHLFTTT LHTLAGEHEA |
Length |
400 |
Mol. Wt |
44.713 kDa |
pI |
5.8 (calculated) |
Extinction coefficient |
38,390 - 39,015 (calc based on 11 Y, 4 W, and 5 C residues) |
edit table |
Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0001383 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120001082 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1086 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
edit table |
Expression
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;}
h2 .editsection { display:none;}</css>
Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MG1655 |
245 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
90 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
88 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
edit table |
Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
</protect>
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:416156..416196
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
</protect>
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for sbcD | |
microarray |
Summary of data for sbcD from multiple microarray studies | |
edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
edit table |
<protect></protect>
Notes
Accessions Related to sbcD Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1086 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001082 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0001383 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
edit table |
Evolution
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;}
h2 .editsection { display:none;}</css>
Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
SBCD |
From SHIGELLACYC |
E. coli O157 |
SBCD |
From ECOO157CYC |
edit table |
Do-It-Yourself Web Tools
<protect></protect>
Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11094 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001082 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1086 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0001383 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
Categories