rpoA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

rpoA

Gene Synonym(s)

ECK3282, b3295, JW3257, phs, sez, pez[1], pez

Product Desc.

RNA polymerase, α subunit[2][3];

Component of RNA polymerase, core enzyme[2][3]; RNA polymerase sigma 24[2][3]; RNA polymerase sigma 38[2][3]; RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]; RNA polymerase sigma54[2]; RNA polymerase sigma 54[3]; RNA polymerase sigma70[2]; RNA polymerase sigma 70[3]; RNA polymerase sigma 19[3]; RNA polymerase sigma 28[3]

RNA polymerase, alpha subunit; binds Zn(II)[4]

Product Synonyms(s)

RNA polymerase, alpha subunit[1], B3295[2][1], Sez[2][1], Phs[2][1], Pez[2][1], RpoA[2][1] , ECK3282, JW3257, pez, phs, sez, b3295

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rpsMKD-rpoA-rplQ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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HT_Cmplx11_Mem: RpoA+RpoB+RpoC. HT_Cmplx2_Cyt: RpoA+RpoB+RpoC.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpoA

Mnemonic

RNA polymerase

Synonyms

ECK3282, b3295, JW3257, phs, sez, pez[1], pez

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.1 minutes, 74.1 minutes 

MG1655: 3439051..3438062
<gbrowseImage> name=NC_000913:3438062..3439051 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3368934..3367945
<gbrowseImage> name=NC_012967:3367945..3368934 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3325219..3326208
<gbrowseImage> name=NC_012759:3325219..3326208 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4199387..4200376
<gbrowseImage> name=NC_007779:4199387..4200376 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3536796..3535807
<gbrowseImage> name=NC_010473:3535807..3536796 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3438062

Edman degradation

PMID:387752
PMID:1095419
PMID:9298646


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rpoAR45C

R45C

In rpoA112; temperature-sensitive, blocks RNA polymerase assembly

seeded from UniProt:P0A7Z4

rpoAR191C

R191C

In rpoA101; temperature-sensitive

seeded from UniProt:P0A7Z4

rpoA112(ts)

temperature sensitive

CGSC:7565


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3257

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCAGGGTTCTGTGACAGAGTT

Primer 2:CCtTCGTCAGCGATGCTTGCCGG

E1H9

Kohara Phage

Genobase

PMID:3038334

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[5]

est. P1 cotransduction: 73% [6]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 3% [6]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10893

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10893

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000884

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947794

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0886

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010802

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RpoA

Synonyms

RNA polymerase, alpha subunit[1], B3295[2][1], Sez[2][1], Phs[2][1], Pez[2][1], RpoA[2][1] , ECK3282, JW3257, pez, phs, sez, b3295

Product description

RNA polymerase, α subunit[2][3];

Component of RNA polymerase, core enzyme[2][3]; RNA polymerase sigma 24[2][3]; RNA polymerase sigma 38[2][3]; RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]; RNA polymerase sigma54[2]; RNA polymerase sigma 54[3]; RNA polymerase sigma70[2]; RNA polymerase sigma 70[3]; RNA polymerase sigma 19[3]; RNA polymerase sigma 28[3]

RNA polymerase, alpha subunit; binds Zn(II)[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00059

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009025

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011260

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011773

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00059

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009025

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011260

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011261

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011262

F

Seeded from EcoCyc (v14.0)

complete

Deprecated

GO:0008270

zinc ion binding

PMID:1985624

IDA: Inferred from Direct Assay

F

The PMID listed is for a paper on spinal cord injury. I cannot find any experimental evidence for Zn binding by the alpha subunit of RNA polymerase. Siegele (talk) 16:16, 15 May 2019 (CDT)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011263

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011773

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.6

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0240

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009025

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011260

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011261

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011262

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011263

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006351

transcription, DNA-dependent

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00059

P

Seeded from EcoCyc (v14.0)

complete

GO:0006351

transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011773

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016779

nucleotidyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0548

F

Seeded from EcoCyc (v14.0)

complete

GO:0046983

protein dimerization activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011261

F

Seeded from EcoCyc (v14.0)

complete

GO:0046983

protein dimerization activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011262

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of RNA polymerase, core enzyme

could be indirect

Protein

aceE

PMID:15690043

Experiment(s):EBI-879354

Protein

aceF

PMID:15690043

Experiment(s):EBI-879354

Protein

aspS

PMID:15690043

Experiment(s):EBI-879354, EBI-883271

Protein

mreB

PMID:15690043

Experiment(s):EBI-879354

Protein

pssA

PMID:15690043

Experiment(s):EBI-879354

Protein

waaU

PMID:15690043

Experiment(s):EBI-879354

Protein

rho

PMID:15690043

Experiment(s):EBI-879354

Protein

rplA

PMID:15690043

Experiment(s):EBI-879354

Protein

rplC

PMID:15690043

Experiment(s):EBI-879354

Protein

rplD

PMID:15690043

Experiment(s):EBI-879354, EBI-883271

Protein

rplE

PMID:15690043

Experiment(s):EBI-879354

Protein

rplM

PMID:15690043

Experiment(s):EBI-879354, EBI-883271

Protein

rplO

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsA

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsB

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsC

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsD

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsE

PMID:15690043

Experiment(s):EBI-879354, EBI-883271

Protein

rpsG

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-879354

Protein

rpsM

PMID:15690043

Experiment(s):EBI-879354

Protein

rplB

PMID:15690043

Experiment(s):EBI-879354

Protein

cspC

PMID:15690043

Experiment(s):EBI-883271

Protein

rplL

PMID:15690043

Experiment(s):EBI-883271

Protein

rplP

PMID:15690043

Experiment(s):EBI-883271

Protein

rplS

PMID:15690043

Experiment(s):EBI-883271

Protein

rplU

PMID:15690043

Experiment(s):EBI-883271

Protein

rplW

PMID:15690043

Experiment(s):EBI-883271

Protein

rplX

PMID:15690043

Experiment(s):EBI-883271

Protein

rpmA

PMID:15690043

Experiment(s):EBI-883271

Protein

rpmB

PMID:15690043

Experiment(s):EBI-883271

Protein

rpmG

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsN

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsO

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsF

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsP

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsS

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsT

PMID:15690043

Experiment(s):EBI-883271

Protein

rpsU

PMID:15690043

Experiment(s):EBI-883271

Protein

wecG

PMID:15690043

Experiment(s):EBI-883271

Protein

rplB

PMID:16606699

Experiment(s):EBI-1145418

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1145418

Protein

hybE

PMID:16606699

Experiment(s):EBI-1145418

Protein

rpsE

PMID:16606699

Experiment(s):EBI-1145418

Protein

pta

PMID:16606699

Experiment(s):EBI-1145418

Protein

etk

PMID:16606699

Experiment(s):EBI-1145418

Protein

rpoB

PMID:16606699

Experiment(s):EBI-1145418

Protein

greA

PMID:16606699

Experiment(s):EBI-1145418

Protein

rplO

PMID:16606699

Experiment(s):EBI-1145418

Protein

rplF

PMID:16606699

Experiment(s):EBI-1145418

Protein

rapA

PMID:16606699

Experiment(s):EBI-1145418

Protein

rpoD

PMID:16606699

Experiment(s):EBI-1145418

Protein

fur

PMID:16606699

Experiment(s):EBI-1145418

Protein

mreB

PMID:19402753

MALDI(Z-score):36.959637

Protein

rpsP

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsS

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplU

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsT

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):3.353874

Protein

rplX

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmG

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753

MALDI(Z-score):21.278856

Protein

rpmB

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplA

PMID:19402753

MALDI(Z-score):32.556213

Protein

rplS

PMID:19402753

LCMS(ID Probability):99.6

Protein

aspS

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):8.467531

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):97.9 MALDI(Z-score):16.475126

Protein

rpsF

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplW

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.053160

Protein

hupA

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):9.189812

Protein

rpsC

PMID:19402753

MALDI(Z-score):41.404786

Protein

rplB

PMID:19402753

MALDI(Z-score):37.610726

Protein

rpsO

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsD

PMID:19402753

MALDI(Z-score):34.513588

Protein

rho

PMID:19402753

MALDI(Z-score):37.049801

Protein

cspC

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):13.185220

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):27.436806

Protein

greA

PMID:19402753

LCMS(ID Probability):93.1 MALDI(Z-score):17.053160

Protein

rpsU

PMID:19402753

LCMS(ID Probability):99.6

Protein

pssA

PMID:19402753

MALDI(Z-score):19.670165

Protein

nusG

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):35.117471

Protein

rpoB

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):39.950272

Protein

rpoC

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):39.702103

Protein

rapA

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):18.240902

Protein

rpoZ

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):38.277175

Protein

rffM

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpoD

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):40.069162

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

Protein

Subunits of RNA polymerase sigma 24

could be indirect

Protein

Subunits of RNA polymerase sigma 38

could be indirect

Protein

Subunits of RNA polymerase sigma32

could be indirect

Protein

Subunits of RNA polymerase sigma54

could be indirect

Protein

Subunits of RNA polymerase sigma70

could be indirect

Protein

Subunits of RNA polymerase sigma 19

could be indirect

Protein

Subunits of RNA polymerase sigma 28

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE
IDGVLHEYST KEGVQEDILE ILLNLKGLAV RVQGKDEVIL TLNKSGIGPV TAADITHDGD
VEIVKPQHVI CHLTDENASI SMRIKVQRGR GYVPASTRIH SEEDERPIGR LLVDACYSPV
ERIAYNVEAA RVEQRTDLDK LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDVRQP
EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL
TEIKDVLASR GLSLGMRLEN WPPASIADE
Length

329

Mol. Wt

36.511 kDa

pI

4.8 (calculated)

Extinction coefficient

12,950 - 13,450 (calc based on 5 Y, 1 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

58..178

PF01000 RNA polymerase Rpb3/RpoA insert domain

PMID:19920124

Domain

243..309

PF03118 Bacterial RNA polymerase, alpha chain C terminal domain

PMID:19920124

Domain

28..233

PF01193 RNA polymerase Rpb3/Rpb11 dimerisation domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rpoA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131174

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947794

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010802

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7Z4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10893

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10893

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947794

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000884

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0886

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

7.87E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 EMG2

850

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: M2-2

PMID: 9298646

Protein

E. coli K-12 MG1655

47625

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

8447

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

24982

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rpsMKD-rpoA-rplQ

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3439031..3439071 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3295 (EcoliWiki Page)

NCBI GEO profiles for rpoA

microarray

GenExpDB:b3295 (EcoliWiki Page)

Summary of data for rpoA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to rpoA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10893

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0886

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3295

EcoGene

EcoGene:EG10893

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000884

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010802

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Oryza gramene

  • Q7GED1 (score: 1.000; bootstrap: 100%)
  • Q6YLW9 (score: 1.000; bootstrap: 100%)
  • Q6YLW3 (score: 1.000; bootstrap: 100%)
  • P12073 (score: 1.000; bootstrap: 100%)
  • Q7GED0 (score: 1.000; bootstrap: 100%)
  • Q6YLW2 (score: 1.000; bootstrap: 100%)
  • P92892 (score: 1.000; bootstrap: 100%)
  • Q7GED9 (score: 1.000; bootstrap: 100%)
  • P92429 (score: 1.000; bootstrap: 100%)
  • Q8MAJ0 (score: 1.000; bootstrap: 100%)
  • Q7H6I6 (score: 1.000; bootstrap: 100%)
  • Q7H6I3 (score: 1.000; bootstrap: 100%)
  • Q7H6I2 (score: 1.000; bootstrap: 100%)
  • Q7H6I1 (score: 1.000; bootstrap: 100%)
  • Q7H6H8 (score: 1.000; bootstrap: 100%)
  • Q7H6H6 (score: 1.000; bootstrap: 100%)
  • Q7H6H1 (score: 1.000; bootstrap: 100%)
  • Q7H6G9 (score: 1.000; bootstrap: 100%)
  • Q7H6G7 (score: 1.000; bootstrap: 100%)
  • Q7H6G6 (score: 1.000; bootstrap: 100%)
  • Q7H6G5 (score: 1.000; bootstrap: 100%)
  • Q7H6G4 (score: 1.000; bootstrap: 100%)
  • Q7H6G2 (score: 1.000; bootstrap: 100%)
  • Q7GED7 (score: 1.000; bootstrap: 100%)
  • Q7GEC5 (score: 1.000; bootstrap: 100%)
  • Q6USB6 (score: 1.000; bootstrap: 100%)
  • P92906 (score: 1.000; bootstrap: 100%)
  • Q7H6G1 (score: 1.000; bootstrap: 100%)
  • Q7H6I5 (score: 1.000; bootstrap: 100%)
  • P93993 (score: 1.000; bootstrap: 100%)
  • P93974 (score: 1.000; bootstrap: 100%)
  • Q7H6I8 (score: 1.000; bootstrap: 100%)
  • Q7H6F7 (score: 1.000; bootstrap: 100%)
  • Q7GEC8 (score: 1.000; bootstrap: 100%)
  • Q539W3 (score: 1.000; bootstrap: 100%)
  • Q539W0 (score: 1.000; bootstrap: 100%)
  • Q539V9 (score: 1.000; bootstrap: 100%)
  • Q539V7 (score: 1.000; bootstrap: 100%)
  • Q539V5 (score: 1.000; bootstrap: 100%)
  • P92903 (score: 1.000; bootstrap: 100%)
  • Q7H6F6 (score: 1.000; bootstrap: 100%)
  • Q6YLW4 (score: 1.000; bootstrap: 100%)
  • Q7GED2 (score: 1.000; bootstrap: 100%)
  • Q7H6H4 (score: 1.000; bootstrap: 100%)
  • Q7H6J3 (score: 1.000; bootstrap: 100%)
  • P93968 (score: 0.998)
  • P92418 (score: 0.998)
  • Q6YLW7 (score: 0.996)
  • Q6YLW6 (score: 0.996)
  • Q8MAI8 (score: 0.996)
  • P92209 (score: 0.996)
  • Q7H6I7 (score: 0.996)
  • Q7GEC7 (score: 0.996)
  • Q7GEC4 (score: 0.996)
  • P92900 (score: 0.996)
  • Q7H6J0 (score: 0.996)
  • Q7H6J1 (score: 0.996)
  • Q7H6J2 (score: 0.996)
  • Q539V4 (score: 0.996)
  • Q6YLV9 (score: 0.995)
  • Q540Z1 (score: 0.995)
  • P92439 (score: 0.995)
  • Q8MAI6 (score: 0.995)
  • P92220 (score: 0.995)
  • P93964 (score: 0.995)
  • Q6YLW5 (score: 0.995)
  • P93956 (score: 0.995)
  • P92392 (score: 0.995)
  • Q8MAI9 (score: 0.991)
  • Q539W2 (score: 0.991)
  • P93960 (score: 0.988)
  • P93962 (score: 0.988)
  • P92389 (score: 0.982)
  • Q539W1 (score: 0.982)
  • P92225 (score: 0.980)
  • Q539V6 (score: 0.922)
  • Q6L370 (score: 0.915)
  • Q6ENT2 (score: 0.915)
  • P09562 (score: 0.915)
  • Q6Z507 (score: 0.891)
  • Q6QY54 (score: 0.891)
  • P12090 (score: 0.891)
  • Q6ENE2 (score: 0.891)
  • Q8S1Q6 (score: 0.888)
  • Q7X6E0 (score: 0.877)
  • Q7DNA8 (score: 0.877)
  • Q8SAU1 (score: 0.875)
  • Q7G6A7 (score: 0.875)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000043398 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

RPOA

From SHIGELLACYC

E. coli O157

RPOA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01000 RNA polymerase Rpb3/RpoA insert domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:47789

Panther (EcoliWiki Page)

PTHR11800:SF4

Superfamily (EcoliWiki Page)

SUPERFAMILY:55257

Superfamily (EcoliWiki Page)

SUPERFAMILY:56553

Pfam (EcoliWiki Page)

PF03118 Bacterial RNA polymerase, alpha chain C terminal domain

Pfam (EcoliWiki Page)

PF01193 RNA polymerase Rpb3/Rpb11 dimerisation domain

EcoCyc

EcoCyc:EG10893

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10893

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000884

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0886

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010802

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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