rpoA:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
RpoA |
|---|---|
| Synonyms |
RNA polymerase, alpha subunit[1], B3295[2][1], Sez[2][1], Phs[2][1], Pez[2][1], RpoA[2][1] , ECK3282, JW3257, pez, phs, sez, b3295 |
| Product description |
RNA polymerase, α subunit[2][3]; Component of RNA polymerase, core enzyme[2][3]; RNA polymerase sigma 24[2][3]; RNA polymerase sigma 38[2][3]; RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]; RNA polymerase sigma54[2]; RNA polymerase sigma 54[3]; RNA polymerase sigma70[2]; RNA polymerase sigma 70[3]; RNA polymerase sigma 19[3]; RNA polymerase sigma 28[3] RNA polymerase, alpha subunit; binds Zn(II)[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003677 |
DNA binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
|
Deprecated |
GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
The PMID listed is for a paper on spinal cord injury. I cannot find any experimental evidence for Zn binding by the alpha subunit of RNA polymerase. Siegele (talk) 16:16, 15 May 2019 (CDT) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006351 |
transcription, DNA-dependent |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006351 |
transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016779 |
nucleotidyltransferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046983 |
protein dimerization activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046983 |
protein dimerization activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of RNA polymerase, core enzyme |
could be indirect |
||
|
Protein |
aceE |
Experiment(s):EBI-879354 | ||
|
Protein |
aceF |
Experiment(s):EBI-879354 | ||
|
Protein |
aspS |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
mreB |
Experiment(s):EBI-879354 | ||
|
Protein |
pssA |
Experiment(s):EBI-879354 | ||
|
Protein |
waaU |
Experiment(s):EBI-879354 | ||
|
Protein |
rho |
Experiment(s):EBI-879354 | ||
|
Protein |
rplA |
Experiment(s):EBI-879354 | ||
|
Protein |
rplC |
Experiment(s):EBI-879354 | ||
|
Protein |
rplD |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
rplE |
Experiment(s):EBI-879354 | ||
|
Protein |
rplM |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
rplO |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsA |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsB |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsC |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsD |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsE |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
rpsG |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsM |
Experiment(s):EBI-879354 | ||
|
Protein |
rplB |
Experiment(s):EBI-879354 | ||
|
Protein |
cspC |
Experiment(s):EBI-883271 | ||
|
Protein |
rplL |
Experiment(s):EBI-883271 | ||
|
Protein |
rplP |
Experiment(s):EBI-883271 | ||
|
Protein |
rplS |
Experiment(s):EBI-883271 | ||
|
Protein |
rplU |
Experiment(s):EBI-883271 | ||
|
Protein |
rplW |
Experiment(s):EBI-883271 | ||
|
Protein |
rplX |
Experiment(s):EBI-883271 | ||
|
Protein |
rpmA |
Experiment(s):EBI-883271 | ||
|
Protein |
rpmB |
Experiment(s):EBI-883271 | ||
|
Protein |
rpmG |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsN |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsO |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsF |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsP |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsS |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsT |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsU |
Experiment(s):EBI-883271 | ||
|
Protein |
wecG |
Experiment(s):EBI-883271 | ||
|
Protein |
rplB |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpsD |
Experiment(s):EBI-1145418 | ||
|
Protein |
hybE |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpsE |
Experiment(s):EBI-1145418 | ||
|
Protein |
pta |
Experiment(s):EBI-1145418 | ||
|
Protein |
etk |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpoB |
Experiment(s):EBI-1145418 | ||
|
Protein |
greA |
Experiment(s):EBI-1145418 | ||
|
Protein |
rplO |
Experiment(s):EBI-1145418 | ||
|
Protein |
rplF |
Experiment(s):EBI-1145418 | ||
|
Protein |
rapA |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpoD |
Experiment(s):EBI-1145418 | ||
|
Protein |
fur |
Experiment(s):EBI-1145418 | ||
|
Protein |
mreB |
MALDI(Z-score):36.959637 | ||
|
Protein |
rpsP |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsS |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplU |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsT |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsN |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplP |
LCMS(ID Probability):99.6 MALDI(Z-score):3.353874 | ||
|
Protein |
rplX |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpmG |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpmA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsA |
MALDI(Z-score):21.278856 | ||
|
Protein |
rpmB |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplA |
MALDI(Z-score):32.556213 | ||
|
Protein |
rplS |
LCMS(ID Probability):99.6 | ||
|
Protein |
aspS |
LCMS(ID Probability):99.6 MALDI(Z-score):8.467531 | ||
|
Protein |
rpsJ |
LCMS(ID Probability):97.9 MALDI(Z-score):16.475126 | ||
|
Protein |
rpsF |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplW |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplM |
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | ||
|
Protein |
hupA |
LCMS(ID Probability):99.6 MALDI(Z-score):9.189812 | ||
|
Protein |
rpsC |
MALDI(Z-score):41.404786 | ||
|
Protein |
rplB |
MALDI(Z-score):37.610726 | ||
|
Protein |
rpsO |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsD |
MALDI(Z-score):34.513588 | ||
|
Protein |
rho |
MALDI(Z-score):37.049801 | ||
|
Protein |
cspC |
LCMS(ID Probability):99.6 MALDI(Z-score):13.185220 | ||
|
Protein |
rplD |
LCMS(ID Probability):99.6 MALDI(Z-score):27.436806 | ||
|
Protein |
greA |
LCMS(ID Probability):93.1 MALDI(Z-score):17.053160 | ||
|
Protein |
rpsU |
LCMS(ID Probability):99.6 | ||
|
Protein |
pssA |
MALDI(Z-score):19.670165 | ||
|
Protein |
nusG |
LCMS(ID Probability):99.6 MALDI(Z-score):35.117471 | ||
|
Protein |
rpoB |
LCMS(ID Probability):99.6 MALDI(Z-score):39.950272 | ||
|
Protein |
rpoC |
LCMS(ID Probability):99.6 MALDI(Z-score):39.702103 | ||
|
Protein |
rapA |
LCMS(ID Probability):99.6 MALDI(Z-score):18.240902 | ||
|
Protein |
rpoZ |
LCMS(ID Probability):99.6 MALDI(Z-score):38.277175 | ||
|
Protein |
rffM |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpoD |
LCMS(ID Probability):99.6 MALDI(Z-score):40.069162 | ||
|
Protein |
rplL |
LCMS(ID Probability):99.6 | ||
|
Protein |
Subunits of RNA polymerase sigma 24 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma 38 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma32 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma54 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma70 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma 19 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma 28 |
could be indirect |
| |
| edit table |
</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE IDGVLHEYST KEGVQEDILE ILLNLKGLAV RVQGKDEVIL TLNKSGIGPV TAADITHDGD VEIVKPQHVI CHLTDENASI SMRIKVQRGR GYVPASTRIH SEEDERPIGR LLVDACYSPV ERIAYNVEAA RVEQRTDLDK LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDVRQP EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL TEIKDVLASR GLSLGMRLEN WPPASIADE |
| Length |
329 |
| Mol. Wt |
36.511 kDa |
| pI |
4.8 (calculated) |
| Extinction coefficient |
12,950 - 13,450 (calc based on 5 Y, 1 W, and 4 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Groomes, TE & Huang, CT (1991) Orthostatic hypotension after spinal cord injury: treatment with fludrocortisone and ergotamine. Arch Phys Med Rehabil 72 56-8 PubMed
- ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 7.23 7.24 7.25 7.26 7.27 7.28 7.29 7.30 7.31 7.32 7.33 7.34 7.35 7.36 7.37 7.38 7.39 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 9.18 9.19 9.20 9.21 9.22 9.23 9.24 9.25 9.26 9.27 9.28 9.29 9.30 9.31 9.32 9.33 9.34 9.35 9.36 9.37 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 10.0 10.1 10.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0003677 ! DNA binding
- GO:0003899 ! DNA-directed 5'-3' RNA polymerase activity
- GO:0008270 ! zinc ion binding
- GO:0006350 ! transcription
- GO:0006351 ! transcription, DNA-templated
- GO:0016020 ! membrane
- GO:0016740 ! transferase activity
- GO:0016779 ! nucleotidyltransferase activity
- GO:0046983 ! protein dimerization activity
- Proteins
