{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	purR
Gene Synonym(s)	ECK1654, b1658, JW1650^[1], JW1650
Product Desc.	PurR^[2]^[3]; Component of PurR transcriptional repressor^[2]^[3]; PurR-Hypoxanthine transcriptional repressor^[2]^[3] Purine regulon repressor^[4]
Product Synonyms(s)	DNA-binding transcriptional repressor, hypoxanthine-binding^[1], B1658^[2]^[1] , ECK1654, JW1650, b1658
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): purR^[2], OP00081
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

The purine regulon includes purBCDEFHKLMN and guaBA.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	purR
Mnemonic	Purine
Synonyms	ECK1654, b1658, JW1650^[1], JW1650
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	37.41 minutes	MG1655: 1735868..1736893 <gbrowseImage> name=NC_000913:1735868..1736893 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1715111..1716136 <gbrowseImage> name=NC_012967:1715111..1716136 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1627927..1628952 <gbrowseImage> name=NC_012759:1627927..1628952 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1739558..1740583 <gbrowseImage> name=NC_007779:1739558..1740583 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔpurR (Keio:JW1650)	deletion	deletion			PMID:16738554	Shigen CGSC9404^[5]
purR::Tn5KAN-2 (FB20410)	Insertion at nt 804 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20410	does not contain pKD46
purR::Tn5KAN-2 (FB20411)	Insertion at nt 804 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20411	contains pKD46
purRL54M		L54M		Slight decrease in operator DNA affinity			seeded from UniProt:P0ACP7
purRK55A		K55A		Decrease in operator DNA affinity			seeded from UniProt:P0ACP7
purRW147A		W147A		14-fold increase in corepressor affinity. Large increase in repressor activity			seeded from UniProt:P0ACP7
purRW147F		W147F		Large decrease in corepressor affinity and in repressor activity			seeded from UniProt:P0ACP7
purRW147R		W147R		8-fold increase in corepressor affinity. Large increase in repressor activity			seeded from UniProt:P0ACP7
purRR190A		R190A		Functional repressor. Corepressor specificity is expanded since it allows binding of adenine and 6-methylpurine			seeded from UniProt:P0ACP7
purRR190Q		R190Q		Functional repressor. Corepressor specificity is expanded since it allows binding of adenine			seeded from UniProt:P0ACP7
ΔpurR::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
purR96						CGSC:25131
purR97						CGSC:26539
purR106::Tn10						CGSC:86254
ΔpurR746::kan					PMID:16738554	CGSC:102544
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1650	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAACAATAAAAGATGTAGC Primer 2:CCACGACGATAGTCGCGGAACGG
6F11	Kohara Phage	Genobase	PMID:3038334
4H6	Kohara Phage	Genobase	PMID:3038334
13H4	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:zdh-925::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10800	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10800	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000791	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945226	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0793	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005545	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PurR
Synonyms	DNA-binding transcriptional repressor, hypoxanthine-binding^[1], B1658^[2]^[1] , ECK1654, JW1650, b1658
Product description	PurR^[2]^[3]; Component of PurR transcriptional repressor^[2]^[3]; PurR-Hypoxanthine transcriptional repressor^[2]^[3] Purine regulon repressor^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010982	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006164	purine nucleotide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016564	transcription repressor activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01277	F	Seeded from EcoCyc (v14.0)	complete
	GO:0045892	negative regulation of transcription, DNA-dependent	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01277	P	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of PurR transcriptional repressor	could be indirect
Protein	Subunits of PurR-Hypoxanthine transcriptional repressor	could be indirect
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MATIKDVAKR ANVSTTTVSH VINKTRFVAE ETRNAVWAAI KELHYSPSAV ARSLKVNHTK SIGLLATSSE AAYFAEIIEA VEKNCFQKGY TLILGNAWNN LEKQRAYLSM MAQKRVDGLL VMCSEYPEPL LAMLEEYRHI PMVVMDWGEA KADFTDAVID NAFEGGYMAG RYLIERGHRE IGVIPGPLER NTGAGRLAGF MKAMEEAMIK VPESWIVQGD FEPESGYRAM QQILSQPHRP TAVFCGGDIM AMGALCAADE MGLRVPQDVS LIGYDNVRNA RYFTPALTTI HQPKDSLGET AFNMLLDRIV NKREEPQSIE VHPRLIERRS VADGPFRDYR R
Length	341
Mol. Wt	38.174 kDa
pI	6.7 (calculated)
Extinction coefficient	39,880 - 40,380 (calc based on 12 Y, 4 W, and 4 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0ACP7
Domain	3..48	PF00356 Bacterial regulatory proteins, lacI family		PMID:19920124
Domain	59..329	PF00532 family		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purR taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129616	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945226	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005545	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACP7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10800	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10800	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945226	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000791	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0793	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	4.05E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	4198	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1217	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2026	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
purR
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:1735848..1735888 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1658 (EcoliWiki Page)	NCBI GEO profiles for purR
microarray	GenExpDB:b1658 (EcoliWiki Page)	Summary of data for purR from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to purR Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10800	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0793	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1658
EcoGene	EcoGene:EG10800	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000791	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005545	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000034998 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000047522 (score: 1.000; bootstrap: 89%)	From Inparanoid:20070104
Shigella flexneri	PURR	From SHIGELLACYC
E. coli O157	PURR	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00356 Bacterial regulatory proteins, lacI family
Superfamily (EcoliWiki Page)	SUPERFAMILY:47413
Superfamily (EcoliWiki Page)	SUPERFAMILY:53822
Pfam (EcoliWiki Page)	PF00532 family
EcoCyc	EcoCyc:EG10800	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10800	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000791	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0793	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005545	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

purR:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to purR Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search