purR:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
purR |
|---|---|
| Mnemonic |
Purine |
| Synonyms |
ECK1654, b1658, JW1650[1], JW1650 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
37.41 minutes |
MG1655: 1735868..1736893 |
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NC_012967: 1715111..1716136 |
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NC_012759: 1627927..1628952 |
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W3110 |
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W3110: 1739558..1740583 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔpurR (Keio:JW1650) |
deletion |
deletion |
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|
purR::Tn5KAN-2 (FB20410) |
Insertion at nt 804 in Plus orientation |
does not contain pKD46 | |||||
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purR::Tn5KAN-2 (FB20411) |
Insertion at nt 804 in Plus orientation |
contains pKD46 | |||||
|
purRL54M |
L54M |
Slight decrease in operator DNA affinity |
seeded from UniProt:P0ACP7 | ||||
|
purRK55A |
K55A |
Decrease in operator DNA affinity |
seeded from UniProt:P0ACP7 | ||||
|
purRW147A |
W147A |
14-fold increase in corepressor affinity. Large increase in repressor activity |
seeded from UniProt:P0ACP7 | ||||
|
purRW147F |
W147F |
Large decrease in corepressor affinity and in repressor activity |
seeded from UniProt:P0ACP7 | ||||
|
purRW147R |
W147R |
8-fold increase in corepressor affinity. Large increase in repressor activity |
seeded from UniProt:P0ACP7 | ||||
|
purRR190A |
R190A |
Functional repressor. Corepressor specificity is expanded since it allows binding of adenine and 6-methylpurine |
seeded from UniProt:P0ACP7 | ||||
|
purRR190Q |
R190Q |
Functional repressor. Corepressor specificity is expanded since it allows binding of adenine |
seeded from UniProt:P0ACP7 | ||||
|
ΔpurR::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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purR96 |
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purR97 |
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|
purR106::Tn10 |
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ΔpurR746::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1650 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCAACAATAAAAGATGTAGC Primer 2:CCACGACGATAGTCGCGGAACGG | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: % [8] | ||
|
zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: 17% [8] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
