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Standard Name	purN
Gene Synonym(s)	ECK2496, b2500, JW2485, adec, ade(c)^[1], ade
Product Desc.	GART^[2]^[3] Glycinamide ribonucleotide transformylase (GART) 1, purine synthesis; glycinamide ribonucleotide formyltransferase^[4]
Product Synonyms(s)	phosphoribosylglycinamide formyltransferase 1^[1], B2500^[2]^[1], PurN^[2]^[1] , ade, ECK2496, JW2485, b2500
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): purMN^[2], OP00080, purM
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	purN
Mnemonic	Purine
Synonyms	ECK2496, b2500, JW2485, adec, ade(c)^[1], ade
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	56.47 minutes	MG1655: 2620256..2620894 <gbrowseImage> name=NC_000913:2620256..2620894 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2543054..2543692 <gbrowseImage> name=NC_012967:2543054..2543692 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2506061..2506699 <gbrowseImage> name=NC_012759:2506061..2506699 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2620890..2621528 <gbrowseImage> name=NC_007779:2620890..2621528 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2712021..2712659 <gbrowseImage> name=NC_010473:2712021..2712659 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2620256	Edman degradation	PMID:2204419
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔpurN (Keio:JW2485)	deletion	deletion		PMID:16738554	Shigen CGSC9984^[5]
purNH108A,G,M,N,Q,R		H108A,G,M,N,Q,R	Loss of activity			seeded from UniProt:P08179
purNH108E,S,T		H108E,S,T	Reduces activity about 1000- fold			seeded from UniProt:P08179
purNH119A		H119A	No effect			seeded from UniProt:P08179
purNH121Q		H121Q	Increases Km for 5'- phosphoribosylglycinamide 4-fold			seeded from UniProt:P08179
purNS135A,L		S135A,L	Reduces activity about 1000-fold			seeded from UniProt:P08179
purNH137F,Q		H137F,Q	No effect			seeded from UniProt:P08179
purNE70A		E70A	Loss of homodimerization. No effect on activity			seeded from UniProt:P08179
purNN106A,D,G,S		N106A,D,G,S	Reduces activity about 2000- fold			seeded from UniProt:P08179
purNN106E,H,I,K,L,Y		N106E,H,I,K,L,Y	Loss of activity			seeded from UniProt:P08179
purND144A,E,S,Y		D144A,E,S,Y	Reduces activity about 1000- fold			seeded from UniProt:P08179
purND144C,F,H,K,L,N,P,Q,R,T,V		D144C,F,H,K,L,N,P,Q,R,T,V	Loss of activity			seeded from UniProt:P08179
ΔpurN748::kan				PMID:16738554	CGSC:102542
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2485	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATATTGTGGTGCTTATTTC Primer 2:CCtTCGTCGGCAGCGTAGCCCTG
10H6	Kohara Phage	Genobase	PMID:3038334
5A11	Kohara Phage	Genobase	PMID:3038334
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[5]	est. P1 cotransduction: 36% ^[6]
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 16% ^[6] Synonyms:zff-208::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10799	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10799	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000790	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946973	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0792	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008232	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PurN
Synonyms	phosphoribosylglycinamide formyltransferase 1^[1], B2500^[2]^[1], PurN^[2]^[1] , ade, ECK2496, JW2485, b2500
Product description	GART^[2]^[3] Glycinamide ribonucleotide transformylase (GART) 1, purine synthesis; glycinamide ribonucleotide formyltransferase^[4]
EC number (for enzymes)	2.1.2.2^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004644	phosphoribosylglycinamide formyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004607	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004644	phosphoribosylglycinamide formyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.1.2.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006164	purine nucleotide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006189	'de novo' IMP biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004607	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008168	methyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001555	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002376	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016742	hydroxymethyl-, formyl- and related transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002376	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNIVVLISGN GSNLQAIIDA CKTNKIKGTV RAVFSNKADA FGLERARQAG IATHTLIASA FDSREAYDRE LIHEIDMYAP DVVVLAGFMR ILSPAFVSHY AGRLLNIHPS LLPKYPGLHT HRQALENGDE EHGTSVHFVT DELDGGPVIL QAKVPVFAGD SEDDITARVQ TQEHAIYPLV ISWFADGRLK MHENAAWLDG QRLPPQGYAA DE
Length	212
Mol. Wt	23.238 kDa
pI	5.8 (calculated)
Extinction coefficient	19,940 - 20,065 (calc based on 6 Y, 2 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..181	PF00551 Formyl transferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purN taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130425	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946973	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008232	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P08179	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10799	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10799	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946973	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000790	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0792	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.98E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	753	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	426	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	214	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
purMN
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2620236..2620276 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2500 (EcoliWiki Page)	NCBI GEO profiles for purN
microarray	GenExpDB:b2500 (EcoliWiki Page)	Summary of data for purN from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to purN Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10799	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0792	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2500
EcoGene	EcoGene:EG10799	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000790	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008232	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001057 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G31220 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0230085 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6ZK11 (score: 1.000; bootstrap: 100%) Q53WN6 (score: 0.655) Q69XB6 (score: 0.392)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDR408C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC5698c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000035533 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Shigella flexneri	PURN	From SHIGELLACYC
E. coli O157	PURN	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00551 Formyl transferase
Superfamily (EcoliWiki Page)	SUPERFAMILY:53328
Panther (EcoliWiki Page)	PTHR10520:SF9
EcoCyc	EcoCyc:EG10799	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10799	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000790	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0792	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008232	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

purN:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to purN Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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