purN:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
purN |
|---|---|
| Mnemonic |
Purine |
| Synonyms |
ECK2496, b2500, JW2485, adec, ade(c)[1], ade |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
56.47 minutes |
MG1655: 2620256..2620894 |
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NC_012967: 2543054..2543692 |
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NC_012759: 2506061..2506699 |
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W3110 |
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W3110: 2620890..2621528 |
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DH10B: 2712021..2712659 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2620256 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔpurN (Keio:JW2485) |
deletion |
deletion |
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purNH108A,G,M,N,Q,R |
H108A,G,M,N,Q,R |
Loss of activity |
seeded from UniProt:P08179 | ||||
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purNH108E,S,T |
H108E,S,T |
Reduces activity about 1000- fold |
seeded from UniProt:P08179 | ||||
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purNH119A |
H119A |
No effect |
seeded from UniProt:P08179 | ||||
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purNH121Q |
H121Q |
Increases Km for 5'- phosphoribosylglycinamide 4-fold |
seeded from UniProt:P08179 | ||||
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purNS135A,L |
S135A,L |
Reduces activity about 1000-fold |
seeded from UniProt:P08179 | ||||
|
purNH137F,Q |
H137F,Q |
No effect |
seeded from UniProt:P08179 | ||||
|
purNE70A |
E70A |
Loss of homodimerization. No effect on activity |
seeded from UniProt:P08179 | ||||
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purNN106A,D,G,S |
N106A,D,G,S |
Reduces activity about 2000- fold |
seeded from UniProt:P08179 | ||||
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purNN106E,H,I,K,L,Y |
N106E,H,I,K,L,Y |
Loss of activity |
seeded from UniProt:P08179 | ||||
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purND144A,E,S,Y |
D144A,E,S,Y |
Reduces activity about 1000- fold |
seeded from UniProt:P08179 | ||||
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purND144C,F,H,K,L,N,P,Q,R,T,V |
D144C,F,H,K,L,N,P,Q,R,T,V |
Loss of activity |
seeded from UniProt:P08179 | ||||
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ΔpurN748::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2485 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAATATTGTGGTGCTTATTTC Primer 2:CCtTCGTCGGCAGCGTAGCCCTG | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 36% [7] | ||
|
Linked marker |
est. P1 cotransduction: 16% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Inglese, J et al. (1990) Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli. Biochemistry 29 6678-87 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
