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Gene |

Product(s) |

Expression|

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Standard Name	purK
Gene Synonym(s)	ECK0515, b0522, JW0511, purE2^[1], purE2
Product Desc.	N5-carboxyaminoimidazole ribonucleotide synthase^[2]^[3]; Component of N5-carboxyaminoimidazole ribonucleotide synthase^[2]^[3] N5-carboxyaminoimidazole ribonucleotide (N-CAIR) synthase; purine synthesis^[4]
Product Synonyms(s)	N5-carboxyaminoimidazole ribonucleotide synthase^[1], B0522^[2]^[1], PurK^[2]^[1], purE2 peptide^[2]^[1], CO2-binding subunit^[2]^[1], AIR carboxylase^[2]^[1] , ECK0515, JW0511, purE2, b0522
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): purEK^[2], OP00112, purE
Regulation/Activity
Quick Links
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Notes

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PurK was previously thought to be the ATPase subunit of phosphoribosylaminoimidazole carboxylase, with catalytic subunit PurE (Tiedeman, 1989). PurK is a separate N-CAIR synthase (Mueller, 1994).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	purK
Mnemonic	Purine
Synonyms	ECK0515, b0522, JW0511, purE2^[1], purE2
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	11.87 minutes	MG1655: 551817..550750 <gbrowseImage> name=NC_000913:550750..551817 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 524702..523635 <gbrowseImage> name=NC_012967:523635..524702 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 453510..454577 <gbrowseImage> name=NC_012759:453510..454577 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 551817..550750 <gbrowseImage> name=NC_007779:550750..551817 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 491149..490082 <gbrowseImage> name=NC_010473:490082..491149 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	550750	Edman degradation	PMID:1534690
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
purK(del) (Keio:JW0511)	deletion	deletion	PMID:16738554	Shigen CGSC8655^[5]
purK::Tn5KAN-2 (FB20194)	Insertion at nt 472 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20194	does not contain pKD46
purK79::Tn10	Insertion at 551,279 bp in MG1655 (NC_000913)		adapted from Nichols et al.^[6]	CAG12171 = CGSC7342^[5]	Synonyms: purE79::Tn10
purK79::Tn10			PMID:9829956 PMID:2540407 PMID:3540312	CGSC:7112
purK0				CGSC:25087
purK3200::Tn10kan			PMID:2540407 PMID:9829956	CGSC:39381
purK735(del)::kan			PMID:16738554	CGSC:102536
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0511	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACAGGTTTGCGTCCTCGG Primer 2:CCACCGAACTTACTCTGCGCCCA
6E7	Kohara Phage	Genobase	PMID:3038334
gsk-3055::Tn10	Linked marker	CAG12154 = CGSC7341^[5]	est. P1 cotransduction: 10% ^[6] Synonyms:zbb-30-55::TnlO, zba-3055::Tn10
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10796	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10796	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000787	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945153	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0789	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001794	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PurK
Synonyms	N5-carboxyaminoimidazole ribonucleotide synthase^[1], B0522^[2]^[1], PurK^[2]^[1], purE2 peptide^[2]^[1], CO2-binding subunit^[2]^[1], AIR carboxylase^[2]^[1] , ECK0515, JW0511, purE2, b0522
Product description	N5-carboxyaminoimidazole ribonucleotide synthase^[2]^[3]; Component of N5-carboxyaminoimidazole ribonucleotide synthase^[2]^[3] N5-carboxyaminoimidazole ribonucleotide (N-CAIR) synthase; purine synthesis^[4]
EC number (for enzymes)	4.1.1.21^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011761	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013816	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013817	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016185	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004638	phosphoribosylaminoimidazole carboxylase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005875	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004638	phosphoribosylaminoimidazole carboxylase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.1.21	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011761	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013816	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013817	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016185	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006164	purine nucleotide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006189	'de novo' IMP biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005875	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016831	carboxy-lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0210	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004638	phosphoribosylaminoimidazole carboxylase activity	PMID:8117684	IDA: Inferred from Direct Assay		F	Figure 6. NMR analysis demonstrates that PurK converts AIR to N5-CAIR as part of a direct assay.	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of N5-carboxyaminoimidazole ribonucleotide synthase	could be indirect
Protein	dinG		PMID:16606699	Experiment(s):EBI-1137033
Protein	phnN		PMID:16606699	Experiment(s):EBI-1137033
Protein	nadE		PMID:16606699	Experiment(s):EBI-1137033
Protein	tatE		PMID:16606699	Experiment(s):EBI-1137033
Protein	gfcD		PMID:16606699	Experiment(s):EBI-1137033
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKQVCVLGNG QLGRMLRQAG EPLGIAVWPV GLDAEPAAVP FQQSVITAEI ERWPETALTR ELARHPAFVN RDVFPIIADR LTQKQLFDKL HLPTAPWQLL AERSEWPAVF DRLGELAIVK RRTGGYDGRG QWRLRANETE QLPAECYGEC IVEQGINFSG EVSLVGARGF DGSTVFYPLT HNLHQDGILR TSVAFPQANA QQQAQAEEML SAIMQELGYV GVMAMECFVT PQGLLINELA PRVHNSGHWT QNGASISQFE LHLRAITDLP LPQPVVNNPS VMINLIGSDV NYDWLKLPLV HLHWYDKEVR PGRKVGHLNL TDSDTSRLTA TLEALIPLLP PEYASGVIWA QSKFG
Length	355
Mol. Wt	39.46 kDa
pI	5.8 (calculated)
Extinction coefficient	59,930 - 60,430 (calc based on 7 Y, 9 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	86..254	PF02222 ATP-grasp domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purK taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128506	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945153	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001794	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P09029	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10796	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10796	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945153	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000787	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0789	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	6.13E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	655	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	745	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	194	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
purEK
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:551797..551837 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0522 (EcoliWiki Page)	NCBI GEO profiles for purK
microarray	GenExpDB:b0522 (EcoliWiki Page)	Summary of data for purK from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to purK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10796	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0789	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0522
EcoGene	EcoGene:EG10796	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000787	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001794	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	PURK	From SHIGELLACYC
E. coli O157	PURK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02222 ATP-grasp domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:51246
Superfamily (EcoliWiki Page)	SUPERFAMILY:52440
Panther (EcoliWiki Page)	PTHR23047:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:56059
EcoCyc	EcoCyc:EG10796	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10796	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000787	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0789	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001794	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

purK:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to purK Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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