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Gene |

Product(s) |

Expression|

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Standard Name	purE
Gene Synonym(s)	ECK0516, b0523, JW0512, Pur2, ade3, adef, ade(f)^[1], ade
Product Desc.	PurE^[2]^[3]; Component of N5-carboxyaminoimidazole ribonucleotide mutase^[2]^[3] N5-carboxyaminoimidazole ribonucleotide (N-CAIR) mutase; purine synthesis^[4]
Product Synonyms(s)	N5-carboxyaminoimidazole ribonucleotide mutase^[1], B0523^[2]^[1], PurE^[2]^[1], purE1 peptide^[2]^[1], catalytic subunit^[2]^[1], N5-CAIR mutase^[2]^[1], AIR carboxylase subunit^[2]^[1], N5-carboxyaminoimidazole ribonucleotide mutase subunit^[2]^[1] , ade, ade3, ECK0516, JW0512, Pur2, b0523
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): purEK^[2], OP00112, purE
Regulation/Activity
Quick Links
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Notes

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PurE was previously thought to be the catalytic subunit of phosphoribosylaminoimidazole carboxylase, with ATPase subunit PurK (Tiedeman, 1989). PurE is a separate N-CAIR mutase (Mueller, 1994; Meyer, 1999).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	purE
Mnemonic	Purine
Synonyms	ECK0516, b0523, JW0512, Pur2, ade3, adef, ade(f)^[1], ade
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	11.89 minutes	MG1655: 552323..551814 <gbrowseImage> name=NC_000913:551814..552323 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 525208..524699 <gbrowseImage> name=NC_012967:524699..525208 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 454574..455083 <gbrowseImage> name=NC_012759:454574..455083 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 552323..551814 <gbrowseImage> name=NC_007779:551814..552323 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 491655..491146 <gbrowseImage> name=NC_010473:491146..491655 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	551817	Edman degradation	PMID:1534690
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
purE(del) (Keio:JW0512)	deletion	deletion	PMID:16738554	Shigen CGSC8656^[5]
purE::Tn5KAN-2 (FB20195)	Insertion at nt 251 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20195	does not contain pKD46
purE42				CGSC:5375
purE55				CGSC:6363
purE41				CGSC:8185
purE43				CGSC:10256
purE0				CGSC:12048
purE83				CGSC:14356
purE736(del)::kan			PMID:16738554	CGSC:102532
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0512	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTTCCCGCAATAATCCGGC Primer 2:CCTGCCGCACCTCGCGGGTCCGG
6E7	Kohara Phage	Genobase	PMID:3038334
gsk-3055::Tn10	Linked marker	CAG12154 = CGSC7341^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zbb-30-55::TnlO, zba-3055::Tn10
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 99% ^[6] Synonyms:purE79::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10793	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10793	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000784	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949031	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0786	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001796	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PurE
Synonyms	N5-carboxyaminoimidazole ribonucleotide mutase^[1], B0523^[2]^[1], PurE^[2]^[1], purE1 peptide^[2]^[1], catalytic subunit^[2]^[1], N5-CAIR mutase^[2]^[1], AIR carboxylase subunit^[2]^[1], N5-carboxyaminoimidazole ribonucleotide mutase subunit^[2]^[1] , ade, ade3, ECK0516, JW0512, Pur2, b0523
Product description	PurE^[2]^[3]; Component of N5-carboxyaminoimidazole ribonucleotide mutase^[2]^[3] N5-carboxyaminoimidazole ribonucleotide (N-CAIR) mutase; purine synthesis^[4]
EC number (for enzymes)	4.1.1.21^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004638	phosphoribosylaminoimidazole carboxylase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000031	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004638	phosphoribosylaminoimidazole carboxylase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.1.21	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0006164	purine nucleotide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0658	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006189	'de novo' IMP biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000031	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016831	carboxy-lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0210	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of N5-carboxyaminoimidazole ribonucleotide mutase	could be indirect
Protein	Q79BD2		PMID:16606699	Experiment(s):EBI-1137040
Protein	nadE		PMID:16606699	Experiment(s):EBI-1137040
Protein	tatE		PMID:16606699	Experiment(s):EBI-1137040
Protein	ispG		PMID:16606699	Experiment(s):EBI-1137040
Protein	otsB		PMID:16606699	Experiment(s):EBI-1137040
Protein	gabT		PMID:16606699	Experiment(s):EBI-1137040
Protein	zwf		PMID:16606699	Experiment(s):EBI-1137040
Protein	napC		PMID:16606699	Experiment(s):EBI-1137040
Protein	degP		PMID:16606699	Experiment(s):EBI-1137040
Protein	yibL		PMID:16606699	Experiment(s):EBI-1137040
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSSRNNPARV AIVMGSKSDW ATMQFAAEIF EILNVPHHVE VVSAHRTPDK LFSFAESAEE NGYQVIIAGA GGAAHLPGMI AAKTLVPVLG VPVQSAALSG VDSLYSIVQM PRGIPVGTLA IGKAGAANAA LLAAQILATH DKELHQRLND WRKAQTDEVL ENPDPRGAA
Length	169
Mol. Wt	17.779 kDa
pI	6.5 (calculated)
Extinction coefficient	13,980 (calc based on 2 Y, 2 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0AG18
Domain	8..159	PF00731 AIR carboxylase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128507	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949031	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001796	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AG18	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10793	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10793	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949031	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000784	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0786	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.66E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1068	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1194	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	210	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
purEK
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:552303..552343 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0523 (EcoliWiki Page)	NCBI GEO profiles for purE
microarray	GenExpDB:b0523 (EcoliWiki Page)	Summary of data for purE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to purE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10793	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0786	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0523
EcoGene	EcoGene:EG10793	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000784	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001796	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G37690 (score: 1.000; bootstrap: 100%) AT2G05140 (score: 0.132)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0230088 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q5QN02 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YOR128C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC13223 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PURE	From SHIGELLACYC
E. coli O157	PURE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00731 AIR carboxylase
Superfamily (EcoliWiki Page)	SUPERFAMILY:52255
Panther (EcoliWiki Page)	PTHR23046:SF0
EcoCyc	EcoCyc:EG10793	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10793	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000784	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0786	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001796	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

purE:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to purE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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