ptsH:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ptsH

Mnemonic

Phosphotransferase system

Synonyms

ECK2410, b2415, JW2408, hpr, ctr[1], ctr, Hpr, iex?

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

54.57 minutes 

MG1655: 2531786..2532043
<gbrowseImage> name=NC_000913:2531786..2532043 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2462490..2462747
<gbrowseImage> name=NC_012967:2462490..2462747 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2417591..2417848
<gbrowseImage> name=NC_012759:2417591..2417848 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2539210..2539467
<gbrowseImage> name=NC_007779:2539210..2539467 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2623551..2623808
<gbrowseImage> name=NC_010473:2623551..2623808 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2531786

Edman degradation

PMID:9298646[2]
PMID:9868784[3]
PMID:10094700[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ptsH(del) (Keio:JW2408)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC9917[6]

ptsH::Tn5KAN-2 (FB20788)

Insertion at nt 37 in Minus orientation

PMID:15262929[7]

E. coli Genome Project:FB20788

contains pKD46

ptsH1

CGSC:7995

ptsH744(del)::kan

PMID:16738554[5]

CGSC:102520

ptsH in strain E187

Resistant to

Resistant to phosphonomycin

PMID:4555418[8]

Strain:E187

Table 1

ptsHI in strain L191

Resistant to

Double mutant is resistant to streptozotocin

PMID:161156[9]

Strain: L191

Table 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2408

Plasmid clone

Shigen

PMID:16769691[10]

Status:Clone OK

Primer 1:GCCTTCCAGCAAGAAGTTACCAT

Primer 2:CCtTCGAGTTCCGCCATCAGTTT

12G2

Kohara Phage

Genobase

PMID:3038334[11]

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[6]

est. P1 cotransduction: 45% [12]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[6]

est. P1 cotransduction: 4% [12]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10788

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10788

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000779

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946886

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0781

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007962

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. Wasinger, VC & Humphery-Smith, I (1998) Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. 169 375-82 PubMed
  4. Blankenhorn, D et al. (1999) Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J. Bacteriol. 181 2209-16 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  8. Venkateswaran, PS & Wu, HC (1972) Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J. Bacteriol. 110 935-44 PubMed
  9. Ammer, J et al. (1979) Phosphorylation of streptozotocin during uptake via the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli. Antimicrob. Agents Chemother. 16 801-7 PubMed
  10. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  11. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  12. 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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