PMID:4555418
| Citation |
Venkateswaran, PS and Wu, HC (1972) Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J. Bacteriol. 110:935-44 |
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| Abstract |
A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of (3)H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation. |
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| Keywords |
Anti-Bacterial Agents/pharmacology; Carbon Isotopes; Cell Wall/metabolism; Chromatography, Paper; Culture Media; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Genetics, Microbial; Glucose/metabolism; Glucosephosphates/metabolism; Glycerophosphates/metabolism; Microscopy, Phase-Contrast; Mutation; Phosphoenolpyruvate; Phosphotransferases/metabolism; Pimelic Acids/metabolism; Temperature; Tritium |
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