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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	pssA
Gene Synonym(s)	ECK2583, b2585, JW2569, pss^[1], pss
Product Desc.	PssA^[2]^[3]; Component of phosphatidylserine synthase^[2]^[3] Phosphatidylserine synthase^[4]
Product Synonyms(s)	phosphatidylserine synthase (CDP-diacylglycerol-serine O-phosphatidyltransferase)^[1], B2585^[2]^[1], Pss^[2]^[1], PssA^[2]^[1] , ECK2583, JW2569, pss, b2585
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pssA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pssA
Mnemonic	Phosphatidylserine synthase
Synonyms	ECK2583, b2585, JW2569, pss^[1], pss
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	58.64 minutes	MG1655: 2720749..2722104 <gbrowseImage> name=NC_000913:2720749..2722104 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2644220..2645575 <gbrowseImage> name=NC_012967:2644220..2645575 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2606554..2607909 <gbrowseImage> name=NC_012759:2606554..2607909 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2721383..2722738 <gbrowseImage> name=NC_007779:2721383..2722738 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2812514..2813869 <gbrowseImage> name=NC_010473:2812514..2813869 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2720749	Edman degradation	PMID:2002065
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
pssA1				CGSC:8523
pssA21(ts)		temperature sensitive		CGSC:7540
pssA allege in strain UB1005	Resistant to	ethanol resistance.	PMID:92527	Experimental strain: DC11	Data is not shown.
pssA allele in strain UB1005	Resistant to	Resistance to Dimenthyl sulfoxide.	PMID:92527	Experimental Strain: DC11	Data is not shown.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2569	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTGTCAAAATTTAAGCGTAA Primer 2:CCaAGGATGCGGCTAATTAATCG
3F10	Kohara Phage	Genobase	PMID:3038334
21D7	Kohara Phage	Genobase	PMID:3038334
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 68% ^[6]
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: 55% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10781	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10781	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000772	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947059	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0774	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008510	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PssA
Synonyms	phosphatidylserine synthase (CDP-diacylglycerol-serine O-phosphatidyltransferase)^[1], B2585^[2]^[1], Pss^[2]^[1], PssA^[2]^[1] , ECK2583, JW2569, pss, b2585
Product description	PssA^[2]^[3]; Component of phosphatidylserine synthase^[2]^[3] Phosphatidylserine synthase^[4]
EC number (for enzymes)	2.7.8.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of phosphatidylserine synthase	could be indirect
Protein	pnp		PMID:15690043	Experiment(s):EBI-887767
Protein	rplC		PMID:15690043	Experiment(s):EBI-887767
Protein	rpsA		PMID:15690043	Experiment(s):EBI-887767
Protein	rpsB		PMID:15690043	Experiment(s):EBI-887767
Protein	rpsC		PMID:15690043	Experiment(s):EBI-887767
Protein	rpsD		PMID:15690043	Experiment(s):EBI-887767
Protein	ycbY		PMID:15690043	Experiment(s):EBI-887767
Protein	yfiF		PMID:15690043	Experiment(s):EBI-887767, EBI-879491
Protein	rplL		PMID:15690043	Experiment(s):EBI-893445
Protein	rplU		PMID:15690043	Experiment(s):EBI-893445
Protein	rplW		PMID:15690043	Experiment(s):EBI-893445
Protein	rpsE		PMID:15690043	Experiment(s):EBI-893445
Protein	rpsG		PMID:15690043	Experiment(s):EBI-893445
Protein	rpsN		PMID:15690043	Experiment(s):EBI-893445
Protein	rpsU		PMID:15690043	Experiment(s):EBI-893445
Protein	ppiD		PMID:15690043	Experiment(s):EBI-893445
Protein	rpsD		PMID:16606699	Experiment(s):EBI-1143395
Protein	rplB		PMID:16606699	Experiment(s):EBI-1143395
Protein	yghW		PMID:16606699	Experiment(s):EBI-1143395
Protein	ppx		PMID:16606699	Experiment(s):EBI-1143395
Protein	rpsM		PMID:16606699	Experiment(s):EBI-1143395
Protein	rplA		PMID:16606699	Experiment(s):EBI-1143395
Protein	rplQ		PMID:16606699	Experiment(s):EBI-1143395
Protein	ppiD		PMID:19402753	LCMS(ID Probability):99.6
Protein	rplW		PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsA		PMID:19402753	MALDI(Z-score):24.335358
Protein	rpsN		PMID:19402753	LCMS(ID Probability):99.6
Protein	rplU		PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsU		PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):3.277672
Protein	rlmL		PMID:19402753	MALDI(Z-score):26.896684
Protein	rpsD		PMID:19402753	MALDI(Z-score):33.362536
Protein	rpsC		PMID:19402753	MALDI(Z-score):28.422938
Protein	yfiF		PMID:19402753	MALDI(Z-score):34.756829
Protein	rplL		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMLSKFKRNK HQQHLAQLPK ISQSVDDVDF FYAPADFRET LLEKIASAKQ RICIVALYLE QDDGGKGILN ALYEAKRQRP ELDVRVLVDW HRAQRGRIGA AASNTNADWY CRMAQENPGV DVPVYGVPIN TREALGVLHF KGFIIDDSVL YSGASLNDVY LHQHDKYRYD RYHLIRNRKM SDIMFEWVTQ NIMNGRGVNR LDDVNRPKSP EIKNDIRLFR QELRDAAYHF QGDADNDQLS VTPLVGLGKS SLLNKTIFHL MPCAEQKLTI CTPYFNLPAI LVRNIIQLLR EGKKVEIIVG DKTANDFYIP EDEPFKIIGA LPYLYEINLR RFLSRLQYYV NTDQLVVRLW KDDDNTYHLK GMWVDDKWML ITGNNLNPRA WRLDLENAIL IHDPQLELAP QREKELELIR EHTTIVKHYR DLQSIADYPV KVRKLIRRLR RIRIDRLISR IL
Length	452
Mol. Wt	52.934 kDa
pI	9.3 (calculated)
Extinction coefficient	68,300 - 68,800 (calc based on 20 Y, 7 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	352..379	PF00614 Phospholipase D Active site motif		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pssA taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111464	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947059	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008510	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P23830	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10781	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10781	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947059	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000772	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0774	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.76E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	94.049+/-0.458	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.38656+/-0.02925	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.117343173	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1855	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	535	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	845	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pssA
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2720729..2720769 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2585 (EcoliWiki Page)	NCBI GEO profiles for pssA
microarray	GenExpDB:b2585 (EcoliWiki Page)	Summary of data for pssA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to pssA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10781	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0774	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2585
EcoGene	EcoGene:EG10781	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000772	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008510	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000021095 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000000897 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00021677 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000007937 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000005778 (score: 1.000; bootstrap: 100%) ENSCINP00000004333 (score: 0.331)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0217874 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0038649 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA20541-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000011616 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000004007 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YCL004W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBP18G52 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000021982 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PSSA	From SHIGELLACYC
E. coli O157	PSSA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00614 Phospholipase D Active site motif
Panther (EcoliWiki Page)	PTHR12586:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:56024
Superfamily (EcoliWiki Page)	SUPERFAMILY:56024
EcoCyc	EcoCyc:EG10781	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10781	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000772	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0774	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008510	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

pssA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pssA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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