pssA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pssA

Mnemonic

Phosphatidylserine synthase

Synonyms

ECK2583, b2585, JW2569, pss[1], pss

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

58.64 minutes 

MG1655: 2720749..2722104
<gbrowseImage> name=NC_000913:2720749..2722104 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2644220..2645575
<gbrowseImage> name=NC_012967:2644220..2645575 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2606554..2607909
<gbrowseImage> name=NC_012759:2606554..2607909 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2721383..2722738
<gbrowseImage> name=NC_007779:2721383..2722738 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2812514..2813869
<gbrowseImage> name=NC_010473:2812514..2813869 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2720749

Edman degradation

PMID:2002065[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

pssA1

CGSC:8523

pssA21(ts)

temperature sensitive

CGSC:7540

pssA allege in strain UB1005

Resistant to

ethanol resistance.

PMID:92527[3]

Experimental strain: DC11

Data is not shown.

pssA allele in strain UB1005

Resistant to

Resistance to Dimenthyl sulfoxide.

PMID:92527[3]

Experimental Strain: DC11

Data is not shown.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2569

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTTGTCAAAATTTAAGCGTAA

Primer 2:CCaAGGATGCGGCTAATTAATCG

3F10

Kohara Phage

Genobase

PMID:3038334[5]

21D7

Kohara Phage

Genobase

PMID:3038334[5]

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[6]

est. P1 cotransduction: 68% [7]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[6]

est. P1 cotransduction: 55% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10781

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10781

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000772

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947059

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0774

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008510

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. DeChavigny, A et al. (1991) Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability. J. Biol. Chem. 266 5323-32 PubMed
  3. 3.0 3.1 Clark, DP & Beard, JP (1979) Altered phospholipid composition in mutants of Escherichia coli sensitive or resistant to organic solvents. J. Gen. Microbiol. 113 267-74 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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