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Gene |

Product(s) |

Expression|

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Standard Name	pspA
Gene Synonym(s)	ECK1299, b1304, JW1297, cog^[1], cog
Product Desc.	regulatory protein for the phage shock protein operon^[2]^[3] Phage shock protein A, negative regulator of psp operon; stress-inducible; binds PspB and PspC; enhances protein export through Tat pathway^[4]
Product Synonyms(s)	regulatory protein for phage-shock-protein operon^[1], B1304^[2]^[1], Cog^[2]^[1], PspA^[2]^[1], phage shock protein^[2]^[1] , cog, ECK1299, JW1297, b1304
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pspABCDE^[2], pspABCE
Regulation/Activity
Quick Links
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Notes

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A pspA deletion grows slow below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60). Co-expression of pspA with pspC mitigates the growth inhibition by pspC; pspA-pspC has been suggested to be a possible toxin-antitoxin pair (Brown, 2003).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pspA
Mnemonic	Phage shock protein
Synonyms	ECK1299, b1304, JW1297, cog^[1], cog
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	29.44 minutes	MG1655: 1366103..1366771 <gbrowseImage> name=NC_000913:1366103..1366771 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1366447..1367115 <gbrowseImage> name=NC_012967:1366447..1367115 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1256952..1257620 <gbrowseImage> name=NC_012759:1256952..1257620 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1369793..1370461 <gbrowseImage> name=NC_007779:1369793..1370461 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1455499..1456167 <gbrowseImage> name=NC_010473:1455499..1456167 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1366106	Edman degradation	PMID:8501036 PMID:9493373
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
pspA(del) (Keio:JW1297)	deletion	deletion			PMID:16738554	Shigen CGSC9162^[5]
pspA740(del)::kan					PMID:16738554	CGSC:102492
pspABCDE in strain 1B1			Resistant to	Sulfanilamide + hypoxanthine resistance	PMID:6392473
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1297	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGGTATTTTTTCTCGCTTTGC Primer 2:CCTTGATTGTCTTGCTTCATTTT
1C2	Kohara Phage	Genobase	PMID:3038334
2D3	Kohara Phage	Genobase	PMID:3038334
trpC83::Tn10	Linked marker	CAG18455 = CGSC7371^[5]	est. P1 cotransduction: 11% ^[6] Synonyms:trpB83::Tn10
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 92% ^[6] Synonyms:zci-233::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10776	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10776	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000767	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945887	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0769	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004387	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PspA
Synonyms	regulatory protein for phage-shock-protein operon^[1], B1304^[2]^[1], Cog^[2]^[1], PspA^[2]^[1], phage shock protein^[2]^[1] , cog, ECK1299, JW1297, b1304
Product description	regulatory protein for the phage shock protein operon^[2]^[3] Phage shock protein A, negative regulator of psp operon; stress-inducible; binds PspB and PspC; enhances protein export through Tat pathway^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006950	response to stress	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009408	response to heat	PMID:8349564	IEP: Inferred from Expression Pattern		P		complete
	GO:0009271	phage shock	PMID:2105503	IEP: Inferred from Expression Pattern		P	Induced by Phage f1 protein IV	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	crl		PMID:16606699	Experiment(s):EBI-1139527
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Notes

Purified PspA forms oligomers that interact with phosphatidylserine and phosphatidylglycerol lipid headgroups, and stabilize membranes against proton leakage^[7]

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MGIFSRFADI VNANINALLE KAEDPQKLVR LMIQEMEDTL VEVRSTSARA LAEKKQLTRR IEQASAREVE WQEKAELALL KEREDLARAA LIEKQKLTDL IKSLEHEVTL VDDTLARMKK EIGELENKLS ETRARQQALM LRHQAANSSR DVRRQLDSGK LDEAMARFES FERRIDQMEA EAESHSFGKQ KSLDDQFAEL KADDAISEQL AQLKAKMKQD NQ
Length	222
Mol. Wt	25.493 kDa
pI	5.3 (calculated)
Extinction coefficient	5,500 (calc based on 0 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0AFM6
Domain	2..219	PF04012 PspA/IM30 family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pspA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129265	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945887	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004387	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFM6	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10776	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10776	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945887	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000767	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0769	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	8.92E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1652	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	602	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1143	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pspABCDE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1366083..1366123 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1304 (EcoliWiki Page)	NCBI GEO profiles for pspA
microarray	GenExpDB:b1304 (EcoliWiki Page)	Summary of data for pspA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to pspA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10776	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0769	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1304
EcoGene	EcoGene:EG10776	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000767	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004387	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G65260 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000023111 (score: 1.000; bootstrap: 100%) ENSBTAP00000000932 (score: 0.305) ENSBTAP00000013737 (score: 0.075) ENSBTAP00000030962 (score: 0.073) ENSBTAP00000008510 (score: 0.071) ENSBTAP00000009781 (score: 0.061) ENSBTAP00000004905 (score: 0.058) ENSBTAP00000004733 (score: 0.051)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00016611 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10507-PA (score: 1.000; bootstrap: 100%) GA12841-PA (score: 0.358)	From Inparanoid:20070104
Oryza gramene	Q8S0J7 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000152497 (score: 1.000; bootstrap: 51%) NEWSINFRUP00000127930 (score: 1.000; bootstrap: 53%) NEWSINFRUP00000161948 (score: 0.113) NEWSINFRUP00000136665 (score: 0.096)	From Inparanoid:20070104
Shigella flexneri	PSPA	From SHIGELLACYC
E. coli O157	PSPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04012 PspA/IM30 family
EcoCyc	EcoCyc:EG10776	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10776	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000767	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0769	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004387	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Kobayashi, R et al. (2007) Escherichia coli phage-shock protein A (PspA) binds to membrane phospholipids and repairs proton leakage of the damaged membranes. Mol. Microbiol. 66 100-9 PubMed

pspA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pspA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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