pspA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pspA

Mnemonic

Phage shock protein

Synonyms

ECK1299, b1304, JW1297, cog[1], cog

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

29.44 minutes 

MG1655: 1366103..1366771
<gbrowseImage> name=NC_000913:1366103..1366771 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1366447..1367115
<gbrowseImage> name=NC_012967:1366447..1367115 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1256952..1257620
<gbrowseImage> name=NC_012759:1256952..1257620 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1369793..1370461
<gbrowseImage> name=NC_007779:1369793..1370461 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1455499..1456167
<gbrowseImage> name=NC_010473:1455499..1456167 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1366106

Edman degradation

PMID:8501036[2]
PMID:9493373[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

pspA(del) (Keio:JW1297)

deletion

deletion

PMID:16738554[4]

Shigen

CGSC9162[5]

pspA740(del)::kan

PMID:16738554[4]

CGSC:102492

pspABCDE in strain 1B1

Resistant to

Sulfanilamide + hypoxanthine resistance

PMID:6392473[6]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1297

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGGTATTTTTTCTCGCTTTGC

Primer 2:CCTTGATTGTCTTGCTTCATTTT

1C2

Kohara Phage

Genobase

PMID:3038334[8]

2D3

Kohara Phage

Genobase

PMID:3038334[8]

trpC83::Tn10

Linked marker

CAG18455 = CGSC7371[5]

est. P1 cotransduction: 11% [9]
Synonyms:trpB83::Tn10

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[5]

est. P1 cotransduction: 92% [9]
Synonyms:zci-233::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10776

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10776

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000767

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945887

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0769

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004387

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Carlson, JH & Silhavy, TJ (1993) Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. J. Bacteriol. 175 3327-34 PubMed
  3. Kobayashi, H et al. (1998) Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents. Microbiology (Reading, Engl.) 144 ( Pt 2) 353-9 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Bruce, I et al. (1984) Potentiation by purines of the growth-inhibitory effects of sulphonamides on Escherichia coli K12 and the location of the gene which mediates this effect. J. Gen. Microbiol. 130 2489-95 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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