ppsA:Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ppsA |
|---|---|
| Mnemonic |
PEP synthase |
| Synonyms |
ECK1700, b1702, JW1692, ppsAA, pps[1], pps |
| edit table |
</protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
38.42 minutes |
MG1655: 1785136..1782758 |
||
|
NC_012967: 1762002..1764380 |
||||
|
NC_012759: 1674817..1677195 |
||||
|
W3110 |
|
W3110: 1788826..1786448 |
||
|
DH10B: 1875707..1873329 |
||||
| edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1782761 |
Edman degradation |
| ||
| edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Δpps (Keio:JW1692) |
deletion |
deletion |
|||||
|
ppsA::Tn5KAN-2 (FB20430) |
Insertion at nt 1043 in Plus orientation |
contains pKD46 | |||||
|
ppsA::Tn5KAN-2 (FB20430) |
Insertion at nt 1043 in Plus orientation |
does not contain pKD46 | |||||
|
ΔppsA::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
||||
|
ΔppsA::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
||||
|
ΔppsA::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
||||
|
ΔppsA::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
||||
|
ΔppsA::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
||||
|
ΔppsA::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
||||
|
pps-1 |
|||||||
|
pps-3 |
|||||||
|
pps-4 |
|||||||
|
pps-6 |
|||||||
|
pps-2 |
|||||||
|
Δpps-776::kan |
| ||||||
| edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1692 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCCAACAATGGCTCGTCACC Primer 2:CCTTTCTTCAGTTCAGCCAGGCT | |
|
Kohara Phage |
|||
|
zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: 83% [9] | |
|
Linked marker |
est. P1 cotransduction: 10% [9] | ||
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
<protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Niersbach, M et al. (1992) Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase. Mol. Gen. Genet. 231 332-6 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
