PMID:1310524
| Citation |
Niersbach, M, Kreuzaler, F, Geerse, RH, Postma, PW and Hirsch, HJ (1992) Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase. Mol. Gen. Genet. 231:332-6 |
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| Abstract |
We have cloned and sequenced the Escherichia coli K-12 ppsA gene. The ppsA gene codes for PEP synthase, which converts pyruvate into phosphoenolpyruvate (PEP), an essential step in gluconeogenesis when pyruvate or lactate are used as a carbon source. The open reading frame consists of 792 amino acids and shows homology with other phosphohistidine-containing enzymes that catalyze the conversion between pyruvate and PEP. These enzymes include pyruvate, orthophosphate dikinases from plants and Bacteroides symbiosus and Enzyme I of the bacterial PEP:carbohydrate phosphotransferase system. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Histidine/analogs & derivatives; Histidine/genetics; Molecular Sequence Data; Pyruvate, Orthophosphate Dikinase/chemistry; Pyruvate, Orthophosphate Dikinase/genetics; Sequence Alignment; Sequence Homology, Nucleic Acid |
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