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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	plsC
Gene Synonym(s)	ECK3009, b3018, JW2986, parF^[1], parF
Product Desc.	1-ACYLGLYCEROL-3-P- 1-Acyl-n-glycerol-3-phosphate acyltransferase; affects partitioning^[2]
Product Synonyms(s)	1-acyl-sn-glycerol-3-phosphate acyltransferase^[1], B3018^[3]^[1], ParF^[3]^[1], PlsC^[3]^[1] , ECK3009, JW2986, parF, b3018
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): plsC^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	plsC
Mnemonic	Phospholipid synthesis
Synonyms	ECK3009, b3018, JW2986, parF^[1], parF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	68.12 minutes	MG1655: 3161503..3160766 <gbrowseImage> name=NC_000913:3160766..3161503 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3097653..3096916 <gbrowseImage> name=NC_012967:3096916..3097653 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3047914..3048651 <gbrowseImage> name=NC_012759:3047914..3048651 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3162137..3161400 <gbrowseImage> name=NC_007779:3161400..3162137 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3259248..3258511 <gbrowseImage> name=NC_010473:3258511..3259248 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3160766	Edman degradation	PMID:1557036
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
plsC101					PMID:2211622	CGSC:63654
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Notes

plsC is essential, based on failure to isolate plasmid insertions that disrupt the gene^[4]

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2986	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTATATATCTTTCGTCTTAT Primer 2:CCAACTTTTCCGGCGGCTTCGCG
6H4	Kohara Phage	Genobase	PMID:3038334
17B2	Kohara Phage	Genobase	PMID:3038334
metC162::Tn10	Linked marker	CAG18475 = CGSC7435^[5]	est. P1 cotransduction: 70% ^[6]
tolC210::Tn10	Linked marker	CAG12184 = CGSC7437^[5]	est. P1 cotransduction: 54% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11377	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11377	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001345	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947496	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1351	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009910	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PlsC
Synonyms	1-acyl-sn-glycerol-3-phosphate acyltransferase^[1], B3018^[3]^[1], ParF^[3]^[1], PlsC^[3]^[1] , ECK3009, JW2986, parF, b3018
Product description	1-ACYLGLYCEROL-3-P- 1-Acyl-n-glycerol-3-phosphate acyltransferase; affects partitioning^[2]
EC number (for enzymes)	2.3.1.51^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0031225	anchored to membrane				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003841	1-acylglycerol-3-phosphate O-acyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004552	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003841	1-acylglycerol-3-phosphate O-acyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.3.1.51	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002123	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008415	acyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002123	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008415	acyltransferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0012	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008654	phospholipid biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004552	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008654	phospholipid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0594	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004552	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016747	transferase activity, transferring acyl groups other than amino-acyl groups	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.3.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	rplL		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Inner Membrane	PMID:1557036	EchoLocation:plsC
plasma membrane		From EcoCyc^[7]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLYIFRLIIT VIYSILVCVF GSIYCLFSPR NPKHVATFGH MFGRLAPLFG LKVECRKPTD AESYGNAIYI ANHQNNYDMV TASNIVQPPT VTVGKKSLLW IPFFGQLYWL TGNLLIDRNN RTKAHGTIAE VVNHFKKRRI SIWMFPEGTR SRGRGLLPFK TGAFHAAIAA GVPIIPVCVS TTSNKINLNR LHNGLVIVEM LPPIDVSQYG KDQVRELAAH CRSIMEQKIA ELDKEVAERE AAGKV
Length	245
Mol. Wt	27.453 kDa
pI	10.0 (calculated)
Extinction coefficient	28,420 - 29,045 (calc based on 8 Y, 3 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	52..180	PF01553 Acyltransferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=plsC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130914	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947496	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009910	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P26647	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11377	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11377	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947496	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001345	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1351	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	862	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	174	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	368	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
plsC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3161483..3161523 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3018 (EcoliWiki Page)	NCBI GEO profiles for plsC
microarray	GenExpDB:b3018 (EcoliWiki Page)	Summary of data for plsC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3161411..3161736) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ09; Well:A9^[8]
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Notes

Accessions Related to plsC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11377	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1351	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3018
EcoGene	EcoGene:EG11377	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001345	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009910	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000011242 (score: 1.000; bootstrap: 98%) ENSANGP00000018852 (score: 0.170) ENSANGP00000018875 (score: 0.127)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000024518 (score: 1.000; bootstrap: 100%) ENSAPMP00000023580 (score: 0.403)	From Inparanoid:20070104
Arabidopsis thaliana	AT4G30580 (score: 1.000; bootstrap: 52%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000005821 (score: 1.000; bootstrap: 100%) ENSBTAP00000035108 (score: 0.177)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00027436 (score: 1.000; bootstrap: 100%) WBGene00036088 (score: 0.100)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00011543 (score: 1.000; bootstrap: 100%) WBGene00010339 (score: 0.110)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000001104 (score: 1.000; bootstrap: 100%) ENSCAFP00000028942 (score: 0.153)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0183825 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0026718 (score: 1.000; bootstrap: 100%) FBgn0030421 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA14576-PA (score: 1.000; bootstrap: 100%) GA17703-PA (score: 0.102)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000000375 (score: 1.000; bootstrap: 100%) ENSGALP00000001452 (score: 0.354)	From Inparanoid:20070104
Homo sapiens	ENSP00000337463 (score: 1.000; bootstrap: 100%) ENSP00000312239 (score: 0.294)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000024254 (score: 1.000; bootstrap: 100%) ENSMMUP00000027618 (score: 0.279)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000018622 (score: 1.000; bootstrap: 100%) ENSMODP00000021952 (score: 0.256)	From Inparanoid:20070104
Mus musculus	MGI:1932075 (score: 1.000; bootstrap: 100%) MGI:1914762 (score: 0.265)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000030753 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000000500 (score: 1.000; bootstrap: 100%) ENSRNOP00000026408 (score: 0.218)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDL052C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC18512 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000139818 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000011398 (score: 1.000; bootstrap: 96%) ENSXETP00000001688 (score: 0.147) ENSXETP00000025384 (score: 0.111)	From Inparanoid:20070104
Shigella flexneri	PLSC	From SHIGELLACYC
E. coli O157	PLSC	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01553 Acyltransferase
Panther (EcoliWiki Page)	PTHR10434:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:69593
EcoCyc	EcoCyc:EG11377	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11377	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001345	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1351	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009910	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Kato, J et al. (1988) Gene organization in the region containing a new gene involved in chromosome partition in Escherichia coli. J. Bacteriol. 170 3967-77 PubMed
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

plsC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to plsC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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