{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	plaP
Gene Synonym(s)	ECK2008, b2014, JW5330^[1], yeeF, JW5330
Product Desc.	YeeF amino acid APC transporter^[2]^[3] Required for swarming phenotype, function unknown; predicted transporter^[4]
Product Synonyms(s)	predicted amino-acid transporter^[1], B2014^[2]^[1], YeeF^[2]^[1] , PlaP, ECK2008, JW5330, b2014
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yeeF^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	plaP
Mnemonic	Systematic nomenclature
Synonyms	ECK2008, b2014, JW5330^[1], yeeF, JW5330
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	44.91 minutes	MG1655: 2085086..2083728 <gbrowseImage> name=NC_000913:2083728..2085086 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2017505..2016147 <gbrowseImage> name=NC_012967:2016147..2017505 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1976211..1977569 <gbrowseImage> name=NC_012759:1976211..1977569 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2089199..2087841 <gbrowseImage> name=NC_007779:2087841..2089199 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2176094..2174736 <gbrowseImage> name=NC_010473:2174736..2176094 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
yeeF(del) (Keio:JW5330)	deletion	deletion		PMID:16738554	Shigen CGSC11323^[5]
yeeF::Tn5KAN-I-SceI (FB20572)	Insertion at nt 544 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20572	contains pKD46
yeeF783(del)::kan				PMID:16738554	CGSC:104498
yeef(del)	deletion	deletion	Single knock-out mutant shows impaired putrescine uptake, fig 5.	PMID:21266585
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5330	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCGCATAACGTTACTCCAAA Primer 2:CCTGCTACGTCTTCGTACTGCGG
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[5]	est. P1 cotransduction: 98% ^[6] Synonyms:zee-3129::Tn10
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11896	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11896	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001826	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946533	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1842	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006696	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PlaP
Synonyms	predicted amino-acid transporter^[1], B2014^[2]^[1], YeeF^[2]^[1] , PlaP, ECK2008, JW5330, b2014
Product description	YeeF amino acid APC transporter^[2]^[3] Required for swarming phenotype, function unknown; predicted transporter^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004841	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002293	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0029	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015171	amino acid transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002293	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002293	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004841	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002293	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004841	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015489	putrescine transmembrane transporter activity	PMID:21266585	IGI: Inferred from Genetic Interaction	UniProtKB:P0AFK4 UniProtKB:P0AFK6 UniProtKB:P0AFK9 UniProtKB:P0AFR9 UniProtKB:P21169 UniProtKB:P21170 UniProtKB:P60651 UniProtKB:P69874 UniProtKB:P76108 UniProtKB:P77156 UniProtKB:P77795	F	see Fig 4.	complete
	GO:0048870	cell motility	PMID:21266585	IGI: Inferred from Genetic Interaction	UniProtKB:P0AFK4 UniProtKB:P0AFK6 UniProtKB:P0AFK9 UniProtKB:P0AFR9 UniProtKB:P21169 UniProtKB:P21170 UniProtKB:P60651 UniProtKB:P69874 UniProtKB:P76108 UniProtKB:P77156 UniProtKB:P77795	P		complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTMSHNVTPN TSRVELRKTL TLVPVVMMGL AYMQPMTLFD TFGIVSGLTD GHVPTAYAFA LIAILFTALS YGKLVRRYPS AGSAYTYAQK SISPTVGFMV GWSSLLDYLF APMINILLAK IYFEALVPSI PSWMFVVALV AFMTAFNLRS LKSVANFNTV IVVLQVVLIA VILGMVVYGV FEGEGAGTLA STRPFWSGDA HVIPMITGAT ILCFSFTGFD GISNLSEETK DAERVIPRAI FLTALIGGMI FIFATYFLQL YFPDISRFKD PDASQPEIML YVAGKAFQVG ALIFSTITVL ASGMAAHAGV ARLMYVMGRD GVFPKSFFGY VHPKWRTPAM NIILVGAIAL LAINFDLVMA TALINFGALV AFTFVNLSVI SQFWIREKRN KTLKDHFQYL FLPMCGALTV GALWVNLEES SMVLGLIWAA IGLIYLACVT KSFRNPVPQY EDVA
Length	454
Mol. Wt	49.771 kDa
pI	9.0 (calculated)
Extinction coefficient	63,830 - 64,205 (calc based on 17 Y, 7 W, and 3 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	22..438	PF00324 Amino acid permease		PMID:19920124
edit table

<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yeeF taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111370	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946533	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006696	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AA47	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11896	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11896	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946533	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001826	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1842	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.92E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	18110	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1081	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	4030	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yeeF
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:2085066..2085106 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2014 (EcoliWiki Page)	NCBI GEO profiles for yeeF
microarray	GenExpDB:b2014 (EcoliWiki Page)	Summary of data for yeeF from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to yeeF Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11896	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1842	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2014
EcoGene	EcoGene:EG11896	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001826	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006696	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000016290 (score: 1.000; bootstrap: 100%) ENSANGP00000016984 (score: 0.339)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000012050 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00016806 (score: 1.000; bootstrap: 100%) WBGene00017747 (score: 0.456) WBGene00015197 (score: 0.455)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000018906 (score: 1.000; bootstrap: 100%) ENSCINP00000016866 (score: 0.384) ENSCINP00000002795 (score: 0.277)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-030919-2 (score: 1.000; bootstrap: 86%) ZDB-CDNA-041114-206 (score: 0.303) ZDB-GENE-041114-206 (score: 0.303) ZDB-CDNA-041212-7 (score: 0.300) ZDB-GENE-041212-5 (score: 0.300)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0185190 (score: 1.000; bootstrap: 89%) DDB0189300 (score: 1.000; bootstrap: 82%) DDB0205912 (score: 0.262)	From Inparanoid:20070104
Mus musculus	MGI:2146512 (score: 1.000; bootstrap: 100%) MGI:88117 (score: 0.314) MGI:99828 (score: 0.295) MGI:1100521 (score: 0.294) MGI:3040688 (score: 0.229)	From Inparanoid:20070104
Oryza gramene	Q7X636 (score: 1.000; bootstrap: 87%) Q53QH5 (score: 0.448) Q9LD96 (score: 0.282) Q5Z6S8 (score: 0.266) Q6AST5 (score: 0.208)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC5843 (score: 1.000; bootstrap: 64%) SPCC744 (score: 0.493) SPAC10391 (score: 0.441) SPBC15C44c (score: 0.210) SPAC11D38c (score: 0.167) SPAPB24D32c (score: 0.148) SPAC90 (score: 0.068) SPCC7943 (score: 0.052)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000140033 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000132890 (score: 0.503) NEWSINFRUP00000151033 (score: 0.502) NEWSINFRUP00000136276 (score: 0.311) NEWSINFRUP00000135371 (score: 0.302)	From Inparanoid:20070104
Shigella flexneri	YEEF	From SHIGELLACYC
E. coli O157	YEEF	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00324 Amino acid permease
EcoCyc	EcoCyc:EG11896	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11896	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001826	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1842	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006696	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

plaP:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yeeF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search